Mir-29c Expression in Glioma and Its Effects on Tumor Cell Proliferation and Apoptosis
Abstract
Background: To investigate the expression of microRNA-29c (miR-29c) in glioma and its effects on cell proliferation and apoptosis.
Methods: A retrospective analysis was performed on 76 glioma patients in People's Hospital of Weifang, Weifang, Shandong, China from May 2013 to June 2017 (experimental group) and 63 healthy subjects in the same period (control group). qRT-PCR was used to detect the miR-29c expression. Changes of serum miR-29c expression level and the correlation of miR-29c of glioma patients with the degree of tumor differentiation and pathological type were observed. Cells were grouped before transfection into blank group (no transfection), negative control group (transfected with miRNA NC) and experimental group (transfected with miR-29c mimics). CCK-8 assay was used to detect cell proliferation, flow cytometry to detect apoptosis.
Results: Expression of miR-29c in serum was significantly lower in experimental group than that in control group (P<0.05). The expression level of miR-29c of glioma patients increased with the degree of tumor differentiation (P<0.05). miR-29c in serum was not significantly correlated with the pathological type.
Conclusion: miR-29c could inhibit the proliferation of glioma cells and promote apoptosis. miR-29c is lowly expressed in glioma, and the overexpression of which in glioma cells can inhibit tumor cells proliferation and promote apoptosis. It may be a tumor suppressor miRNA of glioma, and the expression level of which can be used as reference for evaluating the grade of glioma. It is indicated that the abnormal expression of miR-29c may be a key factor in the occurrence and development of glioma.
2. Jin W, Xu X, Yang T, Hua Z (2000). p53 mutation, EGFR gene amplification and loss of heterozygosity on chromosome 10, 17 p in human gliomas. Chin Med J (Engl), 113: 662-666.
3. Stummer W (2015). Fluorescein in brain metastasis and glioma surgery. Acta Neurochir (Wien), 157: 2199-2200.
4. Yao YL, Ma J, Wang P, et al (2015). miR-101 acts as a tumor suppressor by targeting Kruppel-like factor 6 in glioblastoma stem cells. CNS Neurosci Ther, 21: 40-51.
5. Furnari FB, Fenton T, Bachoo RM, et al (2007). Malignant astrocytic glioma: genetics, biology, and paths to treatment. Genes Dev, 21: 2683-2710.
6. Li Z, Cai S, Liu Y, Yang C, Tian Y, Chen G, Cao C (2016). Over-expression of Gαi3 in human glioma is required for Akt-mTOR activation and cell growth. Oncotarget, DOI- 10.18632/oncotarget.10995.
7. Gebeshuber CA, Zatloukal K, Martinez J (2009). miR-29a suppresses tristetraprolin, which is a regulator of epithelial polarity and metastasis. EMBO Rep, 10: 400-405.
8. Liu N, Tang LL, Sun Y, et al (2013). MiR-29c suppresses invasion and metastasis by targeting TIAM1 in nasopharyngeal carcinoma. Cancer Lett, 329: 181-188.
9. Kriegel AJ, Liu Y, Fang Y, Ding X, Liang M (2012). The miR-29 family: genomics, cell biology, and relevance to renal and cardiovascular injury. Physiol Genomics, 44: 237-244.
10. Liu L, Ye JX, Qin YZ, Chen QH, Ge LY (2015). Evaluation of miR-29c, miR-124, miR-135a and miR-148a in predicting lymph node metastasis and tumor stage of gastric cancer. Int J Clin Exp Med, 8: 22227-22236.
11. Cristobal I, Madoz-Gurpide J, Manso R, Rojo F, Garcia-Foncillas J (2015). MiR-29c downregulation contributes to metastatic progression in colorectal cancer. Ann Oncol, 26: 2199-2200.
12. Shi C, Ren L, Sun C, et al (2017). miR-29a/b/c function as invasion suppressors for gliomas by targeting CDC42 and predict the prognosis of patients. Br J Cancer, 117: 1036-1047.
13. Song H, Zhang Y, Liu N, Wan C, Zhang D, Zhao S, Kong Y, Yuan L (2017). miR-92b regulates glioma cells proliferation, migration, invasion, and apoptosis via PTEN/Akt signaling pathway. J Physiol Biochem, 72: 201-211.
14. Gu JJ, Fan KC, Zhang JH, Chen HJ, Wang SS (2018). Suppression of microRNA-130b inhibits glioma cell proliferation and invasion, and induces apoptosis by PTEN/AKT signaling. Int J Mol Med, 41: 284-292.
15. Rio DC, Ares M, Hannon GJ, Nilsen TW (2010). Purification of RNA Using TRIzol (TRI Reagent). Cold Spring Harb Protoc, 2010: pdb. Prot 5439.
16. Wang Z (2011). The Guideline of the Design and Validation of MiRNA Mimics. Methods Mol Biol,676:211-23.
17. Zamai L, Falcieri E, Zauli G, Cataldi A, Vitale M (2011). Optimal detection of apoptosis by flow cytometry depends on cell. Cytometry, 14: 891-897.
18. Dermawan JK, Hitomi M, Silver DJ, et al (2016). Pharmacological Targeting of the Histone Chaperone Complex FACT Preferentially Eliminates Glioblastoma Stem Cells and Prolongs Survival in Preclinical Models. Cancer Res, 76: 2432-2442.
19. Du Y, Li J, Xu T, Zhou DD, Zhang L, Wang X (2017). MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling. Oncotarget, 8: 61510-61527.
20. Beischlag TV, Anderson G, Mazzoccoli G (2016). Glioma: Tryptophan Catabolite and Melatoninergic Pathways Link microRNA, 14-3- 3, Chromosome 4q35, Epigenetic Processes and other Glioma Biochemical Changes. Curr Pharm Des, 22: 1033-1048.
21. Bhat NS, Colden M, Dar AA, et al (2017). MicroRNA-720 Regulates E-cadherin-alphaE-catenin Complex and Promotes Renal Cell Carcinoma. Mol Cancer Ther, 16: 2840-2848.
22. Zhang HW, Wang EW, Li LX, Yi SH, Li LC, Xu FL, Wang DL, Wu YZ, Nian WQ (2016). A regulatory loop involving miR-29c and Sp1 elevates the TGF-beta1 mediated epithelial-to-mesenchymal transition in lung cancer. Oncotarget, 7: 85905-85916.
23. Niu M, Gao D, Wen Q, et al (2016). MiR-29c regulates the expression of miR-34c and miR-449a by targeting DNA methyltransferase 3a and 3b in nasopharyngeal carcinoma. BMC Cancer, 16: 218.
24. Xiao S, Yang Z, Qiu X, Lv R, Liu J, Wu M, Liao Y, Liu Q (2016). miR-29c contribute to glioma cells temozolomide sensitivity by targeting O6-methylguanine-DNA methyltransferases indirectely. Oncotarget, 7: 50229-50238.
25. Zhao D, Jiang X, Yao C, Zhang L, Liu H, Xia H, Wang Y (2014). Heat shock protein 47 regulated by miR-29a to enhance glioma tumor growth and invasion. J Neurooncol, 118: 39-47.
26. Fan YC, Mei PJ, Chen C, Miao FA, Zhang H, Li ZL (2013). MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis. J Neurooncol, 115: 179-188.
27. Wang Y, Li Y, Sun J, et al (2013). Tumor-suppressive effects of miR-29c on gliomas. Neuroreport, 24: 637-645.
| Files | ||
| Issue | Vol 49 No 2 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v49i2.3094 | |
| Keywords | ||
| MicroRNAs; Glioma Expression level Cell proliferation Apoptosis | ||
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