Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells

  • Yizhuo LU Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China
  • Lianghui LI Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China
  • Guoyang WU Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China
  • Huiqin ZHUO Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen, P.R. China
  • Guoyan LIU Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China
  • Jianchun CAI 1. Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen, P.R. China 2. Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China
Keywords: Gastric cancer; LY294002; MK-2206; Phosphorylation; PI3K/Akt signaling pathway; PRAS40-Thr246

Abstract

Abstract

Background: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells.

Methods: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly.  

Results: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway.

Conclusion: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors.

 

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Published
2019-12-02
How to Cite
1.
LU Y, LI L, WU G, ZHUO H, LIU G, CAI J. Effect of PI3K/Akt Signaling Pathway on PRAS40Thr246 Phosphorylation in Gastric Cancer Cells. Iran J Public Health. 48(12):2196-2204.
Section
Original Article(s)