Original Article

Investigation of JC Polyomavirus (JCV) Genome in Colorectal Cancer Patients from Iran

Abstract

Background: JC polyomavirus (JCV) is an epitheliotropic and neurotropic virus that identified in relationship with some devastating complications such as progressive multifocal leukoencephalopathy (PML) and linked to colorectal cancer. The aim of current study was to identify the prevalence of JCV in colorectal cancer for the first time in Iran.

Methods: This retrospective case-control study was conducted by the hospitals affiliated to Iran University of Medical Sciences, Tehran, Iran from 2011 to 2016. Formalin-fixed paraffin-embedded (FFPE) blocks were used for DNA extraction by QIAamp® DNA FFPE Tissue Kit. The SYBER Green Real-time PCR assay performed by specific primers for JCV T-Large Ag. Melting curve analysis used for evaluation of amplification specificity. Positive control cloned in pTZ57R/T plasmid by Generay Biotechnology system.

Results: Of 157 specimens 66 were colorectal cancer by the mean age (y) ± std. deviation 59.35±14.48 and 91 healthy control by the mean age (y) ± std. deviation 57.21±14.66. All 157 specimens tested for JCV T-Large Ag gene by Real-time PCR method and we found that there was not any positive result although the melting analysis showed specificity of positive control amplification.

Conclusion: Low prevalence of JCV infection in Iranian CRC population confirmed by the current study results; there was not any JCV positive result in CRC and healthy control groups. Further studies by broader and different populations are recommended.

 

1. Takahashi K, Sekizuka T, Fukumoto H, et al (2017). Deep-sequence identification and role in virus replication of a JC virus quasispecies in patients with progressive multifocal leukoencephalopathy. J Virol, 91:e01335-01316.
2. Loutfy SA, Moneer MM, Salem SE, Emad A, Entsar A, Ibrahim LH, Mohamed E-CB (2017). Polyomavirus infections and its clinical relevance in cancer patients: A Prospective Study. J Infect Dis Epidemiol, 10:22-30.
3. Mirzaei H, Goudarzi H, Eslami G, Faghihloo E (2018). Role of viruses in gastrointestinal cancer. J Cell Physiol, 233:4000-4014.
4. Elia F, Villani S, Ambrogi F, et al (2017). JC virus infection is acquired very early in life: evidence from a longitudinal serological study. J Neurovirol, 23:99-105.
5. Ouwens J, Haaxma‐Reiche H, Verschuuren E, Timens W, Steenhuis L, Boer W, Bij W (2000). Visual symptoms after lung transplantation: a case of progressive multifocal leukoencephalopathy. Transpl Infect Dis, 2:29-32.
6. Sadeghi F, Salehi-Vaziri M, Ghodsi SM, et al (2015). Prevalence of JC polyomavirus large T antigen sequences among Iranian patients with central nervous system tumors. Arch Virol, 160:61-68.
7. Burnett-Hartman AN, Newcomb PA, Potter JD (2008). Infectious agents and colorectal cancer: a review of Helicobacter pylori, Streptococcus bovis, JC virus, and human papillomavirus. Cancer Epidemiol Biomarkers Prev, 17:2970-2979.
8. Toumi W, Ripalti A, Ricciardiello L, et al (2017). Detection of a new JCV strain of genotype A in a subpopulation of colorectal adenocarcinomas in Tunisia. New Microbiol, 40:99-106.
9. Delbue S, Comar M, Ferrante P (2017). Review on the role of the human Polyomavirus JC in the development of tumors. Infect Agents Cancer, 12:10.
10. Siegel RL, Miller KD, Fedewa SA, Ahnen DJ, Meester RG, Barzi A, Jemal A (2017). Colorectal cancer statistics, 2017. CA Cancer J Clin, 67:177-193.
11. Sinagra E, Raimondo D, Gallo E, et al (2017). JC Virus and Lung Adenocarcinoma: Fact or Myth? Anticancer Res, 37:3311-3314.
12. Barth H, Solis M, Lepiller Q, Sueur C, Soulier E, Caillard S, Stoll-Keller F, Fafi-Kremer S (2017). 45 years after the discovery of human polyomaviruses BK and JC: time to speed up the understanding of associated diseases and treatment approaches. Crit Rev Microbiol, 43:178-195.
13. Hu C, Huang Y, Su J, Wang M, Zhou Q, Zhu B (2018). Detection and analysis of variants of JC polyomavirus in urine samples from HIV-1-infected patients in China’s Zhejiang Province. J Int Med Res, 46(3):1024-1032.
14. Bononi I, Mazzoni E, Pietrobon S, et al (2018). Serum IgG antibodies from healthy subjects up to 100 years old react to JC polyomavirus. J Cell Physiol, 233(8):5513-5522.
15. Newcomb PA, Bush AC, Stoner GL, Lampe JW, Potter JD, Bigler J (2004). No evidence of an association of JC virus and colon neoplasia. Cancer Epidemiol Biomarkers Prev, 13:662-666.
16. Lundstig A, Stattin P, Persson K, Sasnauskas K, Viscidi RP, Gislefoss RE, Dillner J (2007). No excess risk for colorectal cancer among subjects seropositive for the JC polyomavirus. Int J Cancer, 121:1098-1102.
17. Enam S, Del Valle L, Lara C, Gan D-D, Ortiz-Hidalgo C, Palazzo JP, Khalili K (2002). Association of human polyomavirus JCV with colon cancer: evidence for interaction of viral T-antigen and β-catenin. Cancer Res, 62:7093-7101.
18. Hori R, Murai Y, Tsuneyama K, et al (2005). Detection of JC virus DNA sequences in colorectal cancers in Japan. Virchows Arch, 447:723-730.
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IssueVol 49 No 3 (2020) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijph.v49i3.3153
Keywords
JC virus Colorectal cancer Real-time polymerase chain reaction
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How to Cite
1.
KARBALAIE NIYA MH, KESHAVARZ M, SAFARNEZHAD TAMESHKEL F, TAHERIZADEH M, ESGHAEI M, PANAHI M, KEYVANI H. Investigation of JC Polyomavirus (JCV) Genome in Colorectal Cancer Patients from Iran. Iran J Public Health. 2020;49(3):557-562.