Sulforaphane Modulates Cell Migration and Expression of β-Catenin and Epithelial Mesenchymal Transition Markers in Breast Cancer Cells

  • Mehdi BAGHERI Clinical Research Development Unit, Imam Hossein Hospital, Shahroud University of Medical Sciences, Shahroud, Iran
  • Mozhgan FAZLI School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  • Sara SAEEDNIA School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  • Majid GHOLAMI KHARANAGH Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  • Naghmeh AHMADIANKIA 1. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 2. Cancer Prevention Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
Keywords:
Sulforaphane;, Metastasis;, Breast cancer, EMT;, β-catenin

Abstract

Background: We aimed to assess the effect of sulforaphane (SFN) on breast cancer cell migration and also its effect on the expression of epithelial mesenchymal transition (EMT) markers and β-catenin.

Results: SFN markedly inhibited the migration of cells at concentrations of 10, 20, 30, and 40µM after 24, 48, and 72 h. At relatively, high concentrations (30, 40µM), SFN induced apoptosis. Moreover, SFN reduced the gene expression of ZEB1, fibronectin, and claudin-1 after 72 h. The expression of β-catenin revealed a time-dependent decrease at the concentration of 40 µM SFN.

Conclusion: Downregulation of EMT markers and β-catenin showed accordance with the inhibition of migration. SFN could be a promising drug candidate to reduce metastasis in breast cancer.

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Published
2020-01-07
How to Cite
1.
BAGHERI M, FAZLI M, SAEEDNIA S, GHOLAMI KHARANAGH M, AHMADIANKIA N. Sulforaphane Modulates Cell Migration and Expression of β-Catenin and Epithelial Mesenchymal Transition Markers in Breast Cancer Cells. Iran J Public Health. 49(1):77-85.
Section
Original Article(s)