Determination of HBV Genotypes among Hbs Ag Positive Blood Donors in Tehran, Iran Using PCR-RFLP

Abstract

Background: Hepatitis B virus (HBV) is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a comparison of complete genomic se­quences, HBV has been classified into eight genotypes A-H which show a geographical distribution. Some genotypes are associ­ated with different clinical outcomes. Identification of HBV genotypes is important to begin and follow up the treat­ment.
Methods: In this cross-sectional study, the serum samples of blood donors were collected from Tehran Blood Transfusion Cen­ters in period during "2005-2006". Sera of 55 blood donors who were positive for hepatitis B surface antigen were se­lected. DNA was extracted using commercial kit and the S gene sequence was amplified by nested-PCR. PCR products were then analyzed for restriction enzymes that would be genotype specific.
Results: Genotype D was found the only type in all HBV DNA positive serum samples, in Tehran.
Conclusion: Genotype D is dominant among Tehran's blood donors, which is consistent with Iran and the Middle East domi­nant genotype.