Original Article

Clinical Application of Serum Inflammatory Factors Combined With Dynamic Detection in the Diagnosis and Treatment of Ne-onatal Sepsis

Abstract

Background: To investigate the clinical application value of the combination of the inflammatory factors and dynamic detection in the diagnosis and treatment of neonatal sepsis by detecting serum inflammatory factor C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) levels before and after treatment of neonatal infection. 

Methods: A total of 138 neonates with different degrees of infection were randomly enrolled, including 88 cases in the sepsis group and 50 cases in the virus infection group. Another 50 non-infected newborns in the same period were enrolled as the normal control group. Venous blood of all subjects for CRP, PCT, IL-6 detection, and send bacterial blood culture for sepsis and virus infection groups were collected at the same time. In the recovery period, venous blood of children in sepsis group was collected again to review CRP, PCT, IL-6, and differences in each test index of each group were compared.

Results: The serum CRP, PCT, IL-6 levels in the sepsis group were significantly higher than those in the virus infection group (all P <0.05); serum CRP, PCT, IL-6 levels in the sepsis group were significantly lower than before treatment (P <0.05); the sensitivity and accuracy of the combined detection of indicators for the diagnosis of neonatal sepsis were significantly improved.

Conclusion: The inflammatory factors CRP, PCT, and IL-6 are closely related to the occurrence and development of neonatal sepsis. Combined detection can effectively improve the diagnostic accordance rate, which is beneficial to the early diagnosis and early clinical intervention of neonatal sepsis.

1. Wang Y, Wang HL, Chen J, et al (2016). Clinical and prognostic value of com-bined measurement of cytokines and vascular cell adhesionmolecule-1 in prem-ature rupture of membranes. Int J Gynaecol Obstet, 132(1): 85-88.
2. Neunhoeffer F, Plinke S, Renk H, et al (2016). Serum Concentrations of Inter-leukin-6, Procalcitonin, and C-Reactive Protein: Discrimination of Septical Com-plications and Systemic Inflammatory Re-sponse Syndrome after Pediatric Surgery. Eur J Pediatr Surg, 26(2): 180-185.
3. Bhat YR, Lewis LE, KEV (2011). Bacterial isolates of early-onset neonatal sepsis and their antibiotic susceptibility pattern be-tween 1998 and 2004: an audit from a center in India. Ital J Pediatr, 11;37: 32.
4. Meem M, Modak JK, Mortuza R, et al (2011). Biomarkers for diagnosis of neo-natal infections: A systematic analysis of their potential as apoint-of-care diagnos-tics. J Glob Health, 1(2): 201-209.
5. Stocker M, van Herk W, El Helou S, et al (2017). Procalcitonin-guided decision making for duration of antibiotic therapy in neonates withsuspected early-onset sepsis: a multicentre, randomised con-trolled trial (NeoPIns). Lancet, 390(10097): 871-881.
6. Satar M, Ozlü F (2012). Neonatal sepsis: a continuing disease burden. Turk J Pediatr, 54(5): 449-457.
7. Stronati M, Bollani L, Maragliano R, et al (2013). Neonatal sepsis: new preventive strategies. Minerva Pediatr, 65(1): 103-110.
8. Gijtenbeek RG, Kerstjens JM, Reijneveld SA, et al (2015). RSV infection among chil-dren born moderately preterm in a community-based cohort. Eur J Pediatr, 174(4): 435-442.
9. Jia Y, Wang Y, Yu X (2017). Relationship between blood lactic acid, blood procalci-tonin, C-reactive protein and neonatal-sepsis and corresponding prognostic significance in sick children. Exp Ther Med, 14(3): 2189-2193.
10. Sayed Ahmed WA, Ahmed MR, Mohamed ML, et al (2016). Maternal serum interleu-kin-6 in the management of patients with preterm premature rupture of mem-branes. J Matern Fetal Neonatal Med, 29(19): 3162-3166.
11. Volanakis JE (2001). Human C-reactive pro-tein: expression, structure, and function. Mol Immunol, 38(2-3): 189-197.
12. Hahn WH, Song JH, Kim H, et al (2018). Is procalcitonin to C-reactive protein ratio useful for the detection of late onset ne-onatal sepsis. J Matern Fetal Neonatal Med, 31(6): 822-826.
13. Erlandsen EJ, Randers E (2000). Reference interval for serum C-reactive protein in healthy blood donors using the Dade Behring N Latex CRP mono assay. Scand J Clin Lab Invest, 60(1): 37-43.
14. Pontrelli G, De Crescenzo F, Buzzetti R, et al (2017). Accuracy of serum procalcitonin for the diagnosis of sepsis in neonates and children withsystemic inflammatory syndrome: a meta-analysis. BMC Infect Dis, 17(1): 302.
15. Becker KL, Snider R, Nylen ES (2010). Pro-calcitonin in sepsis and systemic inflam-mation: a harmful biomarker and a ther-apeutic target. Br J Pharmacol, 159(2): 253-264.
16. Ghorbani G (2009). Procalcitonin role in differential diagnosis of infection stages and non infection inflammation. Pak J Biol Sci, 12(4): 393-396.
17. Whicher J, Bienvenu J, Monneret G (2001). Procalcitonin as an acute phase marker. Ann Clin Biochem, 38(Pt 5): 483-493.
18. Fattah MA, Omer AF, Asaif S, et al (2017). Utility of cytokine, adhesion molecule and acute phase proteins in early diagnosis of neonatalsepsis. J Nat Sci Biol Med, 8(1): 32-39.
19. Maruna P, Nedelníková K, Gürlich R (2000). Physiology and genetics of procalcitonin. Physiol Res, 49 Suppl 1: S57-S61.
20. Shahkar L, Keshtkar A, Mirfazeli A, et al (2011). The role of IL-6 for predicting neonatal sepsis: a systematic review and meta-analysis. Iran J Pediatr, 21(4): 411-7.
21. Boskabadi H, Maamouri G, Tavakol Afshari J, et al (2013). Evaluation of serum inter-leukins-6, 8 and 10 levels as diagnostic markers of neonatal infection and possi-bility of mortality. Iran J Basic Med Sci, 16(12): 1232-7.
22. Ng PC, Cheng SH, Chui KM, et al (1997). Diagnosis of late onset neonatal sepsis with cytokines, adhesion molecule, and C-reactive protein in preterm very low birthweight infants. Arch Dis Child Fetal Neonatal Ed, 77(3): F221-F227.
23. Verboon-Maciolek MA, Thijsen SF, Hemels MA, et al (2006). Inflammatory mediators for the diagnosis and treatment of sepsis in early infancy. Pediatr Res, 59(3): 457-461.
24. Haasper C, Kalmbach M, Dikos GD, et al (2010). Prognostic value of procalcitonin (PCT) and/or interleukin-6 (IL-6) plasma levels after multiple trauma for the devel-opment of multi organ dysfunction syn-drome (MODS) or sepsis. Technol Health Care, 18(2): 89-100.
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IssueVol 50 No 2 (2021) QRcode
SectionOriginal Article(s)
Published2021-02-01
DOI https://doi.org/10.18502/ijph.v50i2.5347
Keywords
Sepsis Newborn C-reactive protein Procalcitonin Interleukin-6 Combined detection

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How to Cite
1.
SUN D, WANG Q, ZHANG X, ZHAO XZ, ZHANG H, LIU A. Clinical Application of Serum Inflammatory Factors Combined With Dynamic Detection in the Diagnosis and Treatment of Ne-onatal Sepsis. Iran J Public Health. 50(2):325-332.