Original Article

Adiponectin Participates in Preeclampsia by Regulating the Biological Function of Placental Trophoblasts through P38 MAPK-STAT5 Pathway

Abstract

Background: We aimed to investigate the participation of adiponectin in preeclampsia, and to explore the possible mechanism.

Methods: A total of 52 patients with preeclampsia and 30 normal women with full-term pregnancy were enrolled. Immunohistochemistry was used to detect the localization of MAPK and STAT5. RT-PCR was used to detect the expression of adiponectin mRNA in placental tissue of patients with preeclampsia and normal pregnant women. Western blot was used to detect the expression of adiponectin protein, MAPK, p-MAPK, STAT5 and p-STAT5 in placental tissue of patients with preeclampsia and normal pregnant women.

Results: p-p38 was highly expressed in placental trophoblasts of patients with preeclampsia, while p-STAT5 was less expressed. Expression level of p-p38 and p-STAT5 in patients with preeclampsia were significantly different from those in normal pregnant women (P<0.01). Expression level of adiponectin mRNA was significantly lower in patients with preeclampsia than in normal pregnant women (P<0.05). Level of p-p38 expression was negatively correlated with levels of adiponectin expression (r=-0.413, P<0.05). Expression level of p-STAT5 was positively correlated with expression level of adiponectin (r=0.526, P<0.01).

Conclusion: Adiponectin participates in preeclampsia by regulating the biological function of placental trophoblasts through p38 MAPK-STAT5 pathway.

 

1. Wang T, Zhou R, Gao L et al (2014). Elevation of urinary adipsin in preeclampsia: correlation with urine protein concentration and the po-tential use for a rapid diagnostic test. Hyperten-sion, 64: 846-851.
2. Martinez-Fierro ML, Garza-Veloz I, Carrillo-Sanchez K et al (2014). Expression levels of seven candidate genes in human peripheral blood mononuclear cells and their association with preeclampsia. Hypertens Pregnancy, 33: 191-203.
3. Khosrowbeygi A, Ahmadvand H (2013). Leptin to adiponectin ratio in preeclampsia. Bangla-desh Med Res Counc Bull, 39: 18-21.
4. Machado JS, Cavalli RC, Sandrim VC et al(2012). PP002. Study of polymorphisms of the adiponectin gene in women with gesta-tional hypertension and preeclampsia. Pregnan-cy Hypertens, 2(3): 241.
5. Eleuterio NM, Palei AC, Rangel Machado JS, Tanus-Santos JE, Cavalli RC and Sandrim VC (2015). Positive correlations between circulat-ing adiponectin and MMP2 in preeclampsia pregnant. Pregnancy Hypertens, 5: 205-208.
6. Schoen CN, Moreno SC, Saccone G et al (2018). Outpatient versus inpatient management for superimposed preeclampsia without severe features: a retrospective, multicenter study. J Matern Fetal Neonatal Med 31(15):1993-1999.
7. Salihu HM, De La Cruz C, Rahman S and Au-gust EM (2012). Does maternal obesity cause preeclampsia? A systematic review of the evi-dence. Minerva Ginecol, 64: 259-280.
8. Song Y, Gao J, Qu Y, Wang S, Wang X, Liu J (2016). Serum levels of leptin, adiponectin and resistin in relation to clinical characteris-tics in normal pregnancy and preeclampsia. Clin Chim Acta, 458: 133-137.
9. Demir BC, Atalay MA, Ozerkan K, Doster Y, Ocakoglu G and Kucukkomurcu S (2013). Maternal adiponectin and visfatin concentra-tions in normal and complicated pregnancies. Clin Exp Obstet Gynecol, 40: 261-267.
10. Eleuterio NM, Palei AC, Rangel Machado JS, Tanus-Santos JE, Cavalli RC and Sandrim VC (2014). Correlations between circulating levels of adipokines and anti-angiogenic factors in women with BMI <30 and a late-onset preeclampsia. Hypertens Pregnancy, 33: 72-80.
11. Machado JS, Palei AC, Amaral LM et al (2014). Polymorphisms of the adiponectin gene in gestational hypertension and pre-eclampsia. J Hum Hypertens, 28: 128-132.
12. Huda SS, Jordan F, Bray J, Love G, Payne R, Sattar N, Freeman DJ (2017). Visceral adi-pose tissue activated macrophage content and inflammatory adipokine secretion is higher in pre-eclampsia than in healthy pregnancys. Clin Sci (Lond), 131: 1529-1540.
13. Khosrowbeygi A, Ahmadvand H (2013). Posi-tive correlation between serum levels of adi-ponectin and homocysteine in pre-eclampsia. J Obstet Gynaecol Res, 39: 641-646.
14. Drost JT, Maas AH, Holewijn S et al (2014). Novel cardiovascular biomarkers in women with a history of early preeclampsia. Atherosclerosis, 237: 117-122.
15. Lindley KJ, Conner SN, Cahill AG, Novak E, Mann DL (2017). Impact of Preeclampsia on Clinical and Functional Outcomes in Women With Peripartum Cardiomyopathy. Circ Heart Fail, 10: e003797.
16. Buke B, Topcu HO, Engin-Ustun Y, Danisman N (2014). Comparison of serum maternal adiponectin concentrations in women with iso-lated intrauterine growth retardation and intrauterine growth retardation concomitant with pre-eclampsia. J Turk Ger Gynecol Assoc, 15: 173-176.
17. Garces MF, Peralta JJ, Ruiz-Linares CE et al (2014). Irisin levels during pregnancy and changes associated with the development of preeclampsia. J Clin Endocrinol Metab, 99: 2113-2119.
18. Tobinaga CM, Torloni MR, Gueuvoghlanian-Silva BY, Pendeloski KP, Akita PA, Sass N, Daher S (2014). Angiogenic factors and uter-ine Doppler velocimetry in early- and late-onset preeclampsia. Acta Obstet Gynecol Scand, 93: 469-476.
19. Eleuterio NM, Palei AC, Rangel Machado JS, Tanus-Santos JE, Cavalli RC, Sandrim VC (2013). Relationship between adiponectin and nitrite in healthy and preeclampsia pregnan-cies. Clin Chim Acta, 423: 112-115.
20. Salimi S, Farajian-Mashhadi F, Naghavi A, Mokhtari M, Shahrakipour M, Saravani M, Yaghmaei M (2014). Different profile of se-rum leptin between early onset and late onset preeclampsia. Dis Markers, 2014: 628476.
21. Foidart JM, Schaaps JP, Chantraine F, Munaut C, Lorquet S (2009). Dysregulation of anti-angiogenic agents (sFlt-1, PLGF, and sEn-doglin) in preeclampsia--a step forward but not the definitive answer. J Reprod Immunol, 82:106-111.
22. Tannetta DS, Dragovic RA, Gardiner C, Red-man CW, Sargent IL (2013). Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin. PLoS One, 8:e56754.
23. Gangooly S, Muttukrishna S, Jauniaux E (2014). In-vitro study of the effect of anti-hypertensive drugs on placental hormones and angiogenic proteins synthesis in pre-eclampsia. PLoS One, 9:e107644.
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IssueVol 47 No 12 (2018) QRcode
SectionOriginal Article(s)
Keywords
Adiponectin MAPK STAT5 Preeclampsia
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How to Cite
1.
DONG G, TIAN Y, LI X. Adiponectin Participates in Preeclampsia by Regulating the Biological Function of Placental Trophoblasts through P38 MAPK-STAT5 Pathway. Iran J Public Health. 2018;47(12):1838-1844.