Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 54 8 2025 09 02 1776 1785 Sepideh Valipour Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Narges Obeidi 1. Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran 2. The Persian Gulf Marine Biotechnology Research Center, Bushehr, Iran Gholamreza Khamisipour Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Seyed Amin Mohammadi Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Maryam Ghaemi Iranian National Institute for Oceanography and Atmospheric Science, Bushehr, Iran Farzad Farhangdoost Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Hamideh Malekhayaty Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran Maedeh Darzipour Department of Hematology, School of Para Medicine, Bushehr University of Medical Sciences, Bushehr, Iran 2024 03 08 2025 04 07 Background: One of the treatments of acute lymphoblastic leukemia (ALL) is chemotherapy but it can destroy other normal cells as well as cancer cells, which can lead to infection and bleeding. The widespread consumption of nanoparticles synthesized biogenically by seaweed is because of their easy accessibility and usefulness. We aimed to investigate silver nanoparticles synthesised using Sargassum on the expression of miR-25 and 143 in Jurkat cell line. Methods: In an interventional study in 2019, Bushehr, Iran, following culture of the Jurkat cell line with 95% survival rate, we applied different drug concentrations to 20,000 cell lines and control cells separately to determine maximum cell growth inhibition and IC50. After treatment for 48 h, RNA extraction was performed, and Real Time PCR was used to evaluate miR-25 and 143 expressions. Results: MiR-25 expression in the groups that treated with the maximum dose and IC50 of silver nanoparticles and algae extract was decline but did not differ significantly from that of the control group. Conversely, miR-143 showed a remarkable decline in both treatment groups (P<0.0001). After treating PBMC with nanoparticles the expression level of miR-25 and miR-143were not significantly different. Conclusion: The level of miR-25 and 143 expressions were decrease in the IC50 dosage of silver nanoparticles and algae extract produced. The level of miR-25 expression decreased more in the treated Jurkat cell line than the level of miR-143 expression in normal lymphocytes. Given the oncogenicity of miR-25 in the Jurkat cell line, it is suggested that decreasing level of this microRNA can help to apoptosis of leukemic cells. https://ijph.tums.ac.ir/index.php/ijph/article/view/34855 https://ijph.tums.ac.ir/index.php/ijph/article/download/34855/8632