Original Article

Candiduria in Hospitalized Patients and Identification of Isolated Candida Species by Morphological and Molecular Methods in Ilam, Iran


Background: Candiduria in hospitalized patients may represent contamination, colonization, or urinary tract infections. On the other hand, candidemia and upper urinary tract infection could be the complications of candiduria. The aim of this study was to determine candiduria in hospitalized patients and identify isolated Candida species by conventional and molecular methods.

Methods: This cross-sectional study was conducted on hospitalized patients in Imam Khomeini and Mostafa Khomeini hospitals in Ilam, western Iran from Jan to Dec 2016. Urine samples of hospitalized patients were collected during a period of 4 months for diagnosis of candiduria. Primary identification was done by conventional methods. PCR profile was carried out using phenol-chloroform method and confirmed using restriction fragment length polymorphism (PCR-RFLP) technique by MspI restriction enzyme.

Results: Candiduria was diagnosed in 18 (9.2%) cases from a total of 195 patients. Isolated yeasts were identified as C. albicans (n: 13), C. glabrata (n: 5), and C. parapsilosis (n: 1) in the one case both C. albicans and C. glabrata were isolated from a urine sample.

Conclusion: Candida urinary tract infection is becoming increasingly common in hospitalized patients but, differentiation fungal colonization from infection and identification of etiologic agents for optimal treatment is necessary.


1. Krcmery S, Dubrava M, Krcmery V Jr (1999). Fungal urinary tract infections in patients at risk. Int J Antimicrob Agents, 11(3-4): 289-291.
2. Rathor N, Khillian V, Arvind Kumar Baro-nia (2014). Nasocomial candiduria in chronic liver disease patients at a hepato-biliary center. Indian J Crit Care Med, 18(9): 639–640
3. Fisher JF, Kavanage K, Sobel JD (2014).Candida urinary tract infection: pathogenesis. Clin Infect Dis, 52 Suppl 6:S437-51.
4. Khan A, El-Charabaty E, El-Sayegh S (2015). Fungal Infections in Renal Trans-plant Patients. J Clin Med Res, 7(6): 371-378.
5. Jacqueline M Achkar, Bettina C (2010). Can-dida infections of the genitourinary tract. Clin Microbiol Rev, 23(2):253-273.
6. Badiee P, Alborzi A (2011). Invasive fungal infections in renal transplant recipients. Exp Clin Transplant, 9(6):355-62.
7. Yashavanth, R, Shiju MP, Bhaskar UA et al (2013). Candiduria: Prevalence and Trends in Antifungal Susceptibility in a Tertiary Care Hospital of Mangalore. J Clin Diagn Res, 7(11): 2459-2461.
8. Lundstrom T1, Sobel J (2001). Nosocomial candiduria: a review. Clin Infect Dis, 32(11):1602-7.
9. Yaghoubi R, Kachuei R, Kordbacheh P, Re-zai S (2016). Candiduria in renal trans-plant recipients and identification of iso-lated candida species by morphological and molecular methods. Adv Biores, 7 (4): 180-185
10. Zarei-Mahmoudabadi A, Zarrin M, Ghanatir F et al (2012). Candiduria in hospitalized patients in teaching hospital of Ahvaz. Iran J Microbiol, 4(4):198-203.
11. Ghahri M, Hosseini S, Davarzani R et al (2013). Identification of isolated yeasts from urinary tract in patients with urinary catheter by PCR-RFLP. J Lab Diagn, 22: 53-60.
12. Ricea J, Safdarb N, et al (2009). Urinary Tract Infections in Solid Organ Trans-plant Recipients. Am J Transplant, 9 Suppl 4:S267-72.
13. Safdar N, Slattery W R, Knasinski V et al (2005). Predictors and Outcomes of Candiduria in Renal Transplant Recipi-ents. Clin Infect Dis, 40: 1413-1421.
14. Nademi A, Shahrokh H, Kordbacheh P, et al (2015). Identification and antifungal susceptibility pattern of Candida species isolated from patients with nasocomial candiduria. J Mycol Res, 2(2):77-84.
15. Delgado J, Calvo N, Gomis A et al (2010). Candiduria in Renal Transplant Recipi-ents: Incidence, Clinical Repercussion, and Treatment Indication. Transplant Proc, 42(8):2944-6.
16. Febré N, Silva V, Medeiros EA et al (1999). Microbiological Characteristics of Yeasts Isolated from Urinary Tracts of Intensive Care Unit Patients Undergoing Urinary Catheterization. J Clin Microbiol, 37(5): 1584–1586.
17. Bakhary AZ (2008). Candiduria: A review of clinical significance and manaegement. Saudi J Kidney Dis Transplant, 19(3): 350-360.
18. Calderone, RA (2002). Candida and candidi-asis. Emerg Infect Dis, 8(8): 876.
19. Rezai MS, Vaezi A, Fakhim H et al (2017). Successful treatment with caspofungin of candiduria in a child with Wilms tumor; review of literature. J Mycol Med, 27(2):261-265.
20. Zaini F, Azordegan F, Chabavizadeh J (1993). Study of fungal infections in urine. Iran J Public Health, 22: 13-31.
21. Fakour F, Falahati M, Zaini F, Mousavi Nasab N (2004). A Survey of Candiduria in Diabetic Patients of Zanjan, 2001-2002. Razi J Med Sci, 11(41): 453-462.
IssueVol 48 No 1 (2019) QRcode
SectionOriginal Article(s)
DOI https://doi.org/10.18502/ijph.v48i1.804
Hospitalized patients Candiduria Candida albicans Candida spp Yeast

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
FAZELI A, KORDBACHEH P, NAZARI A, DAIE GHAZVINI R, MIRHENDI H, SAFARA M, BAKHSHI H, YAGHOUBI R. Candiduria in Hospitalized Patients and Identification of Isolated Candida Species by Morphological and Molecular Methods in Ilam, Iran. Iran J Public Health. 2019;48(1):156-161.