Molecular and Morphometrical Characterization of Fasciola Species Isolated from Domestic Ruminants in Ardabil Province, Northwestern Iran
Background: We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran.
Methods: Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp. based on morphological and metric assessment of external features of fresh adults, morphological and metric assessment of internal anatomy of stained mounted worms. Statistical analysis was conducted using the Student’s t-test implemented in SPSS 15.0 (SPSS, Chicago, Illinois). Then the morphometric criteria of Fasciola samples were compared with PCR-RFLP data. The results of PCR-RFLP were confirmed by COI gene sequence.
Results: The differences between the body length, area of the body, peripheral of the body, succer area, cone length, cone width, in two species were significant (
Mas‐Coma S, Valero MA, Bargues MD (2009). Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology evolutionary genetics, molecular epidemiology and control. Adv Parasitol, 69: 41-146.
Mas-Coma S, Bargues M, Valero M (2005). Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol, 35(11-12):1255-78.
World Health Organization. The "neglected" neglected worms. Action against Worms. 2007; 10:1-8.
Ashrafi K (2015). The Status of Human and Animal Fascioliasis in Iran: A Narrative Review Article. Iran J Parasitol, 10(3):306-28.
Haroun E, Hillyer GV (1986). Resistance to fascioliasis--a review. Vet Parasitol, 20(1-3):63-93.
Valero MA, Bargues MD, Khoubbane M, Artigas P, Quesada C, Berinde L, Ubeira FM, Mezo M, Hernandez JL, Agramunt VH (2016). Higher physiopathogenicity by Fasciola gigantica than by the genetically close F. hepatica: experimental long-term follow-up of biochemical markers. Trans R Soc Trop Med Hyg, 110(1):55-66.
Amor N, Halajian A, Farjallah S, Merella P, Said K, Slimane BB (2011). Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences. Exp Parasitol, 128(3):196-204.
Ashrafi K, Valero M, Panova M, Periago M, Massoud J, Mas-Coma S (2006). Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int, 55(4):249-60.
El-Rahimy HH, Mahgoub AM, El-Gebaly NSM, Mousa WM, Antably AS (2012). Molecular, biochemical, and morphometric characterization of Fasciola species potentially causing zoonotic disease in Egypt. Parasitol Res, 111(3):1103-11.
Asadian S, Mohebali M, Mahmoudi M, Kia EB, Heidari Z, Asgari M, Aryaeipour M, Moradi S, Rokni MB (2013). Seroprevalence of human fascioliasis in Meshkin-Shahr district, Ardabil Province, northwestern Iran in 2012. Iran J Parasitol, 8(4):516-21.
Daryani A, Alaei R, Arab R, Sharif M, Dehghan M, Ziaei H (2006). Prevalence of liver fluke infections in slaughtered animals in Ardabil province, Northwestern Iran. J Anim Vet Adv, 5(5):408-411.
Aryaeipour M, Rouhani S, Bandehpour M, Mirahmadi H, Kazemi B, Rokni MB (2014). Genotyping and phylogenetic analysis of Fasciola spp. isolated from sheep and cattle using PCR-RFLP in Ardabil province, northwestern Iran. Iran J Public Health, 43(10):1364-71.
Itagaki T, Kikawa M, Sakaguchi K, Shimo J, Terasaki K, Shibahara T, Fukuda K (2005). Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology, 131(05):679-85.
Farjallah S, Sanna D, Amor N, Mehel BB, Piras MC, Merella P, Casu M, Curini-Galletti M, Said K, Garippa G (2009). Genetic characterization of Fasciola hepatica from Tunisia and Algeria based on mitochondrial and nuclear DNA sequences. Parasitol Res, 105(6):1617-21.
Teofanova D, Yoveva A, Radoslavov G, Hristov P. Issues associated with genetic diversity studies of the liver fluke, Fasciola heptica (Platyhelminthes, Digenea, Fasciolidae). INTECH Open Access Publisher; 2012.
Sahba G, Arfaa F, Farahmandian I, and Jalali H (1972). Animal fascioliasis in Khuzestan, southwestern Iran. J Parasitol, 58(4):712-6.
Kimura S, Shimizu A, and Kawano J (1984). Morphological observation on liver fluke detected from naturally infected carabaos in the Philippines. Sci Rept Fac Agr Kobe Univ, 16: 353-7.
Varma A (1953). On Fasciola indica n. sp. with some observations on F. hepatica and F. gigantica. J Helminthol, 27(3-4):185-98.
Periago M, Valero M, Panova M, Mas-Coma S (2006). Phenotypic comparison of allopatric populations of Fasciola hepatica and Fasciola gigantica from European and African bovines using a computer image analysis system (CIAS). Parasitol Res. 99(4):368-78.
Periago M, Valero M, El Sayed M, Ashrafi K, El Wakeel A, Mohamed M, Desquesnes M, Curtale F, Mas-Coma S (2008). First phenotypic description of Fasciola hepatica/Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt. Infect Genet Evol, 8(1):51-8.
Moghaddam A, Massoud J, Mahmoodi M, Mahvi A, Periago M, Artigas P, Fuentes M, Bargues M, Mas-Coma S (2004). Human and animal fascioliasis in Mazandaran province, northern Iran. Parasitol Res, 94(1):61-9.
Chaudhry U, van Paridon B, Shabbir M, Shafee M, Ashraf K, Yaqub T, Gilleard J (2015). Molecular evidence shows that the liver fluke Fasciola gigantica is the predominant Fasciola species in ruminants from Pakistan. J Helminthol, 90(2):206-13.
Le TH, De NV, Agatsuma T, Thi Nguyen TG, Nguyen QD, McManus DP, Blair D (2008). Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam. Int J Parasitol, 38(6):725-30.
Amer S, ElKhatam A, Zidan S, Feng Y, Xiao L (2016). Identity of Fasciola spp. In sheep in Egypt. Parasit Vectors, 9(1):623.
Yakhchali M, Malekzadeh-Viayeh R, Imani-Baran A, Mardani K (2015). Morphological and molecular discrimination of Fasciola species isolated from domestic ruminants of Urmia city, Iran. Iran J Parasitol, 10(1):46-55.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.