Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 49 11 2020 10 27 2078 2086 Xiaoyan YU Department of Nephrology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China Jie BAO Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China Xinyu CUI Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China Fengxia DU Department of Pathogenic Biology, Qiqihar Medical University, Qiqihar161006, P.R. China Yuefei WANG Department of Physiology, Qiqihar Medical University, Qiqihar161006, P.R. China Lili BI Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China Jun SUN Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China Ling LI Department of Hematopathology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar161000, P.R. China 2020 04 24 2020 10 27 Background: We investigate the effects of NFκB inhibitor pyrrolidinedithiocarbamic acid ammonium salt (PDTC) on the viability, apoptosis and cell phenotype of HK-2 cells in the co-culture system of myeloma cells in renal tubular epithelial cells. Methods: This study was performed in Qiqihar Medical University, Qiqihar, China from Jun 2018 to Jan 2019. RPMI-8226 cells and HK-2 cells were inoculated in the co-culture chamber and cultured to establish the co-culture system. An immunoturbidimetric assay was performed to detect κ light chain and λ light chain in RPMI-8226 cells. The effect of PDTC on the secretion of κ light chain and λ light chain of RPMI-8226 cells was detected by immunoturbidimetry and the ratio was calculated. Results: PDTC significantly increased the viability of HK-2 cells. PDTC reduced the apoptosis of renal tubular epithelial cells. After PDTC treatment, the expression of cell surface marker E-cadherin decreased, and the expression of α-SMA increased, which induced the renal interstitial fibrosis. The secretion of κ light chain and λ light chain of RPMI-8226 cells was significantly decreased after the addition of PDTC, but the ratio was not changed. Conclusion: PDTC can inhibit the cell activity, promote apoptosis, and reduce the secretion of secretion of κ light chain and λ light chain through inhibiting the NF-κB pathway activation of myeloma cell RPMI-8226. https://ijph.tums.ac.ir/index.php/ijph/article/view/20484 https://ijph.tums.ac.ir/index.php/ijph/article/download/20484/7034