Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 4 2019 04 16 688 696 Dwi SUTININGSIH Department of Epidemiology and Tropical Diseases, Faculty of Public Health, Diponegoro University, Semarang, Indonesia Nurjazuli NURJAZULI Department of Environmental Health, Faculty of Public Health, Diponegoro University, Semarang, Indonesia Djoko NUGROHO Department of Biostatistics, Faculty of Public Health, Diponegoro University, Semarang, Indonesia Tri Baskoro Tunggul SATOTO Department of Parasitology, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia 2019 04 16 Background: Vector control is still emphasized on the using of chemical insecticide which can cause death of non-target organisms, pollution and vector resistance. Therefore, natural insecticides/larvicides are an alternative to using chemical insecticides to control the mosquito vector. Methods: Brusatol was isolated from the seeds of Makassar Fruit (Brucea javanica L. Merr). Culex quinquefasciatus larvae were divided into 3 groups, i.e. 6 testing groups and one negative and positive control group. In the negative control group, the larvae were treated with 100 ml aquadest and positive control was treated with temephos 1 ppm. After 24 hours, dead larvae were calculated and the percentage of death was determined. The dead larvae were then examined for morphological changes using a light microscope. Results: The higher of the concentration level of brusatol, the higher number of the death of Cx. quinquefasciatus larvae (P<0.05). The value of brusatol Lethal Concentration 50 to larvae Cx. quinquefasciatus was 0.010 ± 0.122 and value of brusatol Lethal Concentration 90 to larvae Cx. quinquefasciatus was 0.654 ± 0.081 ppm. The higher the concentration of brusatol, the morphological damage of Cx. quinquefasciatus larvae was getting worse and widespread to cause damage to the digestive tract and cuticle. Conclusion: Brusatol isolated from the seed of B. javanica (L) Merr have larvicidal activity to the Cx. quinquefasciatus which is potential to be natural larvicide.     https://ijph.tums.ac.ir/index.php/ijph/article/view/16860 https://ijph.tums.ac.ir/index.php/ijph/article/download/16860/6356