Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 12 2019 12 02 2196 2204 Yizhuo LU Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China Lianghui LI Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China Guoyang WU Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China Huiqin ZHUO Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen, P.R. China Guoyan LIU Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China Jianchun CAI Institute of Gastrointestinal Oncology, School of Medicine, Xiamen University, Xiamen, P.R. China AND Department of Gastrointestinal Surgery, Zhongshan Hospital, Xiamen University, Xiamen, P.R. China 2019 12 02 Background: We aimed to investigate the effect of PI3K/Akt signaling pathway on PRAS40Thr246 phosphorylation in gastric cancer cells. Methods: The study was conducted from April 2017 to January 2018 in Zhongshan Hospital, Xiamen University, Xiamen, China. Gastric cancer cells were divided into three groups: gastric cancer cell group, LY294002 group and MK-2206 group. Specific tests were conducted accordingly.   Results: Inhibition of PI3K/Akt signaling pathway activation and PRAS40Thr246 phosphorylation could inhibit proliferation and invasion and promote apoptosis of gastric cancer cells, and PRAS40Thr246 phosphorylation could activate PI3K/Akt signaling pathway. Conclusion: The levels of PI3K/Akt signaling pathway related proteins and p-PRAS40Thr246 were significantly increased in gastric cancer cells. p-PRAS40-Thr246 was able to reflect the activation of the PI3K/Akt signaling pathway, reflecting the sensitivity of the PI3K/AKT signaling pathway to inhibitors. https://ijph.tums.ac.ir/index.php/ijph/article/view/19068 https://ijph.tums.ac.ir/index.php/ijph/article/download/19068/6600