Immunohistochemistry Analysis of P53 and Ki-67 Proteins in Oral Lichen Planus and Normal Oral Mucosa

Abstract

Background: Oral lichen planus (OLP) is a relatively common chronic inflammatory muco-cutaneous disease classified among the potentially malignant lesions of oral mucosa. In this study we evaluated tissue expression of p₅₃، ki-67 in OLP com­pared to normal oral mucosa.
Methods: Specimens (formalin-fixed and paraffin- embedded) of 44 lesions of OLP and 30 controls referred to immuno­histochem­istry (IHC) analysis for p₅₃ and ki-67. Results of immunohistochemistry were statistically evaluated by means of Kolmogorov- Smirnov, Kruskal- Wallis and t-test. The level of statistical significance was established at P< 0.05.
Results: This study included 44 patients with OLP (27 females and 17 males) and 30 subjects as control group (18 females and 12 males).The mean expression of p₅₃ in patients with OLP was greater than subjects in control group. The mean expres­sion of ki- 67 in patients with OLP was more than people in control group. These differences were statistically signifi­cant (P= 0.001).
Conclusion: Since p₅₃ and ki-67 extensively accepted as important biomarkers in diagnosis, prognosis and treatment of malig­nant and premalignant lesions, therefore high degree of presence of these biomarkers in chronic premalignant lesion like OLP can be a great helpful in its prognosis and suggested treatments.