Silver Nanoparticles Synthesised Using Sargassum Angustifolium Can Effect on MiR-25 and MiR-143 Expression in Acute Lymphoblastic Leukemia
-
Sepideh Valipour
,
Narges Obeidi
,
Gholamreza Khamisipour
,
Seyed Amin Mohammadi
,
Maryam Ghaemi
,
Farzad Farhangdoost
,
Hamideh Malekhayaty
,
Maedeh Darzipour
Abstract
Background: One of the treatments of acute lymphoblastic leukemia (ALL) is chemotherapy but it can destroy other normal cells as well as cancer cells, which can lead to infection and bleeding. The widespread consumption of nanoparticles synthesized biogenically by seaweed is because of their easy accessibility and usefulness. We aimed to investigate silver nanoparticles synthesised using Sargassum on the expression of miR-25 and 143 in Jurkat cell line.
Methods: In an interventional study in 2019, Bushehr, Iran, following culture of the Jurkat cell line with 95% survival rate, we applied different drug concentrations to 20,000 cell lines and control cells separately to determine maximum cell growth inhibition and IC50. After treatment for 48 h, RNA extraction was performed, and Real Time PCR was used to evaluate miR-25 and 143 expressions.
Results: MiR-25 expression in the groups that treated with the maximum dose and IC50 of silver nanoparticles and algae extract was decline but did not differ significantly from that of the control group. Conversely, miR-143 showed a remarkable decline in both treatment groups (P<0.0001). After treating PBMC with nanoparticles the expression level of miR-25 and miR-143were not significantly different.
Conclusion: The level of miR-25 and 143 expressions were decrease in the IC50 dosage of silver nanoparticles and algae extract produced. The level of miR-25 expression decreased more in the treated Jurkat cell line than the level of miR-143 expression in normal lymphocytes. Given the oncogenicity of miR-25 in the Jurkat cell line, it is suggested that decreasing level of this microRNA can help to apoptosis of leukemic cells.
1. Terwilliger T, Abdul-Hay M (2017). Acute lymphoblastic leukemia: a comprehensive review and 2017 update. Blood Cancer J, 7(6):e577-e577.
2. Vijayakrishnan J, Studd J, Broderick P, et al (2018). Genome-wide association study identifies susceptibility loci for B-cell childhood acute lymphoblastic leukemia. Nat Commun,9(1):1340-1348.
3. Bhojwani D, Yang JJ, Pui CH (2015). Biology of childhood acute lymphoblastic leukemia. Pediatr Clin North Am,62(1):47-60.
4. Belver L, Ferrando A (2016). The genetics and mechanisms of T cell acute lymphoblastic leukaemia. Nat Rev Cancer, 16(8):494-507.
5. Coniglio SJ, Blackman JA (1995). Developmental outcome of childhood leukemia. Topics Early Child Spec Educ,15(1):19-31.
6. Clapé C, Fritz V, Henriquet C, et al (2009). miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice. PLoS One, 4(10):e7542.
7. Vannini I, Fanini F, Fabbri M (2018). Emerging roles of microRNAs in can-cer. Curr Opin Genet Dev,48:128-133.
8. Shokri G, Kouhkan F, Nojehdehi S, et al (2019). Simultaneous regulation of miR-451 and miR-191 led to erythroid fate decision of mouse embryonic stem cell. Iran J Basic Med Sci,22(4):432-438.
9. Papagiannopoulos CI, Theodoroula NF, Vizirianakis IS (2021). miR-16-5p promotes erythroid maturation of erythroleukemia cells by regulating ribosome biogenesis. Pharmaceuticals (Basel),14(2):137.
10. Liu L, Yu X, Guo X, et al (2012). miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2. Mol Med Rep, 5(3):753-60.
11. Parsa N (2012). Cellular and molecular basis of cancer in humans. Cell & Tissue Journal ,2(4):365-76.
12. Xu Y, Yang J, Li X (2016). MicroRNA-25 promotes T-cell acute lymphoblastic leukemia cell proliferation and invasion by directly targeting EphA8. Int J Clin Exp Pathol,9(5):5292-8.
13. Akao Y, Nakagawa Y, Iio A, Naoe T (2009). Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells. Leuk Res, 33(11):1530-8.
14. Pugazhendhi A, Prabhu R, Muruganantham K, et al (2019). Anticancer, antimicrobial and photocatalytic activities of green synthesized magnesium oxide nanoparticles (MgONPs) using aqueous extract of Sargassum wightii. J Photochem Photobiol B,190:86-97.
15. Kumar P, Senthamil Selvi S, Lakshmi Prabha A, et al (2012). Synthesis of silver nanoparticles from Sargassum tenerrimum and screening phytochemicals for its antibacterial activity. Nano Biomed Eng,4(1):12-16.
16. Ghaemi M, Gholamipoor S (2017). Controllable synthesis and characterization of silver nanoparticles using Sargassum angostifolium. Iran J Chem Chem Eng, 36(1):1-10
17. Gu Y, Liang Y, Gong J, et al (2012). Suitable internal control microRNA genes for measuring miRNA abundance in pig milk during different lactation periods. Genet and Mol Res,11(3):2506-12.
18. Palanisamy S, Rajasekar P, Vijayaprasath G, et al (2017). A green route to synthesis silver nanoparticles using Sargassum polycystum and its antioxidant and cytotoxic effects: an in vitro analysis. Materials Letters,189:196-200.
19. Kumar P, Selvi SS, Praba A, et al (2012). Antibacterial activity and in-vitro cytotoxicity assay against brine shrimp using silver nanoparticles synthesized from Sargassum ilicifolium. Dig J Nanomater Biostruct,7(4):1447-1455.
20. Manikandan R, Anjali R, Beulaja M, et al (2019). Synthesis, characterization, anti-proliferative and wound healing activities of silver nanoparticles synthesized from Caulerpa scalpelliformis. Process Biochemistry,79:135-141.
21. Asha KS, Johnson M, Chandra KP, et al (2015). Extracellular synthesis of silver nanoparticles from a marine alga, Sargassum polycystum C. World J Pharm Pharm Sci,4(9):1388-1400.
22. Kavipriya B, Balaji K, Selvarani S, et al (2017). COUROUPITA SP. Enhanced silver nanoparticles as potent anticancer agent. IJCSET ,3(1):33-36.
23. Wu T, Chen W, Kong D, et al (2015). miR-25 targets the modulator of apoptosis 1 gene in lung cancer. Carcinogenesis,36(8):925-35.
24. Tuo Y, Li X, Luo J (2015). Long noncoding RNA UCA1 modulates breast cancer cell growth and apoptosis through decreasing tumor suppressive miR-143. Eur Rev Med Pharmacol Sci,19(18):3403-11.
25. Li D, Hu J, Song H, et al (2017). miR-143-3p targeting LIM domain kinase 1 suppresses the progression of triple-negative breast cancer cells. Am j Transl Res,9(5):2276-2285.
26. Xu N, Fan X, Yan X, et al (2004). Screening marine algae from China for their antitumor activities. J App Phycol,16:451-456.
27. Ghaffari S, Torabi-Rahvar M, Aghayan S, et al (2021). Optimizing interleukin-2 concentration, seeding density and bead-to-cell ratio of T-cell expansion for adoptive immunotherapy. BMC immunol,,22(1):43.
28. Li X, Zhou DY, Li FT, et al (2022). Saringosterol Acetate Isolated from Sargassum fusiformis Induces Mitochondrial‐Mediated Apoptosis in MCF‐7 Breast Cancer Cells. Chem Biodivers,19(3):e202100848.
29. Obeidi N, Mousavi MJ, Hosseinpouri A, et al (2020). Anticoagulative Effect of Two Species of Brown Algae; Sargassum Angustifolium and Cystoseira Indica. Res Mol Med,8(3):125-132.
30. Vaseghi G, Sharifi M, Dana N, et al (2018). Cytotoxicity of Sargassum angustifolium partitions against breast and cervical cancer cell lines. Adv Biomedi Res, 7:43.
31. Khanavi M, Gheidarloo R, Sadati N, et al (2012). Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line. Pharmacogn Mag,8(29):60-4.
32. Obeidi N, Babolei MD, Khamisipour G, et al (2023). The Effect of Sargassum Angustifolium-derived AgNPs on Apoptosis-associated Genes in Acute Lymphoblastic Leukemia Cells. Asian Pacific Journal of Cancer Prevention. Asian Pac J Cancer Prev,24(8):2691-2696.
33. Hartmann JU, Bräuer-Hartmann D, Kardosova M, et al (2018). MicroRNA-143 targets ERK5 in granulopoiesis and predicts outcome of patients with acute myeloid leukemia. Cell death dis,9(8):814.
34. Amiri BS, Sabernia N, Abouali B, Amini P, Rezaeeyan H (2023). Evaluation of MicroRNA as Minimal Residual Disease in Leukemia: Diagnostic and Prognostic Approach: A Review. Iran J Public Health,52(12):2541-2553.
2. Vijayakrishnan J, Studd J, Broderick P, et al (2018). Genome-wide association study identifies susceptibility loci for B-cell childhood acute lymphoblastic leukemia. Nat Commun,9(1):1340-1348.
3. Bhojwani D, Yang JJ, Pui CH (2015). Biology of childhood acute lymphoblastic leukemia. Pediatr Clin North Am,62(1):47-60.
4. Belver L, Ferrando A (2016). The genetics and mechanisms of T cell acute lymphoblastic leukaemia. Nat Rev Cancer, 16(8):494-507.
5. Coniglio SJ, Blackman JA (1995). Developmental outcome of childhood leukemia. Topics Early Child Spec Educ,15(1):19-31.
6. Clapé C, Fritz V, Henriquet C, et al (2009). miR-143 interferes with ERK5 signaling, and abrogates prostate cancer progression in mice. PLoS One, 4(10):e7542.
7. Vannini I, Fanini F, Fabbri M (2018). Emerging roles of microRNAs in can-cer. Curr Opin Genet Dev,48:128-133.
8. Shokri G, Kouhkan F, Nojehdehi S, et al (2019). Simultaneous regulation of miR-451 and miR-191 led to erythroid fate decision of mouse embryonic stem cell. Iran J Basic Med Sci,22(4):432-438.
9. Papagiannopoulos CI, Theodoroula NF, Vizirianakis IS (2021). miR-16-5p promotes erythroid maturation of erythroleukemia cells by regulating ribosome biogenesis. Pharmaceuticals (Basel),14(2):137.
10. Liu L, Yu X, Guo X, et al (2012). miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2. Mol Med Rep, 5(3):753-60.
11. Parsa N (2012). Cellular and molecular basis of cancer in humans. Cell & Tissue Journal ,2(4):365-76.
12. Xu Y, Yang J, Li X (2016). MicroRNA-25 promotes T-cell acute lymphoblastic leukemia cell proliferation and invasion by directly targeting EphA8. Int J Clin Exp Pathol,9(5):5292-8.
13. Akao Y, Nakagawa Y, Iio A, Naoe T (2009). Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells. Leuk Res, 33(11):1530-8.
14. Pugazhendhi A, Prabhu R, Muruganantham K, et al (2019). Anticancer, antimicrobial and photocatalytic activities of green synthesized magnesium oxide nanoparticles (MgONPs) using aqueous extract of Sargassum wightii. J Photochem Photobiol B,190:86-97.
15. Kumar P, Senthamil Selvi S, Lakshmi Prabha A, et al (2012). Synthesis of silver nanoparticles from Sargassum tenerrimum and screening phytochemicals for its antibacterial activity. Nano Biomed Eng,4(1):12-16.
16. Ghaemi M, Gholamipoor S (2017). Controllable synthesis and characterization of silver nanoparticles using Sargassum angostifolium. Iran J Chem Chem Eng, 36(1):1-10
17. Gu Y, Liang Y, Gong J, et al (2012). Suitable internal control microRNA genes for measuring miRNA abundance in pig milk during different lactation periods. Genet and Mol Res,11(3):2506-12.
18. Palanisamy S, Rajasekar P, Vijayaprasath G, et al (2017). A green route to synthesis silver nanoparticles using Sargassum polycystum and its antioxidant and cytotoxic effects: an in vitro analysis. Materials Letters,189:196-200.
19. Kumar P, Selvi SS, Praba A, et al (2012). Antibacterial activity and in-vitro cytotoxicity assay against brine shrimp using silver nanoparticles synthesized from Sargassum ilicifolium. Dig J Nanomater Biostruct,7(4):1447-1455.
20. Manikandan R, Anjali R, Beulaja M, et al (2019). Synthesis, characterization, anti-proliferative and wound healing activities of silver nanoparticles synthesized from Caulerpa scalpelliformis. Process Biochemistry,79:135-141.
21. Asha KS, Johnson M, Chandra KP, et al (2015). Extracellular synthesis of silver nanoparticles from a marine alga, Sargassum polycystum C. World J Pharm Pharm Sci,4(9):1388-1400.
22. Kavipriya B, Balaji K, Selvarani S, et al (2017). COUROUPITA SP. Enhanced silver nanoparticles as potent anticancer agent. IJCSET ,3(1):33-36.
23. Wu T, Chen W, Kong D, et al (2015). miR-25 targets the modulator of apoptosis 1 gene in lung cancer. Carcinogenesis,36(8):925-35.
24. Tuo Y, Li X, Luo J (2015). Long noncoding RNA UCA1 modulates breast cancer cell growth and apoptosis through decreasing tumor suppressive miR-143. Eur Rev Med Pharmacol Sci,19(18):3403-11.
25. Li D, Hu J, Song H, et al (2017). miR-143-3p targeting LIM domain kinase 1 suppresses the progression of triple-negative breast cancer cells. Am j Transl Res,9(5):2276-2285.
26. Xu N, Fan X, Yan X, et al (2004). Screening marine algae from China for their antitumor activities. J App Phycol,16:451-456.
27. Ghaffari S, Torabi-Rahvar M, Aghayan S, et al (2021). Optimizing interleukin-2 concentration, seeding density and bead-to-cell ratio of T-cell expansion for adoptive immunotherapy. BMC immunol,,22(1):43.
28. Li X, Zhou DY, Li FT, et al (2022). Saringosterol Acetate Isolated from Sargassum fusiformis Induces Mitochondrial‐Mediated Apoptosis in MCF‐7 Breast Cancer Cells. Chem Biodivers,19(3):e202100848.
29. Obeidi N, Mousavi MJ, Hosseinpouri A, et al (2020). Anticoagulative Effect of Two Species of Brown Algae; Sargassum Angustifolium and Cystoseira Indica. Res Mol Med,8(3):125-132.
30. Vaseghi G, Sharifi M, Dana N, et al (2018). Cytotoxicity of Sargassum angustifolium partitions against breast and cervical cancer cell lines. Adv Biomedi Res, 7:43.
31. Khanavi M, Gheidarloo R, Sadati N, et al (2012). Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line. Pharmacogn Mag,8(29):60-4.
32. Obeidi N, Babolei MD, Khamisipour G, et al (2023). The Effect of Sargassum Angustifolium-derived AgNPs on Apoptosis-associated Genes in Acute Lymphoblastic Leukemia Cells. Asian Pacific Journal of Cancer Prevention. Asian Pac J Cancer Prev,24(8):2691-2696.
33. Hartmann JU, Bräuer-Hartmann D, Kardosova M, et al (2018). MicroRNA-143 targets ERK5 in granulopoiesis and predicts outcome of patients with acute myeloid leukemia. Cell death dis,9(8):814.
34. Amiri BS, Sabernia N, Abouali B, Amini P, Rezaeeyan H (2023). Evaluation of MicroRNA as Minimal Residual Disease in Leukemia: Diagnostic and Prognostic Approach: A Review. Iran J Public Health,52(12):2541-2553.
| Files | ||
| Issue | Vol 54 No 8 (2025) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v54i8.19586 | |
| Keywords | ||
| Acute lymphoblastic leukemia (ALL) Nanoparticles Silver | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Valipour S, Obeidi N, Khamisipour G, Mohammadi SA, Ghaemi M, Farhangdoost F, Malekhayaty H, Darzipour M. Silver Nanoparticles Synthesised Using Sargassum Angustifolium Can Effect on MiR-25 and MiR-143 Expression in Acute Lymphoblastic Leukemia. Iran J Public Health. 2025;54(8):1776-1785.
