Short Chain Fatty Acid Sodium Butyrate Increases miR-21, miR-143 and miR-145 Expression in Human Colorectal Cancer HCT-116 Cell Line
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Abstract
Background: Sodium butyrate (NaBu) is a short-chain fatty acid; it is one of the histone deacetylase inhibitors, which can alter both genetic and epigenetic expressions. The present study aimed to elucidate the effect of NaBu on the expression of miR-21, miR-143, and miR-145 in human colorectal cancer HCT-116 cell lines.
Methods: This study was done in Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. HCT-116 cell line was treated with diverse concentrations of NaBu (6.25 mM to 200 mM) at 24, 48, and 72 h. MTT assay was used for assessing the cytotoxicity. Quantitative Real-Time-PCR was performed to investigate the gene expression of miR-21, miR-143, and miR-145.
Results: IC50 values were evaluated by MTT assay. IC50 for HCT-116 was 50 mM, 12.5 mM, and 6.25 mM for 24, 48, and 72 h of incubation, respectively. According to the Real-Time-PCR results, 50 mM NaBu after 24 h caused a significant up-regulation in the expression of the miR-21, miR-143, and miR-145 (P<0.05). In 48 h, incubation, 12.5 mM NaBu caused a significant up-regulation in the expression of the miR-21, miR-143, and miR-145 (P<0.05). In treated cells with 6.25 mM NaBu after 72 h of incubation caused a significant up-regulation in the expression of the miR-21, miR-143, and miR-145 compared with untreated cells (P<0.05).
Conclusion: The upregulation of miR-21, miR-143, and miR-145 expression are mediated by transcriptional regulation and the activation of this miR promoter is modulated by histone acetylation. The employment of NaBu may represent a promising approach for improving HDACi drug-based therapies for colon cancers.
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26. Zhao X, Shi YQ, Yan CC, et al (2015). Up‐regulation of miR‐21 and miR‐23a Contributes to As2O3‐induced hERG Channel Deficiency. Basic Clin Pharmacol Toxicol, 116(6):516-23.
27. Hu S, Liu L, Chang EB, et al (2015). Butyrate inhibits pro-proliferative miR-92a by diminishing c-Myc-induced miR-17-92a cluster transcription in human colon cancer cells. Mol Cancer, 14: 180.
28. Liu L, Yu X, Guo X, et al (2012). miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2. Mol Med Rep, 5(3): 753-60.
29. Lu Y, Chopp M, Zheng X, et al (2013). MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncol Rep, 29(1): 67-72.
30. Sachdeva M, Mo Y-Y (2010). miR-145-mediated suppression of cell growth, invasion and metastasis. Am J Transl Res, 2(2): 170-80.
31. Zeinali T, Mansoori B, Mohammadi A, et al (2019). Regulatory mechanisms of miR-145 expression and the importance of its function in cancer metastasis. Biomed Pharmacother, 109: 195-207.
32. Kent OA, McCall MN, Cornish TC, Halushka MK (2014). Lessons from miR-143/145: the importance of cell-type localization of miRNAs. Nucleic Acids Res, 42(12): 7528-38.
33. Yang J, Zhang J-y, Chen J, et al (2014). Prognostic role of microRNA-221 in various human malignant neoplasms: a meta-analysis of 20 related studies. PLoS One, 9(1): e87606.
34. Campayo M, Navarro A, Viñolas N, et al (2013). Low miR-145 and high miR-367 are associated with unfavourable prognosis in resected nonsmall cell lung cancer. Eur Respir J, 41(5): 1172-8.
35. Ye D, Shen Z, Zhou S (2019). Function of microRNA-145 and mechanisms underlying its role in malignant tumor diagnosis and treatment. Cancer Manag Res, 11: 969-979..
36. Slaby O, Svoboda M, Fabian P, et al (2007). Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer. Oncology, 72(5-6): 397-402.
37. Bai J, Xue H, Zhang C (2016). Down-regulation of microRNA-143 is associat-ed with colorectal cancer progression. Eur Rev Med Pharmacol Sci, 20(22): 4682-4687.
2. Egger G, Liang G, Aparicio A, Jones PA (2004). Epigenetics in human disease and prospects for epigenetic therapy. Nature, 429(6990): 457-63.
3. Ross SA, Davis CD (2011). MicroRNA, nutrition, and cancer prevention. Adv Nutr, 2(6): 472-85.
4. Palmer JD, Soule BP, Simone BA, et al (2014). MicroRNA expression altered by diet: can food be medicinal? Ageing Res Rev, 17: 16-24.
5. Aslam MI, Patel M, Singh B, et al (2012). MicroRNA manipulation in colorectal cancer cells: from laboratory to clinical application. J Transl Med, 10: 128.
6. Falzone L, Scola L, Zanghì A, et al (2018). Integrated analysis of colorectal cancer microRNA datasets: Identification of microRNAs associated with tumor development. Aging (Albany NY), 10(5): 1000-1014
7. Iio A, Nakagawa Y, Hirata I, Naoe T, Akao Y (2010). Identification of non-coding RNAs embracing microRNA-143/145 cluster. Mol Cancer, 9: 136.
8. Wang Y, Lee CG (2009). MicroRNA and cancer–focus on apoptosis. J Cell Mol Med, 13(1): 12-23.
9. Dyrskjøt L, Ostenfeld MS, Bramsen JB, et al (2009). Genomic profiling of microRNAs in bladder cancer: miR-129 is associated with poor outcome and promotes cell death in vitro. Cancer Res, 69(11): 4851-60.
10. Ribas J, Ni X, Haffner M, et al (2009). miR-21: an androgen receptor–regulated microRNA that promotes hormone-dependent and hormone-independent prostate cancer growth. Cancer Res, 69(18): 7165-9.
11. Schetter AJ, Leung SY, Sohn JJ, et al (2008). MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA, 299(4): 425-36.
12. Markou A, Tsaroucha EG, Kaklamanis L, et al (2008). Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non–small cell lung cancer by quantitative real-time RT-PCR. Clin Chem, 54(10): 1696-704.
13. Kjaer-Frifeldt S, Hansen T, Nielsen B, et al (2012). The prognostic importance of miR-21 in stage II colon cancer: a population-based study. Br J Cancer, 107(7): 1169-74.
14. Klisovic M, Maghraby E, Parthun M, et al (2003). Depsipeptide (FR 901228) promotes histone acetylation, gene transcription, apoptosis and its activity is enhanced by DNA methyltransferase inhibitors in AML1/ETO-positive leukemic cells. Leukemia, 17(2): 350-8.
15. Ali SR, Humphreys KJ, McKinnon RA, Michael MZ (2015). Impact of histone deacetylase inhibitors on microRNA expression and cancer therapy: a review. Drug Dev Res, 76(6): 296-317.
16. Tan J, McKenzie C, Potamitis M, et al (2014). The role of short-chain fatty acids in health and disease. Adv Immunol, 121: 91-119.
17. Cummings J, Pomare E, Branch W, et al (1987). Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut, 28(10): 1221-7.
18. Han R, Sun Q, Wu J, et al (2016). Sodium butyrate upregulates miR-203 expression to exert anti-proliferation effect on colorectal cancer cells. Cell Physiol Biochem, 39(5): 1919-29.
19. Ghiaghi M, Forouzesh F, Rahimi H (2019). Effect of Sodium Butyrate on LHX1 mRNA Expression as a Transcription Factor of HDAC8 in Human Colorectal Cancer Cell Lines. Avicenna J Med Biotechnol, 11(4): 317-324.
20. Brenner H. Kloor M2, Pox CP3 (2014). Colorectal cancer. Lancet, 383(9927): 1490-1502.
21. Ramalingam S, Subramaniam D, Anant S (2015). Manipulating miRNA expression: a novel approach for colon cancer prevention and chemotherapy. Curr Pharmacol Rep, 1(3): 141-153.
22. Bishop KS, Xu H, Marlow G (2017). Epigenetic regulation of gene expression induced by butyrate in colorectal cancer: Involvement of microRNA. Genet Epigenet, 9: 1179237X17729900.
23. Schaefer A, Jung M, Kristiansen G, et al (2010). MicroRNAs and cancer: current state and future perspectives in urologic oncology. Urol Oncol, 28(1):4-13.
24. Byrd JC, Marcucci G, Parthun MR, et al (2005). A phase 1 and pharmacodynamic study of depsipeptide (FK228) in chronic lymphocytic leukemia and acute myeloid leukemia. Blood, 105(3):959-67.
25. Wu Y, Song Y, Xiong Y, et al (2017). MicroRNA-21 (Mir-21) promotes cell growth and invasion by repressing tumor suppressor PTEN in colorectal cancer. Cell Physiol Biochem, 43(3): 945-58.
26. Zhao X, Shi YQ, Yan CC, et al (2015). Up‐regulation of miR‐21 and miR‐23a Contributes to As2O3‐induced hERG Channel Deficiency. Basic Clin Pharmacol Toxicol, 116(6):516-23.
27. Hu S, Liu L, Chang EB, et al (2015). Butyrate inhibits pro-proliferative miR-92a by diminishing c-Myc-induced miR-17-92a cluster transcription in human colon cancer cells. Mol Cancer, 14: 180.
28. Liu L, Yu X, Guo X, et al (2012). miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2. Mol Med Rep, 5(3): 753-60.
29. Lu Y, Chopp M, Zheng X, et al (2013). MiR-145 reduces ADAM17 expression and inhibits in vitro migration and invasion of glioma cells. Oncol Rep, 29(1): 67-72.
30. Sachdeva M, Mo Y-Y (2010). miR-145-mediated suppression of cell growth, invasion and metastasis. Am J Transl Res, 2(2): 170-80.
31. Zeinali T, Mansoori B, Mohammadi A, et al (2019). Regulatory mechanisms of miR-145 expression and the importance of its function in cancer metastasis. Biomed Pharmacother, 109: 195-207.
32. Kent OA, McCall MN, Cornish TC, Halushka MK (2014). Lessons from miR-143/145: the importance of cell-type localization of miRNAs. Nucleic Acids Res, 42(12): 7528-38.
33. Yang J, Zhang J-y, Chen J, et al (2014). Prognostic role of microRNA-221 in various human malignant neoplasms: a meta-analysis of 20 related studies. PLoS One, 9(1): e87606.
34. Campayo M, Navarro A, Viñolas N, et al (2013). Low miR-145 and high miR-367 are associated with unfavourable prognosis in resected nonsmall cell lung cancer. Eur Respir J, 41(5): 1172-8.
35. Ye D, Shen Z, Zhou S (2019). Function of microRNA-145 and mechanisms underlying its role in malignant tumor diagnosis and treatment. Cancer Manag Res, 11: 969-979..
36. Slaby O, Svoboda M, Fabian P, et al (2007). Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer. Oncology, 72(5-6): 397-402.
37. Bai J, Xue H, Zhang C (2016). Down-regulation of microRNA-143 is associat-ed with colorectal cancer progression. Eur Rev Med Pharmacol Sci, 20(22): 4682-4687.
| Files | ||
| Issue | Vol 53 No 5 (2024) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v53i5.15598 | |
| Keywords | ||
| Colorectal cancer Sodium butyrate Human miR-21 Human miR-143 Human miR-145 Human HCT-116 cell | ||
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How to Cite
1.
Mohammadi P, Forouzesh F, Kouhkan F. Short Chain Fatty Acid Sodium Butyrate Increases miR-21, miR-143 and miR-145 Expression in Human Colorectal Cancer HCT-116 Cell Line. Iran J Public Health. 2024;53(5):1164-1174.
