A Simple and Large Scale Performing Protocol for the Detection of Sarcocystis in Muscles of Some Small Mammals

Abstract

A simple special protocol using Semichon’s acetic carmine stain was applied for examination of muscles of some small mammals, naturally infected with Sarcocystis spp. using this method, infected muscles fibers with Sarcocystis could easily be differentiated from muscle tissues. This method can be applied for screening of infected muscles with Sarcocystis cysts on large number of samples for epidemiological studies, and in slaughterhouses on frozen samples and formalin preserved samples as well as fresh tissues.