A New Primer Pair in ITS1 Region for Molecular Studies on Echinococcus granulosus
Abstract
Background: Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. Using DNA based methods for strain /genotype characterizations of E. granulosus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primers. Methods: Using grinder method, a pure and high concentration DNA was extracted from 10 human hydatid cysts collected from Isfahan (central Iran) hospitals, and processed for PCR reaction. Results: Using DNASIS, the primers were designed in internal transcribed spacer 1 (ITS1) region, following analysis of 30 E. granulosus nucleotide sequences, extracted from gene bank. Conclusion: This new and specific E. granulosus primer which amplified DNA thoroughly can be applied for molecular studies on echinococcosis.Files | ||
Issue | Vol 36 No 1 (2007) | |
Section | Articles | |
Keywords | ||
Primer design ITS1 DNASIS |
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
HR Rahimi, EB Kia, SH Mirhendi, A Talebi, M Fasihi Harandi, N Jalali-zand, MB Rokni. A New Primer Pair in ITS1 Region for Molecular Studies on Echinococcus granulosus. Iran J Public Health. 1;36(1):45-49.