Pyrrolidinedithiocarbamic Acid Ammonium Salt Inhibits Apop-tosis and Phenotypic Transformation of Co-Culture of Myeloma Cells and Renal Tubular Epithelial Cells by Reducing the Secretion of Light Chain Protein

  • Xiaoyan YU Department of Nephrology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China
  • Jie BAO Mail Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China
  • Xinyu CUI Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China
  • Fengxia DU Department of Pathogenic Biology, Qiqihar Medical University, Qiqihar161006, P.R. China
  • Yuefei WANG Department of Physiology, Qiqihar Medical University, Qiqihar161006, P.R. China
  • Lili BI Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China
  • Jun SUN Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China
  • Ling LI Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China
Keywords:
Myeloma nephropathy, Renal tubular epithelial cells, Light chain protein

Abstract

Background: We investigate the effects of NFκB inhibitor pyrrolidinedithiocarbamic acid ammonium salt (PDTC) on the viability, apoptosis and cell phenotype of HK-2 cells in the co-culture system of myeloma cells in renal tubular epithelial cells.

Methods: This study was performed in Qiqihar Medical University, Qiqihar, China from Jun 2018 to Jan 2019. RPMI-8226 cells and HK-2 cells were inoculated in the co-culture chamber and cultured to establish the co-culture system. An immunoturbidimetric assay was performed to detect κ light chain and λ light chain in RPMI-8226 cells. The effect of PDTC on the secretion of κ light chain and λ light chain of RPMI-8226 cells was detected by immunoturbidimetry and the ratio was calculated.

Results: PDTC significantly increased the viability of HK-2 cells. PDTC reduced the apoptosis of renal tubular epithelial cells. After PDTC treatment, the expression of cell surface marker E-cadherin decreased, and the expression of α-SMA increased, which induced the renal interstitial fibrosis. The secretion of κ light chain and λ light chain of RPMI-8226 cells was significantly decreased after the addition of PDTC, but the ratio was not changed.

Conclusion: PDTC can inhibit the cell activity, promote apoptosis, and reduce the secretion of secretion of κ light chain and λ light chain through inhibiting the NF-κB pathway activation of myeloma cell RPMI-8226.

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Published
2020-10-27
How to Cite
1.
YU X, BAO J, CUI X, DU F, WANG Y, BI L, SUN J, LI L. Pyrrolidinedithiocarbamic Acid Ammonium Salt Inhibits Apop-tosis and Phenotypic Transformation of Co-Culture of Myeloma Cells and Renal Tubular Epithelial Cells by Reducing the Secretion of Light Chain Protein. Iran J Public Health. 49(11):2078-2086.
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Original Article(s)