Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 24 3-4 1995 12 15 55 62 F. Maleky J. Massoud 2015 10 03 Purification of parasitic antigens is a major activity in immunoparasitology because of its application in immunodiagnosis, vaccination analysis of immunopathology, preparation of monocolomal antibody and finding out the cross reactive antigens versus specific antigens of a parasite. In this survey F2 ultrafiltration excretory secretory antigens of Ascaris lumbricoides and Toxocara cati were separated by gel chromatography on sephacryl S-200 into several antigenic and non antigenic fractions. The E/S antigens of this nematode were analyzed by using soudium dodecyl sulfate polyacryl amide gel electrophoresis (SDS PAGE analysis of Ascaris lumbricoides and Toxocara cati revealed a minimum of 12 KD to 130 KD distinct with brilliant coomassie blue staining bands respectively. https://ijph.tums.ac.ir/index.php/ijph/article/view/1549 https://ijph.tums.ac.ir/index.php/ijph/article/download/1549/1530