Metagenomic Next-Generation Sequencing for the Diagnosis of Epstein-Barr Virus Pleurisy: A Case Report
-
Hui Lv
,
JIn Liu
,
Ruoyu Zhang
Abstract
Chronic active Epstein-Barr virus infection (CAEBV) progresses rapidly in the later stage and has a poor prognosis. The treatments of CAEBV have no unified standard and a bad effect. Only few person could cure. It is important to diagnosis early. Metagenomic next-generation sequencing (mNGS) offers an effective means for the diagnosis of difficult, critical and rare pathogenic microbial infections. Here, we report a case of viral pleurisy caused by CAEBV identified by mNGS in an 88-year-old Chinese male patient.
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24. Kim D, Song L, Breitwieser F, et al (2016). Centrifuge: rapid and sensitive classifica-tion of metagenomic sequences. Genome Res, 26(12):1721-1729.
2. Sakai M, Togitani K, Tsukuda T, et al (2015). [Young adult onset systemic Epstein-Barr virus-positive T-cell lymphoproliferative disorders of childhood]. Rinsho Ketsueki, 56(5): 501-505.
3. Yoshioka K, Fukushima H, Ishii N, et al (2013). A case of chronic active Epstein-Barr virus infection mimicking adult-onset Still’s disease. Mod Rheumatol, 23(1): 162-166.
4. Fujiwara S,Kimura H,Imadome K, et al (2014). Current research on chronic active Epstein-Barr virus infection in Japan. Pe-diatr Int, 56(2):159-166.
5. Wilson MR, Naccache SN, Samayoa E, et al (2014). Actionable diagnosis of neurolep-tospirosis by next-generation sequencing. N Engl J Med, 370(25): 2408-2417.
6. Wilson MR, Suan D, Duggins A, et al (2017). A novel cause of chronic viral meningoencephalitis: Cache Valley virus. Ann Neurol, 82(1): 105-114.
7. Wilson MR, O’Donovan BD, Gelfand JM, et al (2018). Chronic meningitis investi-gated via metagenomic next-generation sequencing. JAMA Neurol, 75(8): 947-955.
8. Wilson MR, Zimmermann LL, Crawford ED, et al (2017). Acute West Nile virus meningoencephalitis diagnosed via meta-genomic deep sequencing of cerebrospi-nal fluid in a renal transplant patient. Am J Transplant, 17(3): 803-808.
9. Murkey JA, Chew KW, Carlson M, et al (2017). Hepatitis E virus–associated me-ningoencephalitis in a lung transplant re-cipient diagnosed by clinical metagenomic sequencing. Open Forum Infect Dis, 4(3):ofx121.
10. Chiu CY, Coffey LL, Murkey J, et al (2017). Diagnosis of fatal human case of St. Louis Encephalitis virus infection by metagenomic sequencing, California, 2016. Emerg Infect Dis, 23(10): 1964-1968.
11. Gu W, Crawford ED, O’Donovan BD, et al (2016). Depletion of abundant sequences by hybridization (DASH): using Cas9 to remove unwanted high-abundance spe-cies in sequencing libraries and molecular counting applications. Genome Biol, 17:41.
12. Langelier C, Zinter MS, Kalantar K, et al (2018). Metagenomic sequencing detects respiratory pathogens in hematopoietic cellular transplant patients. Am J Respir Crit Care Med, 197(4): 524-528.
13. Pendleton KM, Erb-Downward JR, Bao Y, et al (2017). Rapid pathogen identification in bacterial pneumonia using real-time metagenomics. Am J Respir Crit Care Med, 196(12): 1610-1612.
14. Kujiraoka M, Kuroda M, Asai K, et al (2017). Comprehensive diagnosis of bac-terial infection associated with acute chol-ecystitis using metagenomic approach. Front Microbiol, 8:685.
15. Pan W, Ngo TTM, Camunas-Soler J, et al (2017). Simultaneously monitoring im-mune response and microbial infections during pregnancy through plasma cfRNA sequencing. Clinical Chemistry, 63(11): 1695-1704.
16. Abril MK, Barnett AS, Wegermann K, et al (2016). Diagnosis of Capnocytophaga cani-morsus sepsis by whole-genome next-generation sequencing. Open Forum Infect Dis, 3(3): ofw144.
17. Gosiewski T, Ludwig-Galezowska AH, Huminska K, et al (2017). Com-prehensive detection and identification of bacterial DNA in the blood of patients with sepsis and healthy volunteers using next-generation sequencing method—the observation of DNAemia. Eur J Clin Mi-crobiol Infect Dis, 36(2): 329-336.
18. Doan T, Acharya NR, Pinsky BA, et al (2017). Metagenomic DNA sequencing for the diagnosis of intraocular infec-tions. Ophthalmology, 124(8): 1247-1248.
19. Chiu CY (2013). Viral pathogen discovery. Curr Opin Microbiol, 16(4): 468-478.
20. Gu Wei, Miller Steve, Chiu CY (2019). Clini-cal metagenomic next-generation se-quencing for pathogen detection. Annu Rev Pathol, 14:319-338.
21. Lefterova MI, Suarez CJ, Banaei N, et al (2015). Next-generation sequencing for infectious disease diagnosis and man-agement: a report of the association for molecular pathology. J Mol Diagn, 17(6):623-634.
22. Chiu CY, Miller SA (2019). Clinical Meta-genomics. Nat Rev Genet, 20(6): 341-355.
23. Flygare S, Simmon K, Miller C, et al (2016). Taxonomer: an interactive metagenomics analysis portal for universal pathogen de-tection and host mRNA expression pro-filing. Genome Biol, 17:111.
24. Kim D, Song L, Breitwieser F, et al (2016). Centrifuge: rapid and sensitive classifica-tion of metagenomic sequences. Genome Res, 26(12):1721-1729.
| Files | ||
| Issue | Vol 50 No 11 (2021) | |
| Section | Case Report(s) | |
| DOI | https://doi.org/10.18502/ijph.v50i11.7589 | |
| Keywords | ||
| Metagenomic next-generation sequencing Chronic active Epstein-Barr virus infection Viral pleurisy | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Lv H, Liu J, Zhang R. Metagenomic Next-Generation Sequencing for the Diagnosis of Epstein-Barr Virus Pleurisy: A Case Report. Iran J Public Health. 2021;50(11):2326-2331.
