Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran
-
Arezoo BOZORGOMID
,
Naser NAZARI
,
Hamzeh RAHIMI
,
Eshrat BEIGOM KIA
,
Homa HAJJARAN
,
Mehdi MOHEBALI
,
Mojgan ARYAEIPOUR
,
Peyman HEIDARIAN
,
Mohammad Saeid EZATI
,
Mohamad Bagher ROKNI
Abstract
Background: We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.
Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.
Results: In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study.
Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran.
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| Issue | Vol 45 No 10 (2016) | |
| Section | Original Article(s) | |
| Keywords | ||
| Fasciola hepatica Fasciola gigantica Genotype PCR Iran | ||
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