Phyto-Fabrication of Silver Nanoparticles Using Typha azerbaijanensis Aerial Part and Root Extracts
-
Amir Mirzaie
,
Farzad Badmasti
,
Hedieh Dibah
,
Shadi Hajrasouliha
,
Fatemeh Yousefi
,
Romina Andalibi
,
Aliasghar Bagheri Kashtali
,
Amir Hossein Rezaei
,
Ronak Bakhtiatri
Abstract
Background: Silver nanoparticles (AgNPs) were phyto-synthesized using Typha azerbaijanensis aerial part and root extracts, and their biological activities were investigated.
Methods: This study was conducted in the Science and Research Branch, Islamic Azad University, Tehran, Iran in 2019. In this experimental study, silver nanoparticles (AgNPs) were phyto-synthesized and the physicochemical properties of AgNPs were determined using UV-Vis (UV-Vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Antibacterial and anticancer activity of synthesized AgNPs was determined using microdilution assay, and MTT 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) methods, respectively. The apoptotic effects of AgNPs were investigated using Real-Time PCR and flow cytometry techniques.
Results: Morphological analysis of the synthesized AgNPs confirmed the spherical shape of AgNPs with an average size of 10.67 to 16.69 nm. The FTIR spectrum confirmed the presence of phytochemicals from T. azerbayenensis extract at the AgNP surface. Antibacterial experiments showed that phyto-fabricated AgNPs had significant antibacterial activity against Gram-negative bacteria. The AgNPs were significantly cytotoxic against breast cancer cell line (MCF-7) through induction of apoptosis.
Conclusion: The phyto-synthesized AgNPs had biological activities could be useful in pharmaceutical applications.
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7. Sharma VK, Yngard RA, Lin Y (2009). Sil-ver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid In-terface Sci, 145: 83-96.
8. Dehghanizade S, Arasteh J, Mirzaie A (2018). Green synthesis of silver nano-particles using Anthemis atropatana extract: characterization and in vitro biological ac-tivities. Artif Cells Nanomed Biotechnol, 46(1):160-168.
9. Wintachai P, Paosen S, Yupanqui CT, Vo-ravuthikunchai SP (2019). Silver nanopar-ticles synthesized with Eucalyptus critriodora ethanol leaf extract stimulate antibacterial activity against clinically multidrug-resistant Acinetobacter baumannii isolated from pneumonia patients. Microb Pathog, 126: 245-257.
10. Arya A, Gupta K, Chundawat TS, Vaya D (2018). Biogenic synthesis of copper and silver nanoparticles using green alga Botry-ococcus brauniiand: its antimicrobial activity. Bioinorg Chem Appl, 2018:7879403.
11. Sharma V, Kaushik S, Pandit P, et al (2019). Green synthesis of silver nanoparticles from medicinal plants and evaluation of their antiviral potential against chikungu-nya virus. Appl Microbiol Biotechnol, 103(2):881-891.
12. Iqbal MJ, Ali S, Rashid U, et al (2018). Bio-synthesis of silver nanoparticles from leaf extract of Litchi chinensis and its dynamic biological impact on microbial cells and human cancer cell lines. Cell Mol Biol (Noisy-le-grand), 64(13):42-47.
13. Hamdi SMM, Assadi M (2003). Flora of Iran (Assadi et al.) 42: 13–15, Typha azer-baijanensis.
14. Nazemiyeh H, Bahadori F, Delazar A, et al (2008). Antioxidant phenolic compounds from the leaves of Erica Arborea (Erica-ceae). Nat Prod Res, 22:1385-92.
15. Elemike EE, Fayemi OE, Ekennia AC, et al (2017). Silver nanoparticles mediated by Costus afer leaf extract: synthesis, antibacte-rial, antioxidant and electrochemical properties. Molecules, 22 (5): 701.
16. Goodarzi V, Zamani H, Bajuli L, Mo-radshahi A (2014). Evaluation of antioxi-dant potential and reduction capacity of some plant extracts in silver nanoparti-cles' synthesis. Mol Biol Res Commun, 3(3):165-174.
17. Shadvar P, Mirzaie A, Yazdani S (2022). Fab-rication and optimization of amoxicillin-loaded niosomes: An appropriate strategy to increase antimicrobial and anti-biofilm effects against multidrug-resistant strains of Staphylococcus aureus. Drug Dev Ind Pharm, 1-10.doi: 10.1080/03639045.2022.2027958.
18. Gharaghie TP, Beiranvand A, Riahi A, et al (2022). Fabrication and characterization of thymol‐loaded chitosan nanogels: im-proved antibacterial and anti‐biofilm ac-tivities with negligible cytotoxicity. Chem Biodivers, 19(3):e202100426.
19. Haghighi SM, Tafvizi F, Mirzaie A (2020). Encapsulation of artemisia scoparia ex-tract in chitosan-myristate nanogel with enhanced cytotoxicity and apoptosis against hepatocellular carcinoma cell line (Huh-7). Ind Crops Prod, 155:112790.
20. Rasheed T, Bilal M, Li C, Iqbal HMN (2017). Biomedical potentialities of taraxa-cum officinale-based nanoparticles bio-synthesized using methanolic leaf extract. Curr Pharm Biotechnol, 18:1116-1123.
21. Ali MA, Mosa KA, El-Keblawy A, Alawadhi H (2019). Exogenous production of sil-ver nanoparticles by Tephrosia apollinea liv-ing plants under drought stress and their antimicrobial activities. Nanomaterials (Ba-sel), 9(12):1716.
22. Rajput S, Kumar D, Agrawal V (2020). Green synthesis of silver nanoparticles using Indian Belladonna extract and their potential antioxidant, anti-inflammatory, anticancer and larvicidal activities. Plant Cell Rep, 39(7):921-939.
23. Wu Z, Zhou W, Deng W, et al (2020). Anti-bacterial and hemostatic thiol-modified chitosan immobilized AgNPs composite sponges. ACS Appl Mater Interfaces, 12(18):20307-20320.
24. Targhia AA, Moammeri A, Jamshidifar E, et al (2021). Synergistic effect of curcumin-Cu and curcumin-Ag nanoparticle loaded niosome: Enhanced antibacterial and an-ti-biofilm activities. Bioorg Chem, 115: 105116.
25. Huq MA (2020). Green synthesis of silver nanoparticles using Pseudoduganella eburnea MAHUQ-39 and their antimicrobial mechanisms investigation against drug resistant human pathogens. Int J Mol Sci, 21(4):1510.
26. Sondi I, Salopek-Sondi B (2004). Silver na-noparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci, 275: 177-82.
27. Docea AO, Calina D, Buga AM, et al (2020). The Effect of silver nanoparticles on an-tioxidant/Pro-oxidant balance in a mu-rine model. Int J Mol Sci, 21(4):1233.
28. Shurpik DN, Sevastyanov DA, Zelenikhin PV, et al (2020). Nanoparticles based on the zwitterionic pillar(5)arene and Ag+: synthesis, self-assembly and cytotoxicity in the human lung cancer cell line A549. Beilstein J Nanotechnol, 11:421-431.
29. Bin-Jumah M, Al-Abdan M, Albasher G, Alarifi S (2020). Effects of green silver nanoparticles on apoptosis and oxidative stress in normal and cancerous human hepatic cells in vitro. Int J Nanomedicine, 15: 1537-1548.
2. Amale FR, Ferdowsian S, Hajrasouliha S, et al (2021). Gold nanoparticles loaded into niosomes: A novel approach for en-hanced antitumor activity against human ovarian cancer. Adv Powder Technol, 32(12):4711-4722.
3. Noorbazargan H, Amintehrani S, Do-latabadi A, Mashayekhi A (2021). Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells. AMB Express, 11:61.
4. Ovais M, Khalil AT, Raza A, et al (2016). Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics. Nanomedicine (Lond), 11:3157-3177.
5. Behdad R, Pargol M, Mirzaie A, et al (2020). Efflux pump inhibitory activity of biolog-ically synthesized silver nanoparticles against multidrug‐resistant Acinetobacter baumannii clinical isolates. J Basic Microbiol, 60: 494-507.
6. Ayromlou A, Masoudi S, Mirzaie A (209). Scorzonera calyculata aerial part extract me-diated synthesis of silver nanoparticles: Evaluation of their antibacterial, antioxi-dant and anticancer activities. J Clust Sci, 30: 1037-1050.
7. Sharma VK, Yngard RA, Lin Y (2009). Sil-ver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid In-terface Sci, 145: 83-96.
8. Dehghanizade S, Arasteh J, Mirzaie A (2018). Green synthesis of silver nano-particles using Anthemis atropatana extract: characterization and in vitro biological ac-tivities. Artif Cells Nanomed Biotechnol, 46(1):160-168.
9. Wintachai P, Paosen S, Yupanqui CT, Vo-ravuthikunchai SP (2019). Silver nanopar-ticles synthesized with Eucalyptus critriodora ethanol leaf extract stimulate antibacterial activity against clinically multidrug-resistant Acinetobacter baumannii isolated from pneumonia patients. Microb Pathog, 126: 245-257.
10. Arya A, Gupta K, Chundawat TS, Vaya D (2018). Biogenic synthesis of copper and silver nanoparticles using green alga Botry-ococcus brauniiand: its antimicrobial activity. Bioinorg Chem Appl, 2018:7879403.
11. Sharma V, Kaushik S, Pandit P, et al (2019). Green synthesis of silver nanoparticles from medicinal plants and evaluation of their antiviral potential against chikungu-nya virus. Appl Microbiol Biotechnol, 103(2):881-891.
12. Iqbal MJ, Ali S, Rashid U, et al (2018). Bio-synthesis of silver nanoparticles from leaf extract of Litchi chinensis and its dynamic biological impact on microbial cells and human cancer cell lines. Cell Mol Biol (Noisy-le-grand), 64(13):42-47.
13. Hamdi SMM, Assadi M (2003). Flora of Iran (Assadi et al.) 42: 13–15, Typha azer-baijanensis.
14. Nazemiyeh H, Bahadori F, Delazar A, et al (2008). Antioxidant phenolic compounds from the leaves of Erica Arborea (Erica-ceae). Nat Prod Res, 22:1385-92.
15. Elemike EE, Fayemi OE, Ekennia AC, et al (2017). Silver nanoparticles mediated by Costus afer leaf extract: synthesis, antibacte-rial, antioxidant and electrochemical properties. Molecules, 22 (5): 701.
16. Goodarzi V, Zamani H, Bajuli L, Mo-radshahi A (2014). Evaluation of antioxi-dant potential and reduction capacity of some plant extracts in silver nanoparti-cles' synthesis. Mol Biol Res Commun, 3(3):165-174.
17. Shadvar P, Mirzaie A, Yazdani S (2022). Fab-rication and optimization of amoxicillin-loaded niosomes: An appropriate strategy to increase antimicrobial and anti-biofilm effects against multidrug-resistant strains of Staphylococcus aureus. Drug Dev Ind Pharm, 1-10.doi: 10.1080/03639045.2022.2027958.
18. Gharaghie TP, Beiranvand A, Riahi A, et al (2022). Fabrication and characterization of thymol‐loaded chitosan nanogels: im-proved antibacterial and anti‐biofilm ac-tivities with negligible cytotoxicity. Chem Biodivers, 19(3):e202100426.
19. Haghighi SM, Tafvizi F, Mirzaie A (2020). Encapsulation of artemisia scoparia ex-tract in chitosan-myristate nanogel with enhanced cytotoxicity and apoptosis against hepatocellular carcinoma cell line (Huh-7). Ind Crops Prod, 155:112790.
20. Rasheed T, Bilal M, Li C, Iqbal HMN (2017). Biomedical potentialities of taraxa-cum officinale-based nanoparticles bio-synthesized using methanolic leaf extract. Curr Pharm Biotechnol, 18:1116-1123.
21. Ali MA, Mosa KA, El-Keblawy A, Alawadhi H (2019). Exogenous production of sil-ver nanoparticles by Tephrosia apollinea liv-ing plants under drought stress and their antimicrobial activities. Nanomaterials (Ba-sel), 9(12):1716.
22. Rajput S, Kumar D, Agrawal V (2020). Green synthesis of silver nanoparticles using Indian Belladonna extract and their potential antioxidant, anti-inflammatory, anticancer and larvicidal activities. Plant Cell Rep, 39(7):921-939.
23. Wu Z, Zhou W, Deng W, et al (2020). Anti-bacterial and hemostatic thiol-modified chitosan immobilized AgNPs composite sponges. ACS Appl Mater Interfaces, 12(18):20307-20320.
24. Targhia AA, Moammeri A, Jamshidifar E, et al (2021). Synergistic effect of curcumin-Cu and curcumin-Ag nanoparticle loaded niosome: Enhanced antibacterial and an-ti-biofilm activities. Bioorg Chem, 115: 105116.
25. Huq MA (2020). Green synthesis of silver nanoparticles using Pseudoduganella eburnea MAHUQ-39 and their antimicrobial mechanisms investigation against drug resistant human pathogens. Int J Mol Sci, 21(4):1510.
26. Sondi I, Salopek-Sondi B (2004). Silver na-noparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci, 275: 177-82.
27. Docea AO, Calina D, Buga AM, et al (2020). The Effect of silver nanoparticles on an-tioxidant/Pro-oxidant balance in a mu-rine model. Int J Mol Sci, 21(4):1233.
28. Shurpik DN, Sevastyanov DA, Zelenikhin PV, et al (2020). Nanoparticles based on the zwitterionic pillar(5)arene and Ag+: synthesis, self-assembly and cytotoxicity in the human lung cancer cell line A549. Beilstein J Nanotechnol, 11:421-431.
29. Bin-Jumah M, Al-Abdan M, Albasher G, Alarifi S (2020). Effects of green silver nanoparticles on apoptosis and oxidative stress in normal and cancerous human hepatic cells in vitro. Int J Nanomedicine, 15: 1537-1548.
| Files | ||
| Issue | Vol 51 No 5 (2022) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v51i5.9425 | |
| Keywords | ||
| Typha azerbaijanensis Silver nanoparticle Antibacterial Anticancer Antioxidant Apoptosis | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Mirzaie A, Badmasti F, Dibah H, Hajrasouliha S, Yousefi F, Andalibi R, Bagheri Kashtali A, Rezaei AH, Bakhtiatri R. Phyto-Fabrication of Silver Nanoparticles Using Typha azerbaijanensis Aerial Part and Root Extracts. Iran J Public Health. 2022;51(5):1097-1106.
