Larvicidal Activity of Brusatol Isolated from Brucea javanica (L) Merr on Culex quinquefasciatus

  • Dwi SUTININGSIH Mail Department of Epidemiology and Tropical Diseases, Faculty of Public Health, Diponegoro University, Semarang, Indonesia
  • Nurjazuli NURJAZULI Department of Environmental Health, Faculty of Public Health, Diponegoro University, Semarang, Indonesia
  • Djoko NUGROHO Department of Biostatistics, Faculty of Public Health, Diponegoro University, Semarang, Indonesia
  • Tri Baskoro Tunggul SATOTO Department of Parasitology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia
Keywords:
Larvicide, Brusatol, Brucea javanica (L) Merr, Culex quinquefasciatus

Abstract

Background: Vector control is still emphasized on the using of chemical insecticide which can cause death of non-target organisms, pollution and vector resistance. Therefore, natural insecticides/larvicides are an alternative to using chemical insecticides to control the mosquito vector.

Methods: Brusatol was isolated from the seeds of Makassar Fruit (Brucea javanica L. Merr). Culex quinquefasciatus larvae were divided into 3 groups, i.e. 6 testing groups and one negative and positive control group. In the negative control group, the larvae were treated with 100 ml aquadest and positive control was treated with temephos 1 ppm. After 24 hours, dead larvae were calculated and the percentage of death was determined. The dead larvae were then examined for morphological changes using a light microscope.

Results: The higher of the concentration level of brusatol, the higher number of the death of Cx. quinquefasciatus larvae (P<0.05). The value of brusatol Lethal Concentration 50 to larvae Cx. quinquefasciatus was 0.010 ± 0.122 and value of brusatol Lethal Concentration 90 to larvae Cx. quinquefasciatus was 0.654 ± 0.081 ppm. The higher the concentration of brusatol, the morphological damage of Cx. quinquefasciatus larvae was getting worse and widespread to cause damage to the digestive tract and cuticle.

Conclusion: Brusatol isolated from the seed of B. javanica (L) Merr have larvicidal activity to the Cx. quinquefasciatus which is potential to be natural larvicide.

 

 

References

1. Jansen CC, Beebe N (2010). The dengue vector of Aedes aegypti : What comes next. Microbes Infect, 12 (4):272–9.
2. Isman MB (2006). Botanical insecticides, de-terrents, and repellents in modern agricul-ture and an increasingly regulated world. Annu Rev Entomol, 51:45–66.
3. Isman MB (2008). Botanical insecticides: for richer, for poorer. Pest Manag Sci, 64 (1):8–11.
4. Takeya K, Kim I, Hitotsuyanagi Y, Hasuda T (2006). Antitumour quassinoid from Brucea javanica and SAR of Brusatol. Planta Med, 72:20–4.
5. Govindachari T, Krishna KGN, Gopala-krishnan G, Suresh G, Wesley S, Seelatha T (2001). Insect antifeedant and growth regulating activities of quassinoids from Samadera indica. Fitoterapia, 72:568–71.
6. Chen YY, Pan Q, Li D, Liu J, Wen Y, Huang Y, Lu F (2011). New pregnane glycosides from Brucea javanica and their antifeedant activity. Chem Biodivers, 31:460–6.
7. Zhang L, Feng X, Ma D, Yang J, Jiang H, Zhang Y, He W (2013). Brusatol isolated from Brucea javanica (L) Merr induces apoptotic death of insect cell lines. Pestic Biochem Physiol, 107 (1):18–24.
8. Sutiningsih D, Nurjazuli (2017). Effect of brusatol biolarvicide administration on behavioral response of Aedes aegypti and its toxicity on Vero cells. J Biol Sci, 17:127–35.
9. Sutiningsih D, Mustofa, Satoto TBT, Mar-tono E (2017). Inhibitory effects of bruceine A biolarvicide on growth and development of Aedes aegypti larvae. J En-tomol, 14:104–11.
10. Sutiningsih D, Mustofa, Satoto TBT, Mar-tono E (2017). Neurotoxic mechanism of bruceine A biolarvicide against Aedes ae-gypti Linnaeus larvae. Res J Med Plants, 11:77–85.
11. WHO (2005). Guidelines for laboratory and field testing of mosquito larvicides. World Health Organization. Geneva.
12. Sharma A, Kumar S, Tripathi P (2015). Im-pact of Achyranthes aspera leaf and stem extracts on the survival, morphology and behaviour of an Indian strain of dengue vector, Aedes aegypti L (Diptera: Culicidae). JMR, 5 (7):1–9.
13. Dahlan M (2014). Statistic for medicine and health. 6 th ed. Indonesian Epidemiology. Jakarta.
14. Farnesi LC, Brito JM, Linss JG, Pelajo-Machado M, Valle D, Rezende GL (2012). Physiological and morphological aspect of Aedes aegypti developing larvae: Effects of the chitin synthesis inhibitor novaluron. PloS ONE, 7 (1): e30363.
15. Pesticide Commission (1995). Standard method of pesticide effication test. De-partment of Agriculture. Jakarta.
16. Matsumura F (1985). Toxicology of insecticides. 2nd ed. Plenum Press. New York.
17. Wahyuni D (2015). New bioinsecticide gran-ules toxin from extract of papaya (Carica papaya) seed and leaf modified against Aedes aegypti larvae. Procedia Environ Sci, 23:323–8.
18. Sanjaya Y, Safaria T (2006). Toxicity poison from spider Nephila sp. to Aedes aegypti L. larvae. J Biodiversitas, 7:191–4.
19. Choochote W, Tuetun B, Kanjanapothi D et al (2004). Potential of crude seed extract of celery, Apium graveolens L., against the mosquito Aedes aegypti (L.) (Diptera: Cu-licidae). J Vector Ecol, 29 (2):340–6.
20. Dharmagadda VS, Naik SN, Mittal PK, Vasudevan P (2005). Larvicidal activity of Tagetes patula essential oil against three mosquito species. Bioresour Technol, 96 (11):1235–40.
21. Chaithong U, Choochote W, Kamsuk K et al (2006). Larvicidal effect of pepper plants on Aedes aegypti (L.) (Diptera: Cu-licidae). J Vector Ecol, 31 (1):138–44.
22. Kumar S, Warikoo R, Wahab N (2010). Lar-vicidal potential of ethanolic extracts of dried fruits of three species of pepper-corns against different instars of an Indi-an strain of dengue fever mosquito, Aedes aegypti L. (Diptera: Culicidae). Parasitol Res, 107 (4):901–7.
23. Tjokropranoto R, Evacuasiany E, Saputro N (2010). Efectiveness of Beluntas infusion (Plucea indica L.) as larvicide againts Aedes sp. Med Planta, 1 (2):75–80.
24. Syahputra E (2008). Bioactivity of Brucea ja-vanica fruit as nabatical insecticide for agri-cultural insect pest. Bul Littro, 19 (1):56–67.
25. Lina E, Dadang, Manuwoto S, Syahbirin G, Prijono D (2013). Synergistic action of mixed extract Brucea javanica (Simarou-baceae), Piper aduncum (Piperaceae), and Tephrosia vogelli (Leguminosae) against cabbage head caterpillar, Crocidolomia pavo-nana. J Biopestic, 6 (1):77–83.
26. Lu F, Kacew S (2002). Lu’s basic toxicology : Fundamentals, targets organ and risk assesment. 4th ed. Taylor & Francis. New York.
27. Wulandari S, Arnetis, Rahayu S (2012). Po-tential of sap Papaya fruit (Carica papaya L) against mortality of Aedes albopictus mos-quitoes larvae. Biogenesis, 9 (1):69–75.
28. Kringer R (2010). Handbook of pesticide toxicolo-gy. Academic Press. California.
29. Warikoo R, Kumar S (2013). Impact of Argemone mexicana extracts on the cidal, morphological, and behavioural response of dengue vector, Aedes aegypti L. (Diptera: Culicidae). Parasitol Res, 112 (10):3477–84.
30. Insun D, Choochote W, Jitpakdi A, Chaithong U, Tippawangkosol P, Pita-sawat B (1999). Possible site of action of Kaempferia galanga in killing Culex quinquefas-ciatus larvae. Southeast Asian J Trop Med Pub-lic Health, 30 (1):195–9.
Published
2019-04-16
How to Cite
1.
SUTININGSIH D, NURJAZULI N, NUGROHO D, SATOTO TBT. Larvicidal Activity of Brusatol Isolated from Brucea javanica (L) Merr on Culex quinquefasciatus. Iran J Public Health. 48(4):688-696.
Section
Original Article(s)