Collagen Extracted from Persian Gulf Squid Exhibits Anti-Cytotoxic Properties on Apple Pectic Treated Cells: Assessment in an In Vitro Bioassay Model
,
Abstract
Background: Collagen-based three-dimensional (3D) in vitro systems have been introduced to study the physiological states of cells. As a biomolecule, collagen is usually extracted from terrestrial animals whilst aquatic animals like squid contain large amounts of collagen.
Methods: In order to make effective use of marine organisms, we selected Persian Gulf squid in 2015 to extract the required collagen. Then, a 3D culture system based on the extracted collagen was applied to investigate cellular mechanisms in a native microenvironment. The formed collagen gel was used to investigate the growth of MDA-MB-231 breast cancer cells as well as responses to pectic acid.
Results: The results revealed that the extracted collagen contained α, ß and γ components with high water holding capacity. This collagen formed a gel-like structure, which could promote the proliferation of MDA-MB-231 breast cancer cells. The MDA-MB-231 cells’ viability in presence of pectic acid, demonstrating the cells’ behavior in a 3D culture system.
Conclusion: It seems that the collagen extracted from squid skin has type I collagen properties. It might be used as a substrate in 3D cell culture systems.
Godoy P, Hewitt NJ, Albrecht U, Ander-sen ME, Ansari N, Bhattacharya S, Bode JG, Bolleyn J, et al. (2013). Re-cent advances in 2D and 3D in vitro systems using primary hepatocytes, al-ternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepa-totoxicity, cell signaling and ADME. Arch Toxicol, 87(8):1315-530.
Ma HL, Jiang Q, Han S, Wu Y, Cui Tomshine J, Wang D, Gan Y, Zou G, Liang XJ (2012). Multicellular tumor spheroids as an in vivo–like tumor model for three- dimensional imaging of chemotherapeutic and nano material cellular penetration. Mol Imag-ing, 11(6): 487–498.
Kunz-Schughart LA, Kreutz M, Knuechel R (1998). Multicellular spheroids: a three-dimensional in vitro culture system to study tumour biolo-gy. Int J Exp Pathol, 79(1):1-23.
Heppner GH, Miller BE (1989). Thera-peutic implication of tumor heteroge-neity. Semin Oncol, 16(2): 91-105.
Howes AL, Richardson RD, Finlay D, Vuori K (2014). 3-dimensional culture systems for anti-cancer compound profiling and high-throughput screen-ing reveal increases in EGFR inhibi-tor-mediated cytotoxicity compared to monolayer culture systems. PLoS One, 9(9): e108283.
Corn PG (2012). The tumor microenvi-ronment in prostate cancer: elucidat-ing molecular pathways for therapy development. Cancer Manag Res, 4: 183-93.
Breslin S, O'Driscoll L (2013). Three-dimensional cell culture: the missing link in drug discovery. Drug Discov To-day, 18(5-6):240-9.
Liu RH, Liu J, Chen B (2005). Apples prevent mammary tumors in rats. J Agric Food Chem, 53(6):2341-3.
Olano-Martin E, Rimbach GH, Gibson GR, Rastall RA (2003). Pectic and pectic oligosaccharides induce apop-tosis in in vitro human colonic adeno-carcinoma cells. Anticancer Res, 23(1A): 341–6.
Demotte N, Wieërs G, Van Der Smissen P, Moser M, Schmidt C, et al. (2010). A galectin3 legend corrects the im-paired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice. Cancer Res, 70(19): 7476–7488.
Morris V, Belshaw N, Waldron K, Max-well E (2013). The bioactivity of modified pectic fragments. Bioactive Carbohydrates and Dietary Fiber, 1(1): 21–37.
Li Y, Niu Y, Wu H, Sun Y, Li Q, Kong X, Liu L, Mei Q (2010). Modified ap-ple polysaccharides could induce apoptosis in colorectal cancer cells. J Food Sci, 75(8):H224-9.
Li Y, Liu L, Niu Y, Feng J, Sun Y, Kong X, Chen Y, Chen X, Gan H, Cao S, Mei Q (2012). Modified apple poly-saccharide prevents against tumor-igenesis in a mouse model of colitis-associated colon cancer: role of galec-tin-3 and apoptosis in cancer preven-tion. Eur J Nutr, 51(1):107–117.
Senaratnea LS, Park P, Kim S (2006). Isolation and characterization of col-lagen from brown backed toadfish (Lagocephalus gloveri) skin. Bioresour Technol, 97(2): 191-197.
AOAC. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. 1995.
Laemmli UK (1970). Cleavage of struc-tural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259):680-5.
Kolodziejska I, Sikorski ZE, Niecikow-ska C (1999). Parameters affecting the isolation of collagen from squid (Illex argentinus) skins. Food Chem, 66(2): 153–157.
Jenkins CL, Bretscher LE, Guzei IA, Raines RT (2003). Effect of 3-hydroxyproline residues on collagen stability. J Am Chem Soc, 125 (21): 6422-6427.
Lee C, Singla A, Lee Y (2001). Biomedi-cal applications of collagen. Int J Pharm, 221(1-2):1-22.
Cho SY, Klemke RL (2000). Extracellu-lar-regulated kinase activation and Cas/Crk coupling regulate cell migra-tion and suppress apoptosis during in-vasion of the extracellular matrix. J Cell Biol, 149(1): 223–236.
Tibbitt MW, Anseth KS (2009). Hydro-gels as extracellular matrix mimics for 3D cell culture. Biotechnol Bioeng, 103(4): 655-63.
Jongjareonrak A, Benjakul S, Visessan-guan W, Nagai T, Tanaka, M (2005). Isolation and characterization of acid and pepsin-solubilised collagens from the skin of Brown stripe red snapper (Lutjanus vitta). Food Chem, 93: 475-484.
Yata M, Yoshida C, Fujisawa S, Mizuta S, Yoshinaka R (2001). Identification and characterization of molecular spe-cies of collagen in fish skin. J Food Sci, 66(2): 247–251.
Szot CS, Buchanan CF, Freeman JW, Rylander MN (2011). 3D in vitro bio-engineered tumors based on collagen I hydrogels. Biomaterials, 32(31): 7905-12.
Kim JB (2005). Three-dimensional tissue culture models in cancer biology. Semin Cancer Biol, 15(5): 365–377.
Hutmacher DW, Horch RE, Loessner D, Rizzi S, Sieh S, Reichert JC, et al. (2009). Translating tissue engineering technology platforms into cancer re-search. J Cell Mol Med, 13(8A): 1417-27.
Delphi L, Sepehri H, Khorramizadeh MR, Mansoori F (2015). Pectic-Oligosaccharides from Apples Induce Apoptosis and Cell Cycle Arrest in MDA-MB-231 Cells, a Model of Hu-man Breast Cancer. Asian Pac J Cancer Prev, 16(13):5265-5271.
Koutsilieris M, Reyes-Moreno C, Choki I, Sourla A, Doillon C, Pavlidis N (1999). Chemotherapy cytotoxicity of human MCF-7 and MDA-MB 231 breast cancer cells is altered by osteo-blast-derived growth factors. Mol Med, 5(2): 86-97.
Krause S, Maffini M, Soto A, Sonnen-schein C (2010). The microenviron-ment determines the breast cancer cells' phenotype: organization of MCF7 cells in 3D cultures. BMC Can-cer, 10:263-270.
Fang JY, Tan SJ, Yang Z, Tayag C, Han B (2014). Tumor bioengineering using a transglutaminase crosslinked hydro-gel. PLos One, 9(8): e105616.
| Files | ||
| Issue | Vol 45 No 8 (2016) | |
| Section | Original Article(s) | |
| Keywords | ||
| Cell culture Collagen Pectic acid Squid 3D culture system | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
