Original Article

A Case-Control Study on the p73 G4A Gene Polymorphism and Susceptibility to Breast Cancer in an Iranian Population


Background: The tumor protein p73 (TP73) is a homolog of TP53 family. Ectopic p73 overexpression largely mimics p53 activities as a tumor suppressor and activates the transcription of p53-responsive genes and as a result induce apoptosis. This study aimed to investigate the association between p73 G4A polymorphism and the risk of breast cancer in a northeastern Iranian population.

Methods: This case-control study was performed on 105 patients who admitted in educational hospitals of Mashhad University of Medical Sciences, Iran during 2013-2015, with breast cancer as case group and 120 healthy women as the control group. PCR-CTPP method was used to investigate the relationship between the p73 G4A polymorphism and the risk of breast cancer.

Results: There was no significant association between the AA genotype of the p73 G4A polymorphism and breast cancer in case and control groups. Although G allele frequency was higher in the case group, the abundance of this allele between case and control groups was not statistically meaningful and, as a result, not associated with the risk of breast cancer in this study group.

Conclusion: There was no association between G4A p73 polymorphism and the risk of breast cancer in a northeastern Iranian population.

1. Downs-Holmes C, Silverman P (2011). Breast cancer: overview & updates. Nurse Pract, 36:20-26.
2. Parkin DM, Bray F, Ferlay J, Pisani P (2005). Global cancer statistics, 2002. CA Cancer J Clin, 55:74-108.
3. Eccles D, Tapper W (2010). The influence of common polymorphisms on breast cancer. Cancer Treat Res, 155:15-32.
4. Cordeiro E, Jackson TD, Elnahas A, Cil T (2014). Higher rate of breast surgery complications in patients with metastatic breast cancer: an analysis of the NSQIP database. Ann Surg Oncol, 21:3167-3172.
5. Wilson C, Tobin S, Young R (2004). The exploding worldwide cancer burden: the impact of cancer on women. Int J Gynecol Cancer, 14:1-11.
6. Torre LA, Bray F, Siegel RL et al (2015). Global cancer statistics, 2012. CA Cancer J Clin, 65:87-108.
7. Kamińska M, Ciszewski T, Łopacka-Szatan K et al (2015). Breast cancer risk factors. Prz Menopauzalny, 14:196-202.
8. Nelson HD, Zakher B, Cantor A et al (2012). Risk factors for breast cancer for women aged 40 to 49 years: A systematic review and meta-analysis. Ann Intern Med, 156:635-648.
9. Taghavi A, Fazeli Z, Vahedi M et al (2012). Increased trend of breast cancer mortality in Iran. Asian Pac J Cancer Prev, 13:367-370.
10. Mousavi SM, Gouya MM, Ramazani R et al (2009). Cancer incidence and mortality in Iran. Ann Oncol, 20:556-563.
11. Harirchi I, Ebrahimi M, Zamani N et al (2000). Breast cancer in Iran: a review of 903 case records. Public Health, 114:143-145.
12. Mittal RD, George GP, Mishra J et al (2011). Role of functional polymorphisms of P53 and P73 genes with the risk of prostate cancer in a case-control study from Northern India. Arch Med Res, 42:122-127.
13. Niwa Y, Hirose K, Matsuo K et al (2005). Association of p73 G4C14-to-A4T14 polymorphism at exon 2 and p53 Arg72Pro polymorphism with the risk of endometrial cancer in Japanese subjects. Cancer Lett, 219:183-190.
14. Schabath MB, Wu X, Wei Q et al (2006). Combined effects of the p53 and p73 polymorphisms on lung cancer risk. Cancer Epidemiol Biomarkers Prev,15:158-161.
15. Hu Z, Miao X, Ma H et al (2005). Dinucleotide polymorphism of p73 gene is associated with a reduced risk of lung cancer in a Chinese population. Int J Cancer, 114:455-460.
16. Lööf J, Pfeifer D, Adell G, Sun X-F (2009). Significance of an exon 2 G4C14-to-A4T14 polymorphism in the p73 gene on survival in rectal cancer patients with or without preoperative radiotherapy. Radiother Oncol, 92:215-220.
17. Pfeifer D, Arbman G, Sun X-F (2005). Polymorphism of the p73 gene in relation to colorectal cancer risk and survival. Carcinogenesis, 26:103-107.
18. Niwa Y, Hamajima N, Atsuta Y et al (2004). Genetic polymorphisms of p73 G4C14-to-A4T14 at exon 2 and p53 Arg72Pro and the risk of cervical cancer in Japanese. Cancer Lett, 205:55-60.
19. Hishida A, Matsuo K, Tajima K et al (2004). Polymorphisms of p53 Arg72Pro, p73 G4C14-to-A4T14 at exon 2 and p21 Ser31Arg and the risk of non-Hodgkin's lymphoma in Japanese. Leuk Lymphoma, 45:957-964.
20. Ryan B, McManus R, Daly J et al (2001). A common p73 polymorphism is associated with a reduced incidence of oesophageal carcinoma. Br J Cancer, 85:1499-503.
21. Li H, Yao L, Ouyang T et al (2007). Association of p73 G4C14-to-A4T14 (GC/AT) polymorphism with breast cancer survival. Carcinogenesis, 28:372-7.
22. Kaghad M, Bonnet H, Yang A et al (1997). Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers. Cell, 90:809-819.
23. Peters MA, Janer M, Kolb S et al (2001). Germline mutations in the p73 gene do not predispose to familial prostate-brain cancer. Prostate, 48:292-296.
24. Cai YC, Yang G-y, Nie Y et al (2000). Molecular alterations of p73 in human esophageal squamous cell carcinomas: loss of heterozygosity occurs frequently; loss of imprinting and elevation of p73 expression may be related to defective p53. Carcinogenesis, 21:683-689.
IssueVol 48 No 10 (2019) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijph.v48i10.3493
Tumor protein p73 Polymorphism Breast cancer

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
TAVAKKOL AFSHARI Z, GHOLIZADEH Z, NIKPOOR AR, TAVAKKOL AFSHARI J, GANJALI R, HOMAEI SHANDIZ F, JAMIALAHMADI K. A Case-Control Study on the p73 G4A Gene Polymorphism and Susceptibility to Breast Cancer in an Iranian Population. Iran J Public Health. 2019;48(10):1855-1860.