Original Article

Genetic and Phylogenetic Characterization of the M Gene of Influenza A Virus Isolated from Iranian Patients

Abstract

Background: A few studies have been done on the molecular analysis of Iranian influenza A isolates M gene.

Methods: In 2014, nasal swabs collected from outpatients with clinical symptoms in the hospital clinics of Tehran, Iran were subjected for influenza detection and subtyping using Real-Time RT-PCR. Sequence and phylogenetic analysis performed on four randomly selected isolates from each subtype (H1N1 and H3N2) using neighbor-joining method.

Results: Phylogenetic dendrograms drawn based on M nucleotide sequence of H1N1 isolates showed close relatedness with Omanian isolates while the most isolates of H3N2 have clustered with Kuwait isolates and isolates from outside of geographical location. Amino acid sequence analysis showed S31N substitution in all isolates rendering the virus resistant to adamantanes.

Conclusion: This study determined the sequence identity and phylogenetic relatedness of M gene sequence got from Iranian influenza A isolates to elucidate the modality of relationship of this gene in comparison with its counterparts from other regions.

 

1. Medina RA, García-Sastre A (2011). Influen-za A viruses: new research developments. Nat Rev Microbiol, 9(8): 590-603.
2. Li TC, Chan MC, Lee N (2015). Clinical im-plication of antiviral resistance in influen-za. Viruses, 7(9): 4929-44.
3. Treanor JJ (2014). Influenza Viruses. In: Vi-ral Infections of Humans- epidemiology and control. Ed, RA Kaslow, LR Stan-berry, JW Le Duc. Springer Sci-ence+Business Media, New York, USA, pp. 455-78.
4. Saito R, Li D, Shimomura C, Masaki H et al (2006). An off-seasonal amantadine-resistant H3N2 influenza outbreak in Ja-pan. Tohoku J Exp Med, 210(1): 21-7.
5. Pielak RM, Chou JJ (2011). Influen-za M2 proton channels. Biochim Biophys Acta, 1808(2): 522-9.
6. Król E, Rychłowska M, Szewczyk B (2014). Antivirals-current trends in fighting influenza. Acta Biochim Pol, 61(3): 495-504.
7. Yavarian J, Azad TM, Zheng X et al (2010). Amantadine resistance in relation to the evolution of influenza A (H3N2) viruses in Iran. Antiviral Res, 88(2): 193-6.
8. Ghaderi M, Mokhtari-Azad T, Shah-mahmoodi Sh et al (2010). Amantadine-resistant among seasonal H1N1 and 2009 pandemic isolated of influenza A vi-ruses in Iran. Iran J Virol, 4(1): 12-6.
9. World Health Organization (2009). CDC protocol of realtime RTPCR for influenza A (H1N1).
10. Acharya R, Carnevale V, Fiorin G et al (2010). Structure and mechanism of pro-ton transport through the transmem-brane tetrameric M2 protein bundle of the influenza A virus. Proc Natl Acad Sci USA, 107(34): 15075-80.
11. Furuse Y, Suzuki A, Kamigaki T, Oshitani H (2009). Evolution of the M gene of the influenza A virus in different host spe-cies: large-scale sequence analysis. Virol J, 6: 67.
12. Schnell JR, Chou JJ (2008). Structure and mechanism of the M2 proton channel of influenza A virus. Nature, 451(7178): 591-5.
13. Bright RA, Medina MJ, Xu X et al (2005). Incidence of adamantane resistance among influenza A (H3N2) viruses isolat-ed worldwide from 1994 to 2005: a cause for concern. Lancet, 366 (9492): 1175-81.
14. Dong G, Peng C, Luo J et al (2015). Ada-mantane-resistant influenza A viruses in the world (1902-2013): frequency and dis-tribution of M2 gene mutations. PLoS One, 10(3): e0119115.
15. Hayden FG, de Jong MD (2011). Emerging influenza antiviral resistance threats. J In-fect Dis, 203(1): 6-10.
16. Nelson MI, Simonsen L, Viboud C et al (2009). The origin and global emergence of adamantine resistant A (H3N2) influ-enza viruses. Virology, 388(2): 270-8.
17. Abed Y, Goyette N, Boivin G (2005). Gen-eration and characterization of
recombinant influenza A(H1N1) viruses harboring amantadine resistance muta-tions. Antimicrob Agents Chemother, 49(2): 556-9.
18. Deyde VM, Xu X, Bright RA et al (2007). Surveillance of resistance to adamantanes among influenza A (H3N2) and A (H1N1) viruses isolated worldwide. J Infect Dis, 196(2): 249-57.
19. Hurt AC (2014). The epidemiology and spread of drug resistant human influenza viruses. Curr Opin Virol, 8: 22-9.
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IssueVol 48 No 3 (2019) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijph.v48i3.897
Keywords
Influenza A virus Iran M gene Phylogenetic analysis Real-time reverse transcription PCR (RT-PCR)
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How to Cite
1.
BEHZADIAN F, MOASSER E, OWLIA P, SADERI H. Genetic and Phylogenetic Characterization of the M Gene of Influenza A Virus Isolated from Iranian Patients. Iran J Public Health. 2019;48(3):525-530.