Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echi-nococcus granulosus Sensu Stricto Complex

  • Abolghasem SIYADATPANAH 1. Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran 2. Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
  • Ahmad DARYANI Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Shahabeddin SARVI Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Adel SPOTIN Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mehdi SHARIF Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Reza ESMAEELZADEH DIZAJI Department of Poultry Disease, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Davood Anvari Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Amir EMAMI ZEYDI Department of Medical-Surgical Nursing, Nasibeh School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran
  • Mohammad Hasan KOHANSAL School of Medicine, Bam University of Medical Sciences, Bam, Iran
  • Nelson Ivan AGUDELO HIGUITA Division of Infectious Diseases Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
  • Seyed Abdollah HOSSEINI Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Mojgan ARYAEIPOUR Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Shirzad GHOLAMI Mail Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
Keywords:
Human hydatidosis, Genetic diversity, Phylogeography, Iran

Abstract

Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics.

Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity.

Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified.

Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.

Author Biographies

Amir EMAMI ZEYDI, Department of Medical-Surgical Nursing, Nasibeh School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran

 

 

Mohammad Hasan KOHANSAL, School of Medicine, Bam University of Medical Sciences, Bam, Iran

 

 

Seyed Abdollah HOSSEINI, Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran

 

 

Mojgan ARYAEIPOUR, Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

 

 

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Published
2020-08-24
How to Cite
1.
SIYADATPANAH A, DARYANI A, SARVI S, SPOTIN A, SHARIF M, ESMAEELZADEH DIZAJI R, Anvari D, EMAMI ZEYDI A, KOHANSAL MH, AGUDELO HIGUITA NI, HOSSEINI SA, ARYAEIPOUR M, GHOLAMI S. Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echi-nococcus granulosus Sensu Stricto Complex. Iran J Public Health. 49(9):1758-1768.
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Original Article(s)