Expressions of Fib, IL-12 in Serum of Neonatal Necrotizing Enterocolitis and Their Correlation with Clinicopathological Features
Background: To investigate the expressions of fibrinogen (Fib) and Interleukin-12 (IL-12) in serum of neonatal necrotizing enterocolitis (NEC), and to analyze the correlation between the two and their relationship with clinicopathological features.
Methods: Forty two children with NEC treated in Xuzhou Children’s Hospital, Xuzhou Medical University Xuzhou, China from 2016-2019 were selected as an observation group and 40 children who underwent physical examination at the same period as a control group. The expression levels of Fib and IL-12 in the serum of two groups were detected by ELISA. The correlation between Fib and IL-12 in the observation group and the correlation among the expressions of Fib, IL-12, the clinicopathological features and common examination indexes of the children with NEC were investigated by Pearson correlation analysis.
Results: The levels of Fib and IL-12 in the serum of the children in observation group were significantly higher than those in the control group were (P<0.05). There was a significant positive correlation between the levels of Fib and IL-12 in the serum of the children in observation group (P<0.05). The expression levels of Fib, IL-12 were not significantly correlated with sex and age of NEC children, but correlated with vomiting, diarrhea, bloody stool and bradycardia in NEC children (P<0.05). Fib and IL-12 were positively correlated with erythrocyte level (P<0.05) and negatively correlated with platelet level.
Conclusion: The expressions of Fib and IL-12 in the serum of NEC children can objectively predict the severity of NEC.
2. Hsueh W, Caplan MS, Qu XW, Tan XD, De Plaen IG, Gonzalez-Crussi F (2003). Neonatal necrotizing enterocolitis: clinical considerations and pathogenetic con-cepts. Pediatr Dev Pathol, 6: 6-23.
3. Kalogeris T, Baines CP, Krenz M, Korthuis RJ (2012). Cell biology of ische-mia/reperfusion injury. Int Rev Cell Mol Bi-ol, 298: 229-317.
4. NEU J, WALKER WA (2011). Necrotizing enterocolitis. N Engl J Med, 364: 255-264.
5. Hall Nj, Eatone S, Pierrop A (2013). Royal Australasia of Surgeons Guest Lec-ture.Necrotizing enterocoitis: prevention, treatment, and outcome. J Pediatr Surg, 48: 2359-2367.
6. Laukaityte A (2013). Neonatal necrotising enterocolitis-Fact sheet. J Neonatal Nursing, 19: 54-57.
7. Jin YT, Duan Y, Deng XK, Lin J (2019). Prevention of necrotizing enterocolitis in premature infants - an updated review. World J Clin Pediatr, 8: 23-32.
8. Drucker NA, McCulloh CJ, Li B, Pierro A, Besner GE, Markel TA (2017). Stem cell therapy in necrotizing enterocolitis: Cur-rent state and future directions. Semin Pe-diatr Surg, 27: 57-64.
9. Thyoka M, de Coppi P, Eaton S, et al (2012). Advanced necrotizing enterocolitis part 1:mortality. Eur J Pediatr Surg, 22: 8-12.
10. Amin SC, Pappas C, Iyengar H, Maheshwari A (2013). Short bowel syndrome in the NICU. Clin Perinatol, 40: 53-68.
11. Kume K, Yamasaki M, Tashiro M, Yoshi-kawa I, Otsuki M (2007). Activations of coagulation and fibrinolysis secondary to bowel inflammation in patients with ul-cerative colitis. Inter Med, 46: 1323-1329.
12. Christensen RD, Baer VL, Henry E, Snow GL, Butler A, Sola-Visner MC (2015). Thrombocytopenia in Small-for-Gestational-Age Infants. Pediatrics, 136: e361-e370.
13. Egwuagu CE, Yu CR, Sun L, Wang R (2015). Interleukin 35: Critical regulator of immunity and lymphocyte-mediated dis-eases. Cytokine Growth Factor Rev, 26: 587-593.
14. Murphy CA, Langrish CL, Chen Y, et al (2003). Divergent Pro- and Antiinflam-matory Roles for IL-23 and IL-12 in Joint Autoimmune Inflammation. J Exp Med, 198: 1951-1957.
15. Eaton S (2017). Necrotizing enterocolitis symposium: Epidemiology and early di-agnosis. J Pediatr Surg, 52: 223-225.
16. Vieten D, Corfield A, Carroll D, Ramani P, Spicer R (2004). Impaired mucosal regen-eration in neonatal necrotising enterocol-itis. Pediatr Surg Int, 21: 153-160.
17. Heida FH, Harmsen HJ, Timmer A, Kooi EM, Bos AF, Hulscher JB (2016). Identi-fication of bacterial invasion in necrotiz-ing enterocolitis specimens using fluores-cent in situ hybridization. J Perinatol, 37: 67-72.
18. Weber P, Husemann S, Vielhaber H, Zim-mer KP, Nowak-Göttl U (1999). Coagu-lation and fibrinolysis in children, adoles-cents, and young adults with inflammato-ry bowel disease. J Pediatr Castroenterol Nutr, 28: 418-422.
19. Nielsen OH, Kiman I, Rudiger N, Hendel J, Vainer B (2003). Upregulation of interleu-kin-12 and-17 in active inflammatory bowel disease. Scand J Gastroenterol, 38: 180-185.
20. Sugimoto K, Ogawa A, Shimomura Y, Na-gahama K, Mizoguchi A, Bhan AK (2007). Inducible IL-12-producing B cells regulate Th2-mediated intestinal inflam-mation. Gastroenterology, 133: 124-136.
21. Hino A, Kweon MN, Fujihashi K, McGhee JR, Kiyono H (2004). Pathological role of large intestinal IL-12p40 for the induction of Th2-type allergic diarrhea. Am J Pathol, 164: 1327-1335.
22. Dong X, Ye X, Chen X, Chen T, Xie S, Li Q, Lin X, Huang Z (2013). Intestinal and peripheral fibrinogen-like protein 2 ex-pression in inflammatory bowel disease. Dig Dis Sci, 59: 769-777.
23. Ledbetter DJ, Juul SE (2000). Erythropoietin and the incidence of necrotizing in in-fants with very low birth weight. J Pediatr Surg, 35: 178-181.
24. Robinson JR, Rellinger EJ, Hatch LD, Weitkamp JH, Speck KE, Danko M, Blakely ML (2016). Surgical necrotizing enterocolitis. Semin Perinatol, 41: 70-79.
25. Ververidis M, Kiely EM, Spitz L, Drake DP, Eaton S, Pierro A (2001). The clinical sig-nificance of thrombocytopenia in neo-nates with necrotizing enterocolitis. J Pedi-atr Surg, 36: 799-803.
|Issue||Vol 49 No 8 (2020)|
|Interleukin-12; Neonatal necrotizing enterocolitis; Clinicopathological features; Erythrocyte; Platelet|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|