Polymorphisms of Fatty Acid Elongase 2 Gene Afftects Risk of Pulmonary Tuberculosis in China Han Population
-
Min Mu
,
Li Jing
,
Yuan-Jie Zou
,
Xing-Rong Tao
,
Fei Wang
,
Jun Lu
Abstract
Abstract
Background: As an infectious disease closely related to Mycobacterium tuberculosis, autoimmunity, inflammation, environment and heredity, the relationship between the single nucleotide polymorphism of elongase 2 gene and the susceptibility to tuberculosis is still unknown.
Methods: Between January 2016 and November 2018, a hospital-based case-control study was conducted. This epidemiological survey was conducted in both hospitals every three months. rs3798719, rs1570069, and rs2236212 in ELOVL2 gene were detected by Sanger sequencing.
Results: Stratified by gender, the genotypes and allele frequencies of rs3798719, rs1570069 and rs2236212 showed significant differences between the two groups (χ2 = 6.987, P = 0.030), Genetic modeling showed that rs3798719 was statistically different in the overdominance model (χ2 = 4.784, OR = 1.414, 95% CI: 1.036-1.929, P < 0.05). The polymorphism of rs2236212 between male TB patients and healthy controls was statistically different in the dominance model. (χ2 = 4.192, OR = 0.507; 95% CI: 0.262-0.981, P < 0.05).
Conclusion: The rs3798719 of ELOVL2 gene may be associated with susceptibility to TB in female population and the rs2236212 of ELOVL2 gene may be associated with TB incidence in male patients.
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3. Wang W, Deng G, Zhang G, et al (2019). Genetic polymorphism rs8193036 of IL17A is associated with increased sus-ceptibility to pulmonary tuberculosis in Chinese Han population. Cytokine, 127:154956.
4. Simopoulos AP (2004). Omega-6 /omega-3 essential fatty acid ratio and chronic dis-eases. Food Rev Int, 20:77-90.
5. Simopoulos AP (2002). The importance of the ratio of omega-6 /omega-3 essential fatty acids. Biomed Pharmacother, 56:365-379.
6. Hibbeln JR, Nieminen LR, Blasbalg TL, et al (2006). Healthy intakes of n-3 and n-6 fatty acids: estimations considering worldwide diversity. Am J Clin Nutr, 83:1483S-1493S.
7. Soh AZ, Chee CB, Wang YT, et al (2016). Dietary Cholesterol Increases the Risk whereas PUFAs Reduce the Risk of Ac-tive Tuberculosis in Singapore Chinese. J Nutr, 146:1093-1100.
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9. Wu Y, Wang Y, Tian H, et al (2019). DHA intake interacts with ELOVL2 and ELOVL5 genetic variants to influence polyunsaturated fatty acids in human milk. J Lipid Res, 60:1043-1049.
10. Li X, Gan ZW, Ding Z, et al (2017). Genetic Variants in the ELOVL5 but not ELOVL2 Gene Associated with Polyun-saturated Fatty Acids in Han Chinese Breast Milk. Biomed Environ Sci, 30:64-67.
11. Barman M, Nilsson S, Torinsson NÅ, et al (2015). Single Nucleotide Polymorphisms in the FADS Gene Cluster but not the ELOVL2 Gene are Associated with Se-rum Polyunsaturated Fatty Acid Compo-sition and Development of Allergy (in a Swedish Birth Cohort). Nutrients, 7:10100-10115.
12. Illig T, Gieger C, Zhai G, et al (2010). A ge-nome-wide perspective of genetic varia-tion in human metabolism. Nat Genet, 42:137-141.
13. Lemaitre RN, Tanaka T, Tang W, et al (2011). Genetic loci associated with plas-ma phospholipid n-3 fatty acids: a meta-analysis of genome-wide association studies from the CHARGE Consortium. PLoS Genet, 7:e1002193.
14. Mu M, Wang SF, Sheng J, et al (2014). Die-tary Patterns Are Associated with Body Mass Index and Bone Mineral Density in Chinese Freshmen. J Am Coll Nutr, 32:120-128.
15. Ministry of Health (2012). Diagnostic criteria for pulmonary tuberculosis. Ministry of Health, People’s Republic of China; 2008.
16. Macfarlane DJ, Lee CC, Ho EY, et al (2007). Reliability and validity of the Chinese ver-sion of IPAQ (short, last 7 days). J Sci Med Sport, 10:45–51.
17. Datsomor AK, Zic N, Li K, et al (2019). CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon inhibits elonga-tion of polyunsaturated fatty acids and induces Srebp-1 and target genes. Sci Rep, 9:533.
18. Pauter AM, Olsson P, Asadi A, et al (2014). Elovl2 ablation demonstrates that sys-temic DHA is endogenously produced and is essential for lipid homeostasis in mice. J Lipid Res, 55:718-728.
19. Sun C, Zou M, Wang X, et al (2018). FADS1- FADS2 and ELOVL2 gene pol-ymorphisms in susceptibility to autism spectrum disordersin Chinese children. BMC Psychiatry, 18:283.
20. Pauter AM,-Trattner S, Gonzalez-Bengtsson A, et al (2017). Both maternal and off-spring Elovl2 genotypes determine sys-temic DHA levels in perinatal mice. J Li-pid Res, 58:111-123.
21. Hu Y, Li H, Lu L, et al (2016). Genome-wide meta-analyses identify novel loci as-sociated with n-3 and n-6 polyunsaturat-ed fatty acid levels in Chinese and Euro-pean-ancestry populations. Hum Mol Genet, 25:1215-1224.
22. Cormier H, Rudkowska I, Lemieux S, et al (2014). Effects of FADS and ELOVL polymorphisms on indexes of desaturase and elongase activities: results from a pre-post fish oil supplementation. Genes Nutr, 9:437.
23. Morales E, Bustamante M, Gonzalez JR, et al (2011). Genetic variants of the FADS gene cluster and ELOVL gene family, co-lostrums LC-PUFA levels, breastfeeding, and child cognition. PLoS One, 6: e17181.
24. McFarland CT, Fan YY, Chapkin RS, et al (2008). Dietary polyunsaturated fatty acids modulate resistance to Mycobacterium tuberculosis in guinea pigs. J Nutr, 138: 2123-2128.
25. Jordao L, Lengeling A, Bordat Y, et al (2008). Effects of omega-3 and -6 fatty acids on Mycobacterium tuberculosis in mac-rophages and in mice. Microbes Infect, 10:1379-1386.
26. Anes E, Kühnel MP, Bos E, et al (2003). Se-lected lipids activate phagosome actin as-sembly and maturation resulting in killing of pathogenic mycobacteria. Nat Cell Biol, 5:793-802.
27. Bonilla DL, Ly LH, Fan YY, et al (2010). In-corporation of a dietary omega 3 fatty ac-id impairs murine macrophage responses to Mycobacterium tuberculosis. PLoS One, 5:e10878.
28. Paul KP, Leichsenring M, Pfisterer M, et al (1997). Influence of n-6 and n-3 polyun-saturated fatty acids on the resistance to experimental tuberculosis. Metabolism, 46:619-624.
29. Gutierrez MG, Gonzalez AP, Anes E, et al (2009). Role of lipids in killing mycobac-teria by macrophages: evidence for NF-kappa B-dependent and -independent killing induced by different lipids. Cell Mi-crobiol, 11:406-420.
30. Radzikowska U, Rinaldi AO, Çelebi Sözener Z, et al (2019).The Influence of Dietary Fatty Acids on Immune Responses. Nu-trients, 11: pii: E2990..
31. Decoeur F, Benmamar-Badel A, Leyrolle Q, et al (2020). Dietary N-3 PUFA deficiency affects sleep-wake activity in basal condi-tion and in response to an inflammatory challenge in mice. Brain Behav Immun, 85:162-169.
32. Magrum LJ, Johnston PV (1983). Modula-tion of prostaglandin synthesis in rat per-itoneal macrophages with ω-3 fatty acids. Lipids, 18:514-521.
33. Schroit AJ, Gallily R (1979). Macrophage fat-ty acid composition and phagocytosis: ef-fect of unsaturation on cellular phagocytic activity. Immunology, 36:199-205.
34. Rodrigues FG, Campos JB, Silva GD, et al (2016). Endoscopic ultrasound in the di-agnosis of foreign bodies of the colon and rectum. Rev Assoc Med Bras, 62: 818-821.
35. Serhan CN (2014). Pro-resolving lipid medi-ators are leads for resolution physiology. Nature, 510:92-101.
36. Serhan CN, Chiang N, Dalli J, et al (2014). Lipid mediators in the resolution of in-flammation. Cold Spring Harb Perspect Biol, 7:a016311.
37. Williams-Bey Y, Boularan C, Vural A, et al (2014). Omega-3 free fatty acids suppress macrophage inflammasome activation by inhibiting NF-κB activation and enhanc-ing autophagy. PLoS One, 9: e97957. .
38. Li H, Ruan XZ, Powis SH, et al (2005). EPA and DHA reduce LPS-induced inflamma-tion responses in HK-2 cells: evidence for a PPAR-gamma-dependent mecha-nism. Kidney Int, 67: 867-874.
39. Zheng Z, Ge Y, Zhang J, et al (2015). PUFA diets alter the microRNA expression pro-files in an inflammation rat model. Mol Med Rep, 11: 4149-4157.
40. Camuesco D, Gálvez J, Nieto A, et al (2005). Dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyun-saturated fatty acids, attenuates colonic in-flammation in rats with DSS-induced co-litis. J Nutr, 135: 687-694.
41. Kelley DS, Siegel D, Fedor DM, et al (2009). DHA supplementation decreases serum C-reactive protein and other markers of inflammation in hypertriglyceridemic men. J Nutr, 139:495-501.
2. Möller M, Hoal EG (2010). Past, present and future directions in human genetic susceptibility to tuberculosis. FEMS Im-munol Med Microbiol, 58:3-26.
3. Wang W, Deng G, Zhang G, et al (2019). Genetic polymorphism rs8193036 of IL17A is associated with increased sus-ceptibility to pulmonary tuberculosis in Chinese Han population. Cytokine, 127:154956.
4. Simopoulos AP (2004). Omega-6 /omega-3 essential fatty acid ratio and chronic dis-eases. Food Rev Int, 20:77-90.
5. Simopoulos AP (2002). The importance of the ratio of omega-6 /omega-3 essential fatty acids. Biomed Pharmacother, 56:365-379.
6. Hibbeln JR, Nieminen LR, Blasbalg TL, et al (2006). Healthy intakes of n-3 and n-6 fatty acids: estimations considering worldwide diversity. Am J Clin Nutr, 83:1483S-1493S.
7. Soh AZ, Chee CB, Wang YT, et al (2016). Dietary Cholesterol Increases the Risk whereas PUFAs Reduce the Risk of Ac-tive Tuberculosis in Singapore Chinese. J Nutr, 146:1093-1100.
8. Mateusz C, Monika, T, Michał T (2018).A Comprehensive Review of Chemistry, Sources and Bioavailability of Omega-3 Fatty Acids. Nutrients, 10:pii: E1662.
9. Wu Y, Wang Y, Tian H, et al (2019). DHA intake interacts with ELOVL2 and ELOVL5 genetic variants to influence polyunsaturated fatty acids in human milk. J Lipid Res, 60:1043-1049.
10. Li X, Gan ZW, Ding Z, et al (2017). Genetic Variants in the ELOVL5 but not ELOVL2 Gene Associated with Polyun-saturated Fatty Acids in Han Chinese Breast Milk. Biomed Environ Sci, 30:64-67.
11. Barman M, Nilsson S, Torinsson NÅ, et al (2015). Single Nucleotide Polymorphisms in the FADS Gene Cluster but not the ELOVL2 Gene are Associated with Se-rum Polyunsaturated Fatty Acid Compo-sition and Development of Allergy (in a Swedish Birth Cohort). Nutrients, 7:10100-10115.
12. Illig T, Gieger C, Zhai G, et al (2010). A ge-nome-wide perspective of genetic varia-tion in human metabolism. Nat Genet, 42:137-141.
13. Lemaitre RN, Tanaka T, Tang W, et al (2011). Genetic loci associated with plas-ma phospholipid n-3 fatty acids: a meta-analysis of genome-wide association studies from the CHARGE Consortium. PLoS Genet, 7:e1002193.
14. Mu M, Wang SF, Sheng J, et al (2014). Die-tary Patterns Are Associated with Body Mass Index and Bone Mineral Density in Chinese Freshmen. J Am Coll Nutr, 32:120-128.
15. Ministry of Health (2012). Diagnostic criteria for pulmonary tuberculosis. Ministry of Health, People’s Republic of China; 2008.
16. Macfarlane DJ, Lee CC, Ho EY, et al (2007). Reliability and validity of the Chinese ver-sion of IPAQ (short, last 7 days). J Sci Med Sport, 10:45–51.
17. Datsomor AK, Zic N, Li K, et al (2019). CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon inhibits elonga-tion of polyunsaturated fatty acids and induces Srebp-1 and target genes. Sci Rep, 9:533.
18. Pauter AM, Olsson P, Asadi A, et al (2014). Elovl2 ablation demonstrates that sys-temic DHA is endogenously produced and is essential for lipid homeostasis in mice. J Lipid Res, 55:718-728.
19. Sun C, Zou M, Wang X, et al (2018). FADS1- FADS2 and ELOVL2 gene pol-ymorphisms in susceptibility to autism spectrum disordersin Chinese children. BMC Psychiatry, 18:283.
20. Pauter AM,-Trattner S, Gonzalez-Bengtsson A, et al (2017). Both maternal and off-spring Elovl2 genotypes determine sys-temic DHA levels in perinatal mice. J Li-pid Res, 58:111-123.
21. Hu Y, Li H, Lu L, et al (2016). Genome-wide meta-analyses identify novel loci as-sociated with n-3 and n-6 polyunsaturat-ed fatty acid levels in Chinese and Euro-pean-ancestry populations. Hum Mol Genet, 25:1215-1224.
22. Cormier H, Rudkowska I, Lemieux S, et al (2014). Effects of FADS and ELOVL polymorphisms on indexes of desaturase and elongase activities: results from a pre-post fish oil supplementation. Genes Nutr, 9:437.
23. Morales E, Bustamante M, Gonzalez JR, et al (2011). Genetic variants of the FADS gene cluster and ELOVL gene family, co-lostrums LC-PUFA levels, breastfeeding, and child cognition. PLoS One, 6: e17181.
24. McFarland CT, Fan YY, Chapkin RS, et al (2008). Dietary polyunsaturated fatty acids modulate resistance to Mycobacterium tuberculosis in guinea pigs. J Nutr, 138: 2123-2128.
25. Jordao L, Lengeling A, Bordat Y, et al (2008). Effects of omega-3 and -6 fatty acids on Mycobacterium tuberculosis in mac-rophages and in mice. Microbes Infect, 10:1379-1386.
26. Anes E, Kühnel MP, Bos E, et al (2003). Se-lected lipids activate phagosome actin as-sembly and maturation resulting in killing of pathogenic mycobacteria. Nat Cell Biol, 5:793-802.
27. Bonilla DL, Ly LH, Fan YY, et al (2010). In-corporation of a dietary omega 3 fatty ac-id impairs murine macrophage responses to Mycobacterium tuberculosis. PLoS One, 5:e10878.
28. Paul KP, Leichsenring M, Pfisterer M, et al (1997). Influence of n-6 and n-3 polyun-saturated fatty acids on the resistance to experimental tuberculosis. Metabolism, 46:619-624.
29. Gutierrez MG, Gonzalez AP, Anes E, et al (2009). Role of lipids in killing mycobac-teria by macrophages: evidence for NF-kappa B-dependent and -independent killing induced by different lipids. Cell Mi-crobiol, 11:406-420.
30. Radzikowska U, Rinaldi AO, Çelebi Sözener Z, et al (2019).The Influence of Dietary Fatty Acids on Immune Responses. Nu-trients, 11: pii: E2990..
31. Decoeur F, Benmamar-Badel A, Leyrolle Q, et al (2020). Dietary N-3 PUFA deficiency affects sleep-wake activity in basal condi-tion and in response to an inflammatory challenge in mice. Brain Behav Immun, 85:162-169.
32. Magrum LJ, Johnston PV (1983). Modula-tion of prostaglandin synthesis in rat per-itoneal macrophages with ω-3 fatty acids. Lipids, 18:514-521.
33. Schroit AJ, Gallily R (1979). Macrophage fat-ty acid composition and phagocytosis: ef-fect of unsaturation on cellular phagocytic activity. Immunology, 36:199-205.
34. Rodrigues FG, Campos JB, Silva GD, et al (2016). Endoscopic ultrasound in the di-agnosis of foreign bodies of the colon and rectum. Rev Assoc Med Bras, 62: 818-821.
35. Serhan CN (2014). Pro-resolving lipid medi-ators are leads for resolution physiology. Nature, 510:92-101.
36. Serhan CN, Chiang N, Dalli J, et al (2014). Lipid mediators in the resolution of in-flammation. Cold Spring Harb Perspect Biol, 7:a016311.
37. Williams-Bey Y, Boularan C, Vural A, et al (2014). Omega-3 free fatty acids suppress macrophage inflammasome activation by inhibiting NF-κB activation and enhanc-ing autophagy. PLoS One, 9: e97957. .
38. Li H, Ruan XZ, Powis SH, et al (2005). EPA and DHA reduce LPS-induced inflamma-tion responses in HK-2 cells: evidence for a PPAR-gamma-dependent mecha-nism. Kidney Int, 67: 867-874.
39. Zheng Z, Ge Y, Zhang J, et al (2015). PUFA diets alter the microRNA expression pro-files in an inflammation rat model. Mol Med Rep, 11: 4149-4157.
40. Camuesco D, Gálvez J, Nieto A, et al (2005). Dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyun-saturated fatty acids, attenuates colonic in-flammation in rats with DSS-induced co-litis. J Nutr, 135: 687-694.
41. Kelley DS, Siegel D, Fedor DM, et al (2009). DHA supplementation decreases serum C-reactive protein and other markers of inflammation in hypertriglyceridemic men. J Nutr, 139:495-501.
| Files | ||
| Issue | Vol 50 No 11 (2021) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v50i11.7580 | |
| Keywords | ||
| Tuberculosis; ELOVL2 gene Single nucleotide polymorphism China | ||
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How to Cite
1.
Mu M, Jing L, Zou Y-J, Tao X-R, Wang F, Lu J. Polymorphisms of Fatty Acid Elongase 2 Gene Afftects Risk of Pulmonary Tuberculosis in China Han Population. Iran J Public Health. 2021;50(11):2254-2262.
