Evaluation of the Effects of Local Application of Thyme Honey in Open Cutaneous Wound Healing
-
Nasrin TAKZAREE
,
Gholamreza HASSANZADEH
,
Mohammad Reza ROUINI
,
Azadeh MANAYI
,
Abbas HADJIAKHONDI
,
Masoumeh MAJIDI ZOLBIN
Abstract
Background: Clinicians have been searching for ways to obtain "super normal" wound healing. Honey is a traditional remedy for the treatment of infected wounds. We aimed to evaluate the wound contraction and antibacterial properties of locally produced Thyme honey on managing full-thickness wounds in vivo.
Methods: This experimental study was conducted in 2015, in Department of Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran on 54 adult male Wistar rats weighing 200-250 gr, and ages of 3-4 months. A square 1.5*1.5 wound was made on the back of the neck. The rats were divided into control and two experimental groups. Additionally, the control and experimental groups were separated into three subgroups corresponding to 4, 7, and 14 d of study. The control group did not receive any treatment. For histological studies, samples were taken from the wound and adjacent skin. This tissue was examined using histological staining (H&E). Wound surface and wound healing were evaluated. Data were analyzed by using one-way ANOVA with post hoc Tukey test and (P<0.05) was significant.
Results: The macroscopic and microscopic evaluations showed that the percentage of wound healing on different days in the control and experimental groups were significant (P< 0.05).
Conclusion: Using honey twice a day on open wounds will accelerate the healing process.
Goldberg SR, Diegelmann RF (2010). Wound healing primer. Surg Clin North Am, 90:1133-1146.
Jurjus A, Atiyeh BS, Abdallah IM, et al (2007). Pharmacological modulation of wound healing in experimental burns. Burns, 33:892-907.
Pazyar NY, Yaghoobi R, Rafiee E, Mehrabian A, Feily A (2014). Skin wound healing and phytomedicine: a review. Skin Pharmacol Physiol, 27:303-10.
Takzare N, Hosseini MJ, Hasanzadeh G, Mortazavi H, Takzare A, Habibi P (2009). Influence of Aloe Vera gel on dermal wound healing process in rat. Toxicol Mech Methods, 19:73-77.
Brölmann F, Ubbink D, Nelson E, Munte K, Van Der Horst C, Vermeulen H (2012). Evidence‐based decisions for local and systemic wound care. Br J Surg, 99:1172-1183.
Bowler PG (2002). Wound pathophysiology, infection and therapeutic options. Ann Med, 34:419-427.
Linden L, Emmans P 3rd, Safranek S (2014). Topical preparations for wound healing. Am Fam Physician, 89:978-9.
Dorai AA (2012). Wound care with traditional, complementary and alternative medicine. Indian J Plast Surg, 45: 418–424.
Dunphy JE, Udupa K (1955). Chemical and histochemical sequences in the normal healing of wounds. N Engl J Med, 253:847-851.
Sivamani RK, Ma BR, Wehrli LN, Maverakis E (2012). Phytochemicals and Naturally Derived Substances for Wound Healing. Adv Wound Care (New Rochelle), 1:213-217.
Efem S (1988). Clinical observations on the wound healing properties of honey. Br J Surg, 75:679-681.
Jull AB, Rodgers A, Walker N (2008). Honey as a topical treatment for wounds. Cochrane Database Syst Rev, (4):CD005083.
Bose B (1982). Honey or sugar in treatment of infected wounds? Lancet, 1:963.
Molan PC (1992). The antibacterial activity of honey: 1. The nature of the antibacterial activity. Bee World, 73:5-28.
Lien Y-H, Tseng MM, Stern R (1992). Glucose and glucose analogs modulate collagen metabolism. Exp Mol Pathol, 57:215-21.
Basson NJ, Grobler SR (2008). Antimicrobial activity of two South African honeys produced from indigenous Leucospermum cordifolium and Erica species on selected micro-organisms. BMC Complement Altern Med, 8:41.
Pruitt KM, Tenovuo JO (1985). The lactoperoxidase system. Chemistry and biological significance. 1st Ed. https://www.amazon.com/Lactoperoxidase-System-Biological-Significance-Immunology/dp/0824772989
Armon P (1980). The use of honey in the treatment of infected wounds. Trop Doct, 10:91.
Cooke J, Dryden M, Patton T, Brennan J, Barrett J (2015). The antimicrobial activity of prototype modified honeys that generate reactive oxygen species (ROS) hydrogen peroxide. BMC Res Notes, 8:20.
Khan F, Hill J, Kaehler S, Allsopp M, Vuuren S (2014). Antimicrobial properties and isotope investigations of South African honey. J Appl Microbiol, 117:366-79.
Bergman A, Yanai J, Weiss J, Bell D, David MP (1983). Acceleration of wound healing by topical application of honey: an animal model. Am J Surg, 145:374-376.
Burlando B, Cornara L (2013). Honey in dermatology and skin care: a review. J Cosmet Dermatol, 12:306-313.
Abuharfeil N, Al-Oran R, Abo-Shehada M (1999). The effect of bee honey on the proliferative activity of human B-and T-lymphocytes and the activity of phagocytes. Food Agric Immunol, 11:169-177.
Raynaud A, Ghezali L, Gloaguen V, Liagre B, Quero F, Petit J-M (2013). Honey-induced macrophage stimulation: AP-1 and NF-κB activation and cytokine production are unrelated to LPS content of honey. Int Immunopharmacol, 17:874-9.
Yaghoobi R, Kazerouni A (2013). Evidence for clinical use of honey in wound healing as an anti-bacterial, anti-inflammatory anti-oxidant and anti-viral agent: A review. Jundishapur J Nat Pharm Prod, 8:100-104.
Sell SA, Wolfe PS, Spence AJ, Rodriguez IA, McCool JM, et al (2012). A preliminary study on the potential of manuka honey and platelet-rich plasma in wound healing. Int J Biomater, 2012: 313781.
Ranzato E, Martinotti S, Burlando B (2012). Epithelial mesenchymal transition traits in honey‐driven keratinocyte wound healing: Comparison among different honeys. Wound Repair Regen, 20:778-785.
Barui A, Mandal N, Majumder S et al (2013). Assessment of molecular events during in vitro re-epithelialization under honey-alginate matrix ambience. Mater Sci Eng C Mater Biol Appl, 33:3418-25.
Cooper, R, Molan, P , Krishnamoorthy, L, Harding, K (2001). Manuka honey used to heal a recalcitrant surgical wound. Eur J Clin Microbiol Infect Dis, 20:758-9.
| Files | ||
| Issue | Vol 46 No 4 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| Open cutaneous wound Honey Wound healing Rat | ||
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