Larvicidal Activity of Brusatol Isolated from Brucea javanica (L) Merr on Culex quinquefasciatus
Background: Vector control is still emphasized on the using of chemical insecticide which can cause death of non-target organisms, pollution and vector resistance. Therefore, natural insecticides/larvicides are an alternative to using chemical insecticides to control the mosquito vector.
Methods: Brusatol was isolated from the seeds of Makassar Fruit (Brucea javanica L. Merr). Culex quinquefasciatus larvae were divided into 3 groups, i.e. 6 testing groups and one negative and positive control group. In the negative control group, the larvae were treated with 100 ml aquadest and positive control was treated with temephos 1 ppm. After 24 hours, dead larvae were calculated and the percentage of death was determined. The dead larvae were then examined for morphological changes using a light microscope.
Results: The higher of the concentration level of brusatol, the higher number of the death of Cx. quinquefasciatus larvae (P<0.05). The value of brusatol Lethal Concentration 50 to larvae Cx. quinquefasciatus was 0.010 ± 0.122 and value of brusatol Lethal Concentration 90 to larvae Cx. quinquefasciatus was 0.654 ± 0.081 ppm. The higher the concentration of brusatol, the morphological damage of Cx. quinquefasciatus larvae was getting worse and widespread to cause damage to the digestive tract and cuticle.
Conclusion: Brusatol isolated from the seed of B. javanica (L) Merr have larvicidal activity to the Cx. quinquefasciatus which is potential to be natural larvicide.
2. Isman MB (2006). Botanical insecticides, de-terrents, and repellents in modern agricul-ture and an increasingly regulated world. Annu Rev Entomol, 51:45–66.
3. Isman MB (2008). Botanical insecticides: for richer, for poorer. Pest Manag Sci, 64 (1):8–11.
4. Takeya K, Kim I, Hitotsuyanagi Y, Hasuda T (2006). Antitumour quassinoid from Brucea javanica and SAR of Brusatol. Planta Med, 72:20–4.
5. Govindachari T, Krishna KGN, Gopala-krishnan G, Suresh G, Wesley S, Seelatha T (2001). Insect antifeedant and growth regulating activities of quassinoids from Samadera indica. Fitoterapia, 72:568–71.
6. Chen YY, Pan Q, Li D, Liu J, Wen Y, Huang Y, Lu F (2011). New pregnane glycosides from Brucea javanica and their antifeedant activity. Chem Biodivers, 31:460–6.
7. Zhang L, Feng X, Ma D, Yang J, Jiang H, Zhang Y, He W (2013). Brusatol isolated from Brucea javanica (L) Merr induces apoptotic death of insect cell lines. Pestic Biochem Physiol, 107 (1):18–24.
8. Sutiningsih D, Nurjazuli (2017). Effect of brusatol biolarvicide administration on behavioral response of Aedes aegypti and its toxicity on Vero cells. J Biol Sci, 17:127–35.
9. Sutiningsih D, Mustofa, Satoto TBT, Mar-tono E (2017). Inhibitory effects of bruceine A biolarvicide on growth and development of Aedes aegypti larvae. J En-tomol, 14:104–11.
10. Sutiningsih D, Mustofa, Satoto TBT, Mar-tono E (2017). Neurotoxic mechanism of bruceine A biolarvicide against Aedes ae-gypti Linnaeus larvae. Res J Med Plants, 11:77–85.
11. WHO (2005). Guidelines for laboratory and field testing of mosquito larvicides. World Health Organization. Geneva.
12. Sharma A, Kumar S, Tripathi P (2015). Im-pact of Achyranthes aspera leaf and stem extracts on the survival, morphology and behaviour of an Indian strain of dengue vector, Aedes aegypti L (Diptera: Culicidae). JMR, 5 (7):1–9.
13. Dahlan M (2014). Statistic for medicine and health. 6 th ed. Indonesian Epidemiology. Jakarta.
14. Farnesi LC, Brito JM, Linss JG, Pelajo-Machado M, Valle D, Rezende GL (2012). Physiological and morphological aspect of Aedes aegypti developing larvae: Effects of the chitin synthesis inhibitor novaluron. PloS ONE, 7 (1): e30363.
15. Pesticide Commission (1995). Standard method of pesticide effication test. De-partment of Agriculture. Jakarta.
16. Matsumura F (1985). Toxicology of insecticides. 2nd ed. Plenum Press. New York.
17. Wahyuni D (2015). New bioinsecticide gran-ules toxin from extract of papaya (Carica papaya) seed and leaf modified against Aedes aegypti larvae. Procedia Environ Sci, 23:323–8.
18. Sanjaya Y, Safaria T (2006). Toxicity poison from spider Nephila sp. to Aedes aegypti L. larvae. J Biodiversitas, 7:191–4.
19. Choochote W, Tuetun B, Kanjanapothi D et al (2004). Potential of crude seed extract of celery, Apium graveolens L., against the mosquito Aedes aegypti (L.) (Diptera: Cu-licidae). J Vector Ecol, 29 (2):340–6.
20. Dharmagadda VS, Naik SN, Mittal PK, Vasudevan P (2005). Larvicidal activity of Tagetes patula essential oil against three mosquito species. Bioresour Technol, 96 (11):1235–40.
21. Chaithong U, Choochote W, Kamsuk K et al (2006). Larvicidal effect of pepper plants on Aedes aegypti (L.) (Diptera: Cu-licidae). J Vector Ecol, 31 (1):138–44.
22. Kumar S, Warikoo R, Wahab N (2010). Lar-vicidal potential of ethanolic extracts of dried fruits of three species of pepper-corns against different instars of an Indi-an strain of dengue fever mosquito, Aedes aegypti L. (Diptera: Culicidae). Parasitol Res, 107 (4):901–7.
23. Tjokropranoto R, Evacuasiany E, Saputro N (2010). Efectiveness of Beluntas infusion (Plucea indica L.) as larvicide againts Aedes sp. Med Planta, 1 (2):75–80.
24. Syahputra E (2008). Bioactivity of Brucea ja-vanica fruit as nabatical insecticide for agri-cultural insect pest. Bul Littro, 19 (1):56–67.
25. Lina E, Dadang, Manuwoto S, Syahbirin G, Prijono D (2013). Synergistic action of mixed extract Brucea javanica (Simarou-baceae), Piper aduncum (Piperaceae), and Tephrosia vogelli (Leguminosae) against cabbage head caterpillar, Crocidolomia pavo-nana. J Biopestic, 6 (1):77–83.
26. Lu F, Kacew S (2002). Lu’s basic toxicology : Fundamentals, targets organ and risk assesment. 4th ed. Taylor & Francis. New York.
27. Wulandari S, Arnetis, Rahayu S (2012). Po-tential of sap Papaya fruit (Carica papaya L) against mortality of Aedes albopictus mos-quitoes larvae. Biogenesis, 9 (1):69–75.
28. Kringer R (2010). Handbook of pesticide toxicolo-gy. Academic Press. California.
29. Warikoo R, Kumar S (2013). Impact of Argemone mexicana extracts on the cidal, morphological, and behavioural response of dengue vector, Aedes aegypti L. (Diptera: Culicidae). Parasitol Res, 112 (10):3477–84.
30. Insun D, Choochote W, Jitpakdi A, Chaithong U, Tippawangkosol P, Pita-sawat B (1999). Possible site of action of Kaempferia galanga in killing Culex quinquefas-ciatus larvae. Southeast Asian J Trop Med Pub-lic Health, 30 (1):195–9.
|Issue||Vol 48 No 4 (2019)|
|Larvicide Brusatol Brucea javanica (L) Merr Culex quinquefasciatus|
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