Effect of Chitosan Nanoparticle from Penaeus semisulcatus Shrimp on Salmonella typhi and Listeria monocytogenes
,
Abstract
Background: After cellulose, chitin is one of the most important polymers in crustaceans, insects, and fungi. Chitosan is one of the most important derivatives of chitin, which has important characteristics including degradability, non-toxicity, and biocompatibility antimicrobial and antioxidant properties.
Methods: Chitosan was extracted from Penaeus semisulcatus shrimp using chemical methods and the degree of its austenitization was determined using a sub-red spectrophotometer and XRD. The nanoparticles were then synthesized using the ionic gelation method and analyzed through SEM. The antimicrobial effects of nanoparticles were also evaluated using antimicrobial tests on Listeria monocytogenes and Salmonella typhi.
Results: Nanoparticles have antimicrobial activity and can inhibit bacterial growth at different concentrations.
Conclusion: Chitosan nanoparticles have an inhibitory effect on Listeria monocytogenes, which is a gram-positive bacterium.
1. Chen LC, Tu YJ, Wang Y et al (2008). Characterization and photoreactivity of N-, S-, and C-doped ZnO under UV and visible light illumination. J Photochem Photobiol A Chem, 199: 170-178.
2. Chen X, Yan JK and Wu JY (2016). Characterization and antibacterial activity of silver nanoparticles prepared with a fungal exopolysaccharide in water. Food Hydrocolloids, 53: 69-74.
3. Chen YJ (2014). Microbiology and Nanotechnology: Focus on the Negative Impacts of Nanomaterials on Human Health and Environment. Sensors & Transducers, 169(4), 265.
4. Tran HV, Dai Tran L, Ba CT et al (2010). Synthesis, characterization, antibacterial and antiproliferative activities of monodisperse chitosan-based silver nanoparticles. Colloids Surf A Physicochem Eng Asp, 360: 32-40.
5. Cho J, Lin Q, Yang S, Simmons JG et al (2012). Sulfur-doped zinc oxide (ZnO) Nanostars: Synthesis and simulation of growth mechanism. Nano Research, 5: 20-26.
6. Manikandan A, Sathiyabama M (2015). Green synthesis of copper-chitosan nanoparticles and study of its antibacterial activity. J Nanomed Nanotechnol, 6: 1.
7. Zhang H, Jung, J, Zhao Y (2016). Preparation, characterization and evaluation of antibacterial activity of catechins and catechins–Zn complex loaded β-chitosan nanoparticles of different particle sizes. Carbohydr Polym, 137: 82-91.
8. Cong Y, Zhang J, Chen F, Anpo M (2007). Synthesis and characterization of nitrogen-doped TiO2 nanophotocatalyst with high visible light activity. J Phys Chem C Nanomater Interfaces, 111: 6976-6982.
9. Yien L, ZinNM, Sarwar A, Katas H (2012). Antifungal activity of chitosan nanoparticles and correlation with their physical properties. Int J Biomater, 2012:632698.
10. Dastjerdi R, Montazer M (2010). A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial properties. Colloids Surf B Biointerfaces, 79: 5-18.
11. Emami-Karvani Z, Chehrazi P (2011). Antibacterial activity of ZnO nanoparticle on gram-positive and gram-negative bacteria. Afr J Microbiol Res, 5: 1368-1373.
12. Gunalan S, Sivaraj R, Rajendran V (2012). Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. PRO NAT SCI-MATER, 22: 693-700.
13. Shinde, S. S. (2015). Antimicrobial Activity of ZnO Nanoparticles against Pathogenic Bacteria and Fungi. Sci Med Central, 3: 1033.
14. Jones N, Ray B, Ranjit KT, Manna AC (2008). Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett, 279: 71-76.
15. Raghupathi KR., Koodali RT, Manna AC (2011). Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir, 27: 4020-4028.
16. Hoseinzadeh E, Alikhani MY, Samarghandi MR et al (2014). Antimicrobial potential of synthesized zinc oxide nanoparticles against gram positive and gram negative bacteria. Desalination Water Treat, 52: 4969-4976.
17. Qi L, Xu Z, Jiang X, Hu C, Zou X (2004). Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res, 339(16), 2693-2700.
18. Mitra S, Gaur U, Ghosh PC, Maitra AN (2001). Tumour targeted delivery of encapsulated dextran–doxorubicin conjugate using chitosan nanoparticles as carrier. J Control Release, 74: 317-323.
19. Xu Y, Du Y (2003). Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. Int J Pharm, 250: 215-226.
20. Du WL, Niu SS, Xu YL et al (2009). Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions. Carbohydr Polym, 75: 385-389.
21. Shi, Z, Neoh KG, Kang ET, Wang W (2006). Antibacterial and mechanical properties of bone cement impregnated with chitosan nanoparticles. Biomaterials, 27: 2440-2449.
22. Anitha A, RaniVD, Krishna R et al (2009). Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N, O-carboxymethyl chitosan nanoparticles. Carbohydrate Polymers, 78: 672-677.
23. Wei D, Sun W, Qian W et al (2009). The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydrate Research, 344: 2375-2382.
24. Shrestha A, Hamblin MR, Kishen A (2014). Photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen. Nanomedicine, 10: 491-501.
25. Chen F, Shi Z, Neoh KG, Kang ET (2009). Antioxidant and antibacterial activities of eugenol and carvacrol‐grafted chitosan nanoparticles. Biotechnol Bioeng, 104: 30-39.
26. Xing K, Chen XG, Li YY et al (2008). Antibacterial activity of oleoyl-chitosan nanoparticles: A novel antibacterial dispersion system. Carbohydrate Polymers, 74: 114-120.
27. Piras AM, Maisetta G, Sandreschi S et al (2015). Chitosan nanoparticles loaded with the antimicrobial peptide temporin B exert a long-term antibacterial activity in vitro against clinical isolates of Staphylococcus epidermidis. Front Microbiol, 6: 372.
28. Perelshtein I, Ruderman E, Perkas N et al (2013). Chitosan and chitosan–ZnO-based complex nanoparticles: formation, characterization, and antibacterial activity. Journal of Materials Chemistry B, 1: 1968-1976.
29. Calvo P, Remunan‐Lopez C, Vila‐Jato JL, Alonso MJ (1997). Novel hydrophilic chitosan‐polyethylene oxide nanoparticles as protein carriers. J Appl Polym Sci, 63: 125-132.
30. Xing K, Chen XG, Liu CS, Cha DS, Park HJ (2009). Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets. Int J Food Microbiol, 132: 127-133.
2. Chen X, Yan JK and Wu JY (2016). Characterization and antibacterial activity of silver nanoparticles prepared with a fungal exopolysaccharide in water. Food Hydrocolloids, 53: 69-74.
3. Chen YJ (2014). Microbiology and Nanotechnology: Focus on the Negative Impacts of Nanomaterials on Human Health and Environment. Sensors & Transducers, 169(4), 265.
4. Tran HV, Dai Tran L, Ba CT et al (2010). Synthesis, characterization, antibacterial and antiproliferative activities of monodisperse chitosan-based silver nanoparticles. Colloids Surf A Physicochem Eng Asp, 360: 32-40.
5. Cho J, Lin Q, Yang S, Simmons JG et al (2012). Sulfur-doped zinc oxide (ZnO) Nanostars: Synthesis and simulation of growth mechanism. Nano Research, 5: 20-26.
6. Manikandan A, Sathiyabama M (2015). Green synthesis of copper-chitosan nanoparticles and study of its antibacterial activity. J Nanomed Nanotechnol, 6: 1.
7. Zhang H, Jung, J, Zhao Y (2016). Preparation, characterization and evaluation of antibacterial activity of catechins and catechins–Zn complex loaded β-chitosan nanoparticles of different particle sizes. Carbohydr Polym, 137: 82-91.
8. Cong Y, Zhang J, Chen F, Anpo M (2007). Synthesis and characterization of nitrogen-doped TiO2 nanophotocatalyst with high visible light activity. J Phys Chem C Nanomater Interfaces, 111: 6976-6982.
9. Yien L, ZinNM, Sarwar A, Katas H (2012). Antifungal activity of chitosan nanoparticles and correlation with their physical properties. Int J Biomater, 2012:632698.
10. Dastjerdi R, Montazer M (2010). A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial properties. Colloids Surf B Biointerfaces, 79: 5-18.
11. Emami-Karvani Z, Chehrazi P (2011). Antibacterial activity of ZnO nanoparticle on gram-positive and gram-negative bacteria. Afr J Microbiol Res, 5: 1368-1373.
12. Gunalan S, Sivaraj R, Rajendran V (2012). Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. PRO NAT SCI-MATER, 22: 693-700.
13. Shinde, S. S. (2015). Antimicrobial Activity of ZnO Nanoparticles against Pathogenic Bacteria and Fungi. Sci Med Central, 3: 1033.
14. Jones N, Ray B, Ranjit KT, Manna AC (2008). Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett, 279: 71-76.
15. Raghupathi KR., Koodali RT, Manna AC (2011). Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir, 27: 4020-4028.
16. Hoseinzadeh E, Alikhani MY, Samarghandi MR et al (2014). Antimicrobial potential of synthesized zinc oxide nanoparticles against gram positive and gram negative bacteria. Desalination Water Treat, 52: 4969-4976.
17. Qi L, Xu Z, Jiang X, Hu C, Zou X (2004). Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res, 339(16), 2693-2700.
18. Mitra S, Gaur U, Ghosh PC, Maitra AN (2001). Tumour targeted delivery of encapsulated dextran–doxorubicin conjugate using chitosan nanoparticles as carrier. J Control Release, 74: 317-323.
19. Xu Y, Du Y (2003). Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. Int J Pharm, 250: 215-226.
20. Du WL, Niu SS, Xu YL et al (2009). Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions. Carbohydr Polym, 75: 385-389.
21. Shi, Z, Neoh KG, Kang ET, Wang W (2006). Antibacterial and mechanical properties of bone cement impregnated with chitosan nanoparticles. Biomaterials, 27: 2440-2449.
22. Anitha A, RaniVD, Krishna R et al (2009). Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N, O-carboxymethyl chitosan nanoparticles. Carbohydrate Polymers, 78: 672-677.
23. Wei D, Sun W, Qian W et al (2009). The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydrate Research, 344: 2375-2382.
24. Shrestha A, Hamblin MR, Kishen A (2014). Photoactivated rose bengal functionalized chitosan nanoparticles produce antibacterial/biofilm activity and stabilize dentin-collagen. Nanomedicine, 10: 491-501.
25. Chen F, Shi Z, Neoh KG, Kang ET (2009). Antioxidant and antibacterial activities of eugenol and carvacrol‐grafted chitosan nanoparticles. Biotechnol Bioeng, 104: 30-39.
26. Xing K, Chen XG, Li YY et al (2008). Antibacterial activity of oleoyl-chitosan nanoparticles: A novel antibacterial dispersion system. Carbohydrate Polymers, 74: 114-120.
27. Piras AM, Maisetta G, Sandreschi S et al (2015). Chitosan nanoparticles loaded with the antimicrobial peptide temporin B exert a long-term antibacterial activity in vitro against clinical isolates of Staphylococcus epidermidis. Front Microbiol, 6: 372.
28. Perelshtein I, Ruderman E, Perkas N et al (2013). Chitosan and chitosan–ZnO-based complex nanoparticles: formation, characterization, and antibacterial activity. Journal of Materials Chemistry B, 1: 1968-1976.
29. Calvo P, Remunan‐Lopez C, Vila‐Jato JL, Alonso MJ (1997). Novel hydrophilic chitosan‐polyethylene oxide nanoparticles as protein carriers. J Appl Polym Sci, 63: 125-132.
30. Xing K, Chen XG, Liu CS, Cha DS, Park HJ (2009). Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets. Int J Food Microbiol, 132: 127-133.
| Files | ||
| Issue | Vol 49 No 2 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v49i2.3108 | |
| Keywords | ||
| Shrimp; Chitin; Chitosan nanoparticles; Listeria monocytogenes; Salmonella typhi | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
ALEBOUYEH S, ASSMAR M, MIRPOUR M. Effect of Chitosan Nanoparticle from Penaeus semisulcatus Shrimp on Salmonella typhi and Listeria monocytogenes. Iran J Public Health. 2020;49(2):369-376.
