Cryopreservation of Echinococcus granulosus Protoscoleces

  • Alireza BADIRZADEH Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Saber RAEGHI Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
  • Vahid FALLAH-OMRANI 1. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Department of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Soheila ROUHANI Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Keywords:
Echinococcus granulosus, Cryopreservation, Dimethylsulfoxide (DMSO), Glycerol, Cystic hydatidosis, Iran

Abstract

Background: The main objective of the current study was to investigate on the cryopreservation of protoscoleces of Echinococcus granulosus, a causative agent of cystic hydatidosis in man.

Methods:  This study was conducted on isolated protoscoleces from hydatid cysts infected livers collected from slaughterhouse of Tehran, Iran in 2016. Viability of protoscoleces was evaluated by dye test. Cryopreservation of isolated protoscoleces in the presence of Dimethylsulfoxide (DMSO) and glycerol using a three-step cooling protocol involving an initial period at -20 °C, -80 °C and liquid nitrogen was performed.

Results: The mean viability rate of 10% DMSO and 15% glycerol were 9% and 8% respectively. The protoscoleces of Echinococcus granulosus have been successfully thawed and recovered after 6 months storage in liquid nitrogen.

Conclusion: Cryopreservation method needs to be improved for each species of helminthes and can be useful for other immunological and laboratorial studies.

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Published
2020-01-07
How to Cite
1.
BADIRZADEH A, RAEGHI S, FALLAH-OMRANI V, ROUHANI S. Cryopreservation of Echinococcus granulosus Protoscoleces. Iran J Public Health. 49(1):181-185.
Section
Short Communication(s)