Single Nucleotide Polymorphisms of FCRL3 in Iranian Patients with Behcet’s Disease
Abstract
Background: Both genetic and environmental factors influence, susceptibility to autoimmune disorders including Behcet’s disease (BD). FCRL3 (Fc receptor like 3 genes), a novel immunoregulatory gene, has recently been reported as a new promising candidate gene for general autoimmunity. This study was conducted to explore the potential association of FCRL3 polymorphisms with BD.
Methods: This study was conducted from 2010 to 2015 in Tehran University of Medical Sciences, Tehran, Iran. Four single-nucleotide polymorphisms of FCRL3 (rs7528684, rs11264799, rs945635, and rs3761959) were genotyped in 220 patients and 220 healthy controls. Typing of the polymorphisms in this case-control study was carried out using polymerase chain reaction-restriction fragment length polymorphism analysis.
Results: Analysis of the alleles revealed a significantly lower frequency of the A allele at the -169 site (rs7528684) in BD patients compared with that in controls (P=0.000, 66.4% versus 82%, χ2= 30.23). Moreover, a significant lower frequency of AA genotype and higher frequency of GG genotype was recorded for rs7528684. There was also relationship between posterior uveitis as a clinical sign of disease and polymorphism of allele A at the -169 site (P=0.015).
Conclusion: This study revealed a significant difference in both allele and genotype frequency at position -169 of FCRL3 gene between Iranian patients with BD and normal subjects. These data suggest FCRL3 gene polymorphisms might be the autoimmunity risk factor for BD.
2. Morton LT, Situnayake D, Wallace GR (2016). Genetics of Behçet's disease. Curr Opin Rheumatol, 28(1):39-44.
3. Yu H, Zheng M, Zhang L et al (2017). Iden-tification of susceptibility SNPs in IL10 and IL23R-IL12RB2 for Behçet's disease in Han Chinese. J Allergy Clin Immunol, 139(2):621-7.
4. Takeuchi M, Mizuki N, Meguro A et al (2017). Dense genotyping of immune-related loci implicates host responses to microbial exposure in Behçet's disease susceptibility. Nat Genet, 49(3):438-43.
5. Lee YJ, Horie Y, Wallace GR et al (2013). Genome-wide association study identifies GIMAP as a novel susceptibility locus for Behcet's disease. Ann Rheum Dis, 72(9):1510-6.
6. Abdolmohammadi R, Bonyadi M (2017). Polymorphisms of Promoter Region of TNF-α Gene in Iranian Azeri Turkish Pa-tients with Behçet's Disease. J Korean Med Sci, 32(1):33-7.
7. Gabrielsen IS, Amundsen SS, Helgeland H et al (2016).Genetic risk variants for auto-immune diseases that influence gene ex-pression in thymus. Hum Mol Genet, 25(14):3117-24.
8. Davis RS, Wang YH, Kubagawa H, Cooper MD (2001). Identification of a family of Fc receptor homologs with preferential B cell expression. Proc Natl Acad Sci U S A, 98(17):9772-7.
9. Yang Y, Su X, Zhang K, Zhou R (2013). The Fc receptor-like 3 gene polymor-phisms and susceptibility to autoimmune diseases: an updated meta-analysis. Auto-immunity, 46(8):547-58.
10. Lin X, Zhang Y, Chen Q (2016). FCRL3 gene polymorphisms as risk factors for rheumatoid arthritis. Hum Immunol, 77(2):223-9.
11. Yuan M, Wei L, Zhou R et al (2016). Four FCRL3 Gene Polymorphisms (FCRL3_3, _5, _6, _8) Confer Suscepti-bility to Multiple Sclerosis: Results from a Case-Control Study. Mol Neurobiol, 53(3):2029-35.
12. Gu Z, Hong SL, Ke X et al (2015). FCRL3 gene polymorphisms confer autoim-munity risk for allergic rhinitis in a Chi-nese Han population. PLoS One, 10(1):e0116419.
13. Fang Y, Li Y, Zeng J et al (2016). Genetic association of Fc receptor-like glycopro-tein with susceptibility to Graves' disease in a Chinese Han population. Immunobiolo-gy, 221(1):56-62.
14. Li K, Zhao M, Hou S et al (2008). Associa-tion between polymorphisms of FCRL3, a non-HLA gene, and Behçet's disease in a Chinese population with ophthalmic manifestations. Mol Vis, 14:2136-42.
15. International Team for the Revision of the International Criteria for Behçet's Disease (ITR-ICBD) (2014). The International Criteria for Behçet's Disease (ICBD): a collaborative study of 27 countries on the sensitivity and specificity of the new crite-ria. J Eur Acad Dermatol Venereol, 28(3):338-47.
16. Zhang H, Zhang Z, Li G et al (2015). Asso-ciation of FCRL3 genetic polymorphisms with endometriosis-related infertility risk: An independent study in Han Chinese. Medicine (Baltimore), 94(35):e1168.
17. Inoue N, Watanabe M, Yamada H et al (2012). Associations between autoim-mune thyroid disease prognosis and functional polymorphisms of susceptibil-ity genes, CTLA4, PTPN22, CD40, FCRL3, and ZFAT, previously revealed in genome-wide association studies. J Clin Immunol, 32(6):1243-52.
18. Kochi Y, Yamada R, Suzuki A et al (2005). A functional variant in FCRL3, encoding Fc receptor-like 3, is associated with rheuma-toid arthritis and several autoimmunities. Nat Genet, 37(5):478-85.
19. Kochi Y, Myouzen K, Yamada R et al (2009). FCRL3, an autoimmune suscepti-bility gene, has inhibitory potential on B-cell receptor-mediated signaling. J Immu-nol, 183(9):5502-10.
20. Swainson LA, Mold JE, Bajpai UD, McCu-ne JM (2010). Expression of the auto-immune susceptibility gene FcRL3 on human regulatory T cells is associated with dysfunction and high levels of pro-grammed cell death-1. J Immunol, 184(7):3639-47.
21. Owen CJ, Kelly H, Eden JA et al (2007). Analysis of the Fc receptor-like-3 (FCRL3) locus in Caucasians with auto-immune disorders suggests a complex pattern of disease association. J Clin Endo-crinol Metab, 92(3):1106-11.
22. Chistiakov DA, Chistiakov AP (2007). Is FCRL3 a new general autoimmunity gene? Hum Immunol, 68(5):375-83.
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| Issue | Vol 48 No 6 (2019) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v48i6.2926 | |
| Keywords | ||
| Behcet disease Fc receptor like 3 (FCRL3 Polymorphism Autoimmunity | ||
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