Original Article

Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p


Background: LncRNA FGD5-AS1 regulates the pathogenesis of many human diseases. We aimed to elucidate the function of lncRNA FGD5-AS1 and the regulatory mechanism of lncRNA FGD5-AS1/miR-129-5p in myocardial ischemia-reperfusion (I/R) injury.

Methods: Myocardial I/R injury mice model and H/R treated H9c2 cells were established. RT-qPCR and Western blot analysis were used to detect the mRNA and protein expression. Cell viability was detected by MTT assay. Dual luciferase reporter assay was applied to confirm the relationship between lncRNA FGD5-AS1 and miR-129-5p.

Results: LncRNA FGD5-AS1 was upregulated in myocardial I/R injury mice models and H/R treated H9c2 cells. Functionally, knockdown of lncRNA FGD5-AS1 promoted cell viability and inhibited apoptosis in H/R treated H9c2 cells. In addition, lncRNA FGD5-AS1 directly targets miR-129-5p. Upregulation of lncRNA FGD5-AS1 weakened the protective effect of miR-129-5p on myocardial I/R injury.

Conclusion: LncRNA FGD5-AS1 aggravates myocardial I/R injury by downregulating miR-129-5p.

1. Eltzschig HK, Eckle T (2011). Ischemia and reperfusion--from mechanism to translation. Nat Med, 17: 1391-1401.
2. Wang J, Toan S, Zhou H (2020). New insights into the role of mitochondria in cardiac microvascular ischemia/reperfusion injury. Angiogenesis, 23: 299-314.
3. Smart N (2017). Prospects for improving neovascularization of the ischemic heart: Lessons from development. Microcirculation, 24.
4. Boon RA, Jae N, Holdt L, Dimmeler S (2016). Long Noncoding RNAs: From Clinical Genetics to Therapeutic Targets? J Am Coll Cardiol, 67: 1214-1226.
5. Chen YQ, Yang X, Xu W, Yan Y, Chen XM, Huang ZQ (2021). Knockdown of lncRNA TTTY15 alleviates myocardial ischemia-reperfusion injury through the miR-374a-5p/FOXO1 axis. IUBMB Life, 73: 273-285.
6. Song XL, Zhang FF, Wang WJ, et al (2020). LncRNA A2M-AS1 lessens the injury of cardiomyocytes caused by hypoxia and reoxygenation via regulating IL1R2. Genes Genomics, 42: 1431-1441.
7. Cui F, Luo P, Bai Y, Meng J (2021). Silencing of Long Non-Coding RNA FGD5-AS1 Inhibits the Progression of Non-Small Cell Lung Cancer by Regulating the miR-493-5p/DDX5 Axis. Technol Cancer Res Treat, 20: 1533033821990007.
8. Ge C, Dong J, Chu Y, Cao S, Zhang J, Wei J (2020). LncRNA FGD5-AS1 promotes tumor growth by regulating MCL1 via sponging miR-153-3p in oral cancer. Aging (Albany NY), 12: 14355-14364.
9. Yang P, Xiong W, Chen X, Liu J, Ye Z (2020). Overexpression of miR-129-5p Mitigates Sepsis-Induced Acute Lung Injury by Targeting High Mobility Group Box 1. J Surg Res, 256: 23-30.
10. Liu S, Liao Q, Xu W, Zhang Z, Yin M, Cao X (2021). MiR-129-5p Protects H9c2 Cardiac Myoblasts From Hypoxia/Reoxygenation Injury by Targeting TRPM7 and Inhibiting NLRP3 Inflammasome Activation. J Cardiovasc Pharmacol, 77: 586-593.
11. Wei Q, Zhou HY, Shi XD, Cao HY, Qin L (2019). Long Noncoding RNA NEAT1 Promotes Myocardiocyte Apoptosis and Suppresses Proliferation Through Regulation of miR-129-5p. J Cardiovasc Pharmacol, 74: 535-541.
12. Ren L, Chen S, Liu W, Hou P, Sun W, Yan H (2019). Downregulation of long non-coding RNA nuclear enriched abundant transcript 1 promotes cell proliferation and inhibits cell apoptosis by targeting miR-193a in myocardial ischemia/reperfusion injury. BMC Cardiovasc Disord, 19: 192.
13. Tong G, Wang Y, Xu C, et al (2019). Long non-coding RNA FOXD3-AS1 aggravates ischemia/reperfusion injury of cardiomyocytes through promoting autophagy. Am J Transl Res, 11: 5634-5644.
14. Li D, Jiang X, Zhang X, Cao G, Wang D, Chen Z (2019). Long noncoding RNA FGD5-AS1 promotes colorectal cancer cell proliferation, migration, and invasion through upregulating CDCA7 via sponging miR-302e. In Vitro Cell Dev Biol Anim, 55: 577-585.
15. Wu L, Zhu X, Song Z, Guo M, Liang J, Yan D (2020). FGD5-AS1 facilitates glioblastoma progression by activation of Wnt/beta-catenin signaling via regulating miR-129-5p/HNRNPK axis. Life Sci, 256: 117998.
16. Zhang XQ, Song LH, Feng SJ, Dai XM (2019). LncRNA FGD5-AS1 acts as a competing endogenous RNA for miRNA-223 to lessen oxygen-glucose deprivation and simulated reperfusion (OGD/R)-induced neurons injury. Folia Neuropathol, 57: 357-365.
17. Ma R, Chen X, Ma Y, Bai G, Li DS (2020). MiR-129-5p alleviates myocardial injury by targeting suppressor of cytokine signaling 2 after ischemia/reperfusion. Kaohsiung J Med Sci, 36: 599-606.
18. Xing J, Liu J, Liu J, Xu Z (2020). miR-129-5p ameliorates ischemia-reperfusion injury by targeting HMGB1 in myocardium. Gen Physiol Biophys, 39: 461-470.
IssueVol 51 No 10 (2022) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijph.v51i10.10986
Myocardial ischemia Reperfusion injury Cardiology

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How to Cite
Wei P, Dong Z, Lou M. Lncrna FGD5-AS1 Aggravates Myocardial Ischemia-Reperfusion Injury by Sponging Mir-129-5p. Iran J Public Health. 2022;51(10):2281-2288.