Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran
Background: We evaluated the genetic diversity of samples identiﬁed morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.
Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.
Results: In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study.
Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran.
Mas‐Coma S, Valero MA, Bargues MD (2009). Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Adv Parasitol, 69:41-146.
Mas-Coma S, Bargues M, Valero M (2005). Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol, 35(11):1255-78.
WHO, 2012. Accelerating Work to Overcome the Global Impact of Neglected Tropical Diseases; A Roadmap for Implementation – Executive Summary.
MAS CS, Bargues M (1997). Human liver flukes: a review. Res Rev Parasitol, 57(3-4):145-218.
Ashrafi K, Valero M, Panova M, Periago M, Massoud J, Mas-Coma S (2006). Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int. 55(4):249-60.
Teofanova D, Yoveva A, Radoslavov G, Hristov P. Issues Associated with Genetic Diversity Studies of the Liver Fluke, Fasciola heptica (Platyhelminthes, Digenea, Fasciolidae). INTECH Open Access Publisher; 2012.
Rokni M, Mirhendi H, Behnia M, Jalalizand N (2010). Molecular characterization of Fasciola hepatica isolates by RAPD-PCR and ribosomal ITS1 sequencing. Iran Red Crescent Med J, 2010(1):27-32.
Rokni MB, Mirhendi H, Mizani A, Mohebali M, Sharbatkhori M, Kia EB, Abdoli H, Izadi S (2010). Identification and differentiation of Fasciola hepatica and Fasciola gigantica using a simple PCR-restriction enzyme method. Exp Parasitol, 124(2):209-13.
Aryaeipour M, Rouhani S, Bandehpour M, Mirahmadi H, Kazemi B, Rokni MB (2014). Genotyping and Phylogenetic Analysis of Fasciola Spp. Isolated from Sheep and Cattle Using PCR-RFLP in Ardabil Province, Northwestern Iran. Iran J Public Health, 43(10):1364.
Shoriki T, Ichikawa-Seki M, Suganuma K, Naito I, Hayashi K, Nakao M, Aita J, Mohanta UK, Inoue N, Murakami K (2016). Novel methods for the molecular discrimination of Fasciola spp. on the basis of nuclear protein-coding genes. Parasitol Int, 65(3):180-3.
Ashrafi K (2015). The Status of Human and Animal Fascioliasis in Iran: A Narrative Review Article. Iran J Parasitol, 10(3):306.
Rokni M (2008). The present status of human helminthic diseases in Iran. Ann Trop Med Parasitol, 102(4):283-95.
Dalimi A, and Jabarvand M (2005). Fasciola hepatica in the human eye. Trans R Soc Trop Med Hyg, 99(10):798-800.
Hatami H, Asmar M, Masoud J, Mansouri F, Namdaritabar H, Ramazankhani A (2012). The first epidemic and new-emerging human fascioliasis in Kermanshah (western Iran) and a ten-year follow up, 1998-2008. Int J Prev Med, 3(4). 266-272.
Rokni MB, Lotfy WM, Ashrafi K, and Murrell KD. Neglected Tropical Diseases-Middle East and North Africa. Springer; 2014:59-90.
Parviz M, Rezaii M, Yahyaei M, Mozafari SH (2012). Survey of the rate of parasitic infections (Fasciola, Dicroceolium, hydatid cyst) in abbatoires of Kermanshah in 2009-2010. Iran J Vet Lab Res, 1: 4(1): 109.
Malone J, Gommes R, Hansen J, Yilma J, Slingenberg J, Snijders F, Nachtergaele F, Ataman E (1998). A geographic information system on the potential distribution and abundance of Fasciola hepatica F. gigantica in east Africa based on Food and Agriculture Organization databases. Vet Parasitol, 78(2):87-101.
Ashrafi K, Massoud J, Holakouei K, Mahmoodi M, Joafshani M, Valero M, Fuentes M, Khoubbane M, Artigas P, Bargues M (2004). Evidence suggesting that Fasciola gigantica might be the most prevalent causal agent of fascioliasis in northern Iran. Iran J Public Health, 33(4):31-7.
Amor N, Halajian A, Farjallah S, Merella P, Said K, Slimane BB (2011). Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences. Exp Parasitol, 128(3):196-204.
Wannasan A, Khositharattanakool P, Chaiwong P, Piangjai S, Uparanukraw P, Morakote N (2014). Identification of Fasciola species based on mitochondrial and nuclear DNA reveals the co-existence of intermediate Fasciola and Fasciola gigantica in Thailand. Exp Parasitol, 146(64-70.
Le TH, Van De N, Agatsuma T, Nguyen TGT, Nguyen QD, McManus DP, Blair D (2008). Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam. Int J Parasitol, 38(6):725-30.
Chaudhry U, van Paridon B, Shabbir M, Shafee M, Ashraf K, Yaqub T, and Gilleard J (2016). Molecular evidence shows that the liver fluke Fasciola gigantica is the predominant Fasciola species in ruminants from Pakistan. J Helminthol, 90(2):206-13.
Sharifiyazdi H, Moazeni M, Rabbani F (2012). Molecular characterization of human Fasciola samples in Gilan Province, Northern Iran on the basis of DNA sequences of ribosomal and mitochondrial DNA genes. Comp Clini Path, 21(5):889-94.
Itagaki T, Kikawa M, Sakaguchi K, Shimo J, Terasaki K, Shibahara T, Fukuda K. (2005). Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology, 131(05):679-85.
Amer S, Dar Y, Ichikawa M, Fukuda Y, Tada C, Itagaki T, Nakai Y (2011). Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers. Parasitol Int, 60(1):5-12.
Shalaby I, Gherbawy Y, and Banaja A (2013). Molecular characterization of Fasciola species isolated from imported sheep in Taif region (Saudi Arabia). Trop Biomed, 30(1):15-26.
Dar Y, Amer S, Mercier A, Courtioux B, Dreyfuss G (2012). Molecular identification of Fasciola spp.(Digenea: Fasciolidae) in Egypt. Parasite, 19(2):177-82.
Ichikawa M, and Itagaki T (2010). Discrimination of the ITS1 types of Fasciola spp. based on a PCR–RFLP method. Parasitol Res, 106(3):757-61.
Bargues MD, Artigas P, Khoubbane M, Flores R, Glöer P, Rojas-García R, Ashrafi K, Falkner G, Mas-Coma S (2011). Lymnaea schirazensis, an overlooked snail distorting fascioliasis data: Genotype, phenotype, ecology, worldwide spread, susceptibility, applicability. PLoS One, 6(9):e24567.
|Issue||Vol 45 No 10 (2016)|
|Fasciola hepatica Fasciola gigantica Genotype PCR Iran|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|