Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran
-
Reza ZEIDABADINEZHAD
,
Hassan VATANDOOST
,
Mohammad Reza ABAI
,
Navid DINPARAST DJADID
,
Abbasali RAZ
,
Mohammad Mahdi SEDAGHAT
,
Mohamad Ali OSHAGHI
,
Ahmad RAEISI
,
Neda ADIBI
Abstract
Background: Some mosquito species which belong to the Culex. pipiens complex are primary vectors for West Nile virus, Sindbis, Dirofilaria immitis, and many arboviruses. Knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC) gene of Cx. pipiens that is inherited, is one of the important threats for the efficacy of pyrethroids insecticides. Knockdown resistance (kdr) mutation, L1014F, is a well-defined mechanism of resistance to pyrethroids and DDT in many insect species. The aim of study was to determine the mechanisms of Insecticide resistance in this species
Methods: Specimens of Cx. pipiens, the major vector of West Nile virus, were obtained in Tehran, Iran by collecting larvae from polluted wastewater in Qarchak of Tehran. In 2016 Insecticide susceptibility tests were performed according to WHO methods with deltamethrin 0.05%. We focused on determination of this point mutation in the VGSC gene of Cx. pipiens by Real-time PCR.
Results: Our results revealed high levels of resistance to deltamethrin 0.05%. The lethal times i.e. LT50 and LT90 for deltamethrin were 2.1530 and 8.5117 h respectively. The result of Real-time PCR confirmed the presence of resistant genotype in all the members of tested population. This study is the first report on kdr genotyping of Cx. pipiens from Tehran and our results on the VGSC gene in position L1014F confirmed the TTA to TTT nucleotide change.
Conclusion: This finding will provide a clue for management of insecticide resistance in mosquito which are vectors of arboviruses and decision for replacement of novel approach for vector control.
1. Chalmers J, MacMahon S, Mancia G et al (1999). 1999 World Health Organization-International Society of Hypertension Guidelines for the management of hyper-tension. Guidelines sub-committee of the World Health Organization. Clin Exp Hy-pertens, 21(5-6):1009-60.
2. Sarkar M, Baruah I, Srivastava RB, et al (2011). High throughput approach to de-tection of knockdown resistance (kdr) mutation in mosquitoes, Culex quinquefasci-atus, based on real time PCR using single labeled hybridization probe/melting curve analysis. Pest Manag Sci, 67(2):156-61.
3. Hamer GL, Kitron UD, Brawn JD et al (2008). Culex pipiens (Diptera: Culicidae): a bridge vector of West Nile virus to hu-mans. J Med Entomol, 45 (1):125-8.
4. Burton MJ, Mellor IR, Duce IR, et al (2011). Differential resistance of insect sodium channels with kdr mutations to deltame-thrin, permethrin and DDT. Insect Biochem Mol Biol, 41(9):723-32.
5. Yanola J, Chamnanya S, Lumjuan N, et al (2015). Insecticides resistance in the Culex quinquefasciatus populations from northern Thailand and possible resistance mecha-nisms. Acta Trop,149:232-8.
6. Sarkar M, Borkotoki A, Baruah I, et al (2009). Molecular analysis of knock down resistance (kdr) mutation and distribution of kdr genotypes in a wild population of Culex quinquefasciatus from India. Trop Med Int Health, 14(9):1097-104.
7. WC Marquardt. The biology of disease vectors. Elsevier Academic Press, Burlington, Massachusetts. (1996): 595-605. 632 pp.
8. Hemingway J, Ranson H (2000). Insecticide resistance in insect vectors of human dis-ease. Annu Rev Entomol, 45:371-91.
9. Soderlund DM, Bloomquist JR (1989). Neu-rotoxic actions of pyrethroid insecticides. Annu Rev Entomol, 34:77-96.
10. Bass C, Nikou D, Donnelly MJ, et al (2007). Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a compari-son of two new high-throughput assays with existing methods. Malar J, 6:111.
11. Brengues C, Hawkes NJ, Chandre F, et al (2003). Pyrethroid and DDT cross‐resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated so-dium channel gene. Med Vet Entomol, 17(1):87-94.
12. Ranson H, Jensen B, Vulule JM, et al (2000). Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol Biol, 9(5):491-7.
13. Liew M, Pryor R, Palais R, et al (2004). Gen-otyping of single-nucleotide polymor-phisms by high-resolution melting of small amplicons. Clin Chem, 50(7):1156-64.
14. Do H, Krypuy M, Mitchell PL, et al (2008). High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin em-bedded biopsies. BMC Cancer, 8:142.
15. WHO (2014). Pesticide Evaluation Scheme. Discriminating concentrations of insecti-cide for adult mosquitoes. WHO Tech-nical Report Series 818. pp 40.
16. WHO (2016). Test procedures for insecticide re-sistance 318 monitoring in malaria vector mosqui-toes. 2nd edition. World Health Organiza-tion, Geneva. Pp 54.
17. Shayan P, Borji H, Eslami A, et al (2007). Isolation of DNA from a single Hel-minth using new developed Kit in Iran and its PCR analysis. Iran J Parasitol, 2(2):34-9.
18. Corbel V, N’guessan R, Brengues C, et al (2007). Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa. Acta Trop, 101(3):207-16.
19. Martinez Torres D, Chevillon C, Brun Bara-le A, et al (1999). Voltage-dependent Na+ channels in pyrethroid-resistant Culex pipiens L mosquitoes. Pestic Sci, 55(10):1012-20.
20. Fathian M, Vatandoost H, Moosa-Kazemi SH, et al (2014). Susceptibility of Culicidae mosquitoes to some insecticides recom-mended by WHO in a malaria endemic area of southeastern Iran. J Arthropod Borne Dis, 9(1):22-34.
21. NaseriKarimi N, Vatandoost H, Bagheri M, et al (2015). Susceptibility status of Culex pipiens against deltamethrin and DDT, Urmia County, West Azerbaijan Province, northwestern Iran. Asian Pac J Trop Dis, 5(Suppl 1): S77-S79.
22. Salim-Abadi Y, Oshaghi MA, Enayati AA et al (2016). High Insecticides Resistance in Culex pipiens (Diptera: Culicidae) from Tehran, Capital of Iran. Iran J Arthropod Borne Dis, 10(4):483-92.
23. Akiner MM, Simsek MF, Caglar SS (2009). Insecticide resistance of Culex pipiens (Dip-tera: Culicidae) in Turkey. J Pestic Sci, 25; 34(4):259-64.
24. Chen L, Zhong D, Zhang D, et al (2010). Molecular ecology of pyrethroid knock-down resistance in Culex pipiens pallens mosquitoes. PLoS One, 5(7):e11681.
25. Liu HM, Cheng P, Huang X, et al (2013). Identification of TCT, a novel knock-down resistance allele mutation and anal-ysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China. Mol Med Rep, 7(2):525-30.
26. Xu Q, Liu H, Zhang L, et al (2005). Re-sistance in the mosquito, Culex quinquefas-ciatus, and possible mechanisms for re-sistance. Pest Manag Sic, 61(11):1096-102.
27. Wondji CS, Priyanka De Silva WA, Hem-ingway J, et al (2008). Characterization of knockdown resistance in DDT and pyre-throid resistant Culex quinquefasciatus popu-lations from Sri Lanka. Trop Med Int Health, 13(4):548-55.
28. Bkhache M, Tmimi FZ, Charafeddine O, et al (2016). First report of L1014F-kdr mu-tation in Culex pipiens complex from Mo-rocco. Parasit Vectors, 9(1):644.
29. Krypuy M, Newnham GM, Thomas DM, et al (2006) High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS co-don 12 and 13 mutations in non-small cell lung cancer. BMC Cancer, 6:295.
30. United States President’s Malaria Initiative. PMI guidelines for entomological monitoring and insecticide resistance management. Washington DC, 2010.
31. United States Agency for International De-velopment, United States President’s Ma-laria Initiative, RTI International. An eco-nomic analysis of the costs of indoor re-sidual spraying in 12 PMI countries 2008–2010. Draft, 2011.
32. Worrall, E, Conner SJ, Thomson MC (2008). Improving the cost effectiveness of IRS with climate informed health sur-veillance systems. Malar J, 7:263.
2. Sarkar M, Baruah I, Srivastava RB, et al (2011). High throughput approach to de-tection of knockdown resistance (kdr) mutation in mosquitoes, Culex quinquefasci-atus, based on real time PCR using single labeled hybridization probe/melting curve analysis. Pest Manag Sci, 67(2):156-61.
3. Hamer GL, Kitron UD, Brawn JD et al (2008). Culex pipiens (Diptera: Culicidae): a bridge vector of West Nile virus to hu-mans. J Med Entomol, 45 (1):125-8.
4. Burton MJ, Mellor IR, Duce IR, et al (2011). Differential resistance of insect sodium channels with kdr mutations to deltame-thrin, permethrin and DDT. Insect Biochem Mol Biol, 41(9):723-32.
5. Yanola J, Chamnanya S, Lumjuan N, et al (2015). Insecticides resistance in the Culex quinquefasciatus populations from northern Thailand and possible resistance mecha-nisms. Acta Trop,149:232-8.
6. Sarkar M, Borkotoki A, Baruah I, et al (2009). Molecular analysis of knock down resistance (kdr) mutation and distribution of kdr genotypes in a wild population of Culex quinquefasciatus from India. Trop Med Int Health, 14(9):1097-104.
7. WC Marquardt. The biology of disease vectors. Elsevier Academic Press, Burlington, Massachusetts. (1996): 595-605. 632 pp.
8. Hemingway J, Ranson H (2000). Insecticide resistance in insect vectors of human dis-ease. Annu Rev Entomol, 45:371-91.
9. Soderlund DM, Bloomquist JR (1989). Neu-rotoxic actions of pyrethroid insecticides. Annu Rev Entomol, 34:77-96.
10. Bass C, Nikou D, Donnelly MJ, et al (2007). Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a compari-son of two new high-throughput assays with existing methods. Malar J, 6:111.
11. Brengues C, Hawkes NJ, Chandre F, et al (2003). Pyrethroid and DDT cross‐resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated so-dium channel gene. Med Vet Entomol, 17(1):87-94.
12. Ranson H, Jensen B, Vulule JM, et al (2000). Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol Biol, 9(5):491-7.
13. Liew M, Pryor R, Palais R, et al (2004). Gen-otyping of single-nucleotide polymor-phisms by high-resolution melting of small amplicons. Clin Chem, 50(7):1156-64.
14. Do H, Krypuy M, Mitchell PL, et al (2008). High resolution melting analysis for rapid and sensitive EGFR and KRAS mutation detection in formalin fixed paraffin em-bedded biopsies. BMC Cancer, 8:142.
15. WHO (2014). Pesticide Evaluation Scheme. Discriminating concentrations of insecti-cide for adult mosquitoes. WHO Tech-nical Report Series 818. pp 40.
16. WHO (2016). Test procedures for insecticide re-sistance 318 monitoring in malaria vector mosqui-toes. 2nd edition. World Health Organiza-tion, Geneva. Pp 54.
17. Shayan P, Borji H, Eslami A, et al (2007). Isolation of DNA from a single Hel-minth using new developed Kit in Iran and its PCR analysis. Iran J Parasitol, 2(2):34-9.
18. Corbel V, N’guessan R, Brengues C, et al (2007). Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa. Acta Trop, 101(3):207-16.
19. Martinez Torres D, Chevillon C, Brun Bara-le A, et al (1999). Voltage-dependent Na+ channels in pyrethroid-resistant Culex pipiens L mosquitoes. Pestic Sci, 55(10):1012-20.
20. Fathian M, Vatandoost H, Moosa-Kazemi SH, et al (2014). Susceptibility of Culicidae mosquitoes to some insecticides recom-mended by WHO in a malaria endemic area of southeastern Iran. J Arthropod Borne Dis, 9(1):22-34.
21. NaseriKarimi N, Vatandoost H, Bagheri M, et al (2015). Susceptibility status of Culex pipiens against deltamethrin and DDT, Urmia County, West Azerbaijan Province, northwestern Iran. Asian Pac J Trop Dis, 5(Suppl 1): S77-S79.
22. Salim-Abadi Y, Oshaghi MA, Enayati AA et al (2016). High Insecticides Resistance in Culex pipiens (Diptera: Culicidae) from Tehran, Capital of Iran. Iran J Arthropod Borne Dis, 10(4):483-92.
23. Akiner MM, Simsek MF, Caglar SS (2009). Insecticide resistance of Culex pipiens (Dip-tera: Culicidae) in Turkey. J Pestic Sci, 25; 34(4):259-64.
24. Chen L, Zhong D, Zhang D, et al (2010). Molecular ecology of pyrethroid knock-down resistance in Culex pipiens pallens mosquitoes. PLoS One, 5(7):e11681.
25. Liu HM, Cheng P, Huang X, et al (2013). Identification of TCT, a novel knock-down resistance allele mutation and anal-ysis of resistance detection methods in the voltage-gated Na+ channel of Culex pipiens pallens from Shandong Province, China. Mol Med Rep, 7(2):525-30.
26. Xu Q, Liu H, Zhang L, et al (2005). Re-sistance in the mosquito, Culex quinquefas-ciatus, and possible mechanisms for re-sistance. Pest Manag Sic, 61(11):1096-102.
27. Wondji CS, Priyanka De Silva WA, Hem-ingway J, et al (2008). Characterization of knockdown resistance in DDT and pyre-throid resistant Culex quinquefasciatus popu-lations from Sri Lanka. Trop Med Int Health, 13(4):548-55.
28. Bkhache M, Tmimi FZ, Charafeddine O, et al (2016). First report of L1014F-kdr mu-tation in Culex pipiens complex from Mo-rocco. Parasit Vectors, 9(1):644.
29. Krypuy M, Newnham GM, Thomas DM, et al (2006) High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS co-don 12 and 13 mutations in non-small cell lung cancer. BMC Cancer, 6:295.
30. United States President’s Malaria Initiative. PMI guidelines for entomological monitoring and insecticide resistance management. Washington DC, 2010.
31. United States Agency for International De-velopment, United States President’s Ma-laria Initiative, RTI International. An eco-nomic analysis of the costs of indoor re-sidual spraying in 12 PMI countries 2008–2010. Draft, 2011.
32. Worrall, E, Conner SJ, Thomson MC (2008). Improving the cost effectiveness of IRS with climate informed health sur-veillance systems. Malar J, 7:263.
| Files | ||
| Issue | Vol 48 No 6 (2019) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v48i6.2916 | |
| Keywords | ||
| Culex pipiens Knockdown resistance Deltamethrin | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
ZEIDABADINEZHAD R, VATANDOOST H, ABAI MR, DINPARAST DJADID N, RAZ A, SEDAGHAT MM, OSHAGHI MA, RAEISI A, ADIBI N. Target Site Insensitivity Detection in Deltamethrin Resistant Culex pipiens Complex in Iran. Iran J Public Health. 2019;48(6):1091-1098.
