Prevalence and Associated-Factors for Entamoeba gingivalis in Adolescents in Southeastern Iran by Culture and PCR, 2017

  • Maryam SHARIFI Department of Pediatric Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
  • Fatemeh JAHANIMOGHADAM 1. Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran 2. Kerman Social Determinants on Oral Health Research Center, Kerman University of Medical Sciences, Kerman, Iran
  • Zahra BABAEI 1. Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran 2. Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
  • Mohammad Ali MOHAMMADI Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran
  • Fatemeh SHARIFI Department of Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
  • Nima HATAMI Department of Endodontic, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
  • Masumeh DANESH Department of Pediatric Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
  • Parnian POURESLAMI 1. Department of Pediatric Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran 2. Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran 3. Kerman Social Determinants on Oral Health Research Center, Kerman University of Medical Sciences, Kerman, Iran
  • Hamidreza POURESLAMI Mail 1. Department of Pediatric Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran 2. Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran 3. Kerman Social Determinants on Oral Health Research Center, Kerman University of Medical Sciences, Kerman, Iran
Keywords:
Entamoeba gingivalis;, Prevalence;, Adolescents;, Associated factors;, Iran

Abstract

Abstract

Background: This study aimed to identify the prevalence and potential factors associated with Entamoeba gingivalis in adolescents in the city of Kerman, southeastern Iran, 2017.

Methods: In this descriptive cross-sectional study, 315 adolescents (mean age; 15 yr) consisting of 189 males and 126 females were randomly selected. For each adolescent, two specimens were collected for culturing and examination by polymerase chain reaction (PCR). Univariate and multivariate logistic regression models were performed to explore any association with demographic and clinical variables.

Results: The prevalence of E. gingivalis was 11.7%. Totally, 30 (15.9%) males and 7 (5.6%) females were infected with E. gingivalis. The rate of infection in males was 2.8 times higher than that in females (P<0.001). Statistical analysis identified 4 major factors including sex (OR=4.12, P<0.001), gingival index with severe inflammation (OR = 50, P<0.001), Candida spp. infection (OR=4.41, P<0.001) and decay-missing- filled teeth [DMFT (OR=3.27, P<0.001)]. In contrast to the aforementioned factors, adolescents with history of antibiotic consumption were significantly protected from E. gingivalis infection (OR= 3.24, P<0.001). Culture media detected 9.2% (n= 29), whilst PCR identified 11.4% (n= 36) of infection.

Conclusion: The present findings clearly demonstrate a positive association between E. gingivalis and distinct demographic and clinical risk determinants. Therefore, dental practitioners and health surveillance personnel should be aware of these confounding factors to rigorously detect and critically manage oral health issues in school-age children in order to prevent or at least minimize the eventual periodontal complications in later life.

 

Keywords:  Prevalence; Adolescents; Associated factors; Iran

 

 

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Published
2020-02-01
How to Cite
1.
SHARIFI M, JAHANIMOGHADAM F, BABAEI Z, MOHAMMADI MA, SHARIFI F, HATAMI N, DANESH M, POURESLAMI P, POURESLAMI H. Prevalence and Associated-Factors for Entamoeba gingivalis in Adolescents in Southeastern Iran by Culture and PCR, 2017. Iran J Public Health. 49(2):351-359.
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Original Article(s)