COMPARISON OF EXCRETORY SECRETORY ANTIGENS OF ASCARIS LUMBRICOLDES AND TOXOCARA CATI 2ND. STAGE LARVAE WITH BIOCHEMICAL METHODES

Abstract

Purification of parasitic antigens is a major activity in immunoparasitology because of its application in immunodiagnosis, vaccination analysis of immunopathology, preparation of monocolomal antibody and finding out the cross reactive antigens versus specific antigens of a parasite. In this survey F2 ultrafiltration excretory secretory antigens of Ascaris lumbricoides and Toxocara cati were separated by gel chromatography on sephacryl S-200 into several antigenic and non antigenic fractions. The E/S antigens of this nematode were analyzed by using soudium dodecyl sulfate polyacryl amide gel electrophoresis (SDS PAGE analysis of Ascaris lumbricoides and Toxocara cati revealed a minimum of 12 KD to 130 KD distinct with brilliant coomassie blue staining bands respectively.