The Frequency of Resistance Genes in Salmonella enteritidis Strains Isolated from Cattle
Background: Salmonella enteritidis causes infections in humans and animals. Antibiotics are used to eliminate bacterial infections, which become resistant to antibiotics after a while. This study aimed to isolate S. enteritidis from cattle feces samples and also to evaluate the frequency of genes associated with multi-drug resistance (MDR).
Methods: One hundred ten fecal samples of cattle were collected from Jul to Dec, 2017 in Khuzestan Province, southern Iran. Bacterial culture and molecular methods were used to isolate and identify S. enteritidis. Disk diffusion method was used to assess antibiotic susceptibility. Then Polymerase chain reaction (PCR), assay was used for definitive diagnosis of S. enteritidis and resistance genes.
Results: Overall, 101 (91.81%) samples were detected to be contaminated with Salmonella genus and 86 samples (85.14%) were identified as S. enteritidis. The highest and lowest antibiotic resistance were belonged to gentamicin (n=70, 81.39%), and tetracycline (n=6, 6.97%). Besides, 64 samples (74.42%) had 2-10 drugs resistance patterns. Moreover, the highest and the least resistance were related to blaIMP-1 (n=73, 84.88%) and tet(B) (n=49, 56.97%) genes respectively.
Conclusion: The drug-induced genes in S. enteritidis have a high frequency. Therefore, antibiotic resistance and high MDR to antibiotics can be due to the incorrect use of antibiotics and the lack of health monitoring in Cattle farms.
2. Ranjbar R, Naghoni A, Afshar D et al (2016). Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera. Osong Public Health Res Perspect, 7(6):373-377.
3. Doosti A, Mahmoudi E, Jami MS et al (2016). Prevalence of aadA1, aadA2, aadB, strA and strB genes and their associations with multidrug resistance phenotype in Salmonella Typhimurium isolated from poultry carcasses. Thai J Vet Med, 46 (4):691-697.
4. Crum Cianflone N F (2008). Salmonellosis and the GI Tract: More than Just Peanut Butter. Curr Gastroenterol Rep, 10(4):424-431.
5. Bula-Rudas FJ, Rathore MH, Maraqa NF (2015). Salmonella Infections in Child-hood. Adv Pediatr, 62(1):29-58.
6. Naghoni A, Ranjbar R, Tabaraie B et al (2010). High prevalence of integron-mediated resistance in clinical isolates of Salmonella enterica. Jpn J Infect Dis, 63(6):417-421.
7. Eng SK, Pusparajah P, Mutalib NSA et al (2015). Salmonella: A review on patho-genesis, epidemiology and antibiotic re-sistance. Frontiers in Life Science, 8(3):284-293.
8. Chen HM, Wang Y, Su LH et al (2013). Nontyphoid Salmonella infection: micro-biology, clinical features, and antimicrobi-al therapy. Pediatr Neonatol, 54(3):147-152.
9. Alekshun MN, Levy SB (2007). Molecular mechanisms of antibacterial multidrug re-sistance. Cell, 128(6):1037-1050.
10. Ranjbar R, Giammanco GM, Aleo A et al (2010). Characterization of the first ex-tended-spectrum beta-lactamase-producing nontyphoidal Salmonella strains isolated in Tehran, Iran. Foodborne Pathog Dis, 7(1):91-95.
11. Ranjbar R, Naghoni A, Yousefi S et al (2013). The Study of Genetic Relationship Among Third Generation Cephalospor-in-resistant Salmonella enterica Strains by ERIC-PCR. Open Microbiol J, 7:142-145.
12. Ma M, Wang H, Yu Y et al (2007). Detection of antimicrobial resistance genes of path-ogenic Salmonella from swine with DNA microarray. J Vet Diagn Invest, 19(2):161-167.
13. Chen S, Zhao S, White DG et al (2004). Characterization of multiple-antimicrobial-resistant Salmonella serovars isolated from retail meats. Appl Environ Microbiol, 70(1):1-7.
14. Adesiji YO, Deekshit VK, Karunasagar I (2014). Antimicrobial-resistant genes as-sociated with Salmonella spp. isolated from human, poultry, and seafood sources. Food Sci Nutr, 2(4):436-442.
15. Doosti A, Doosti E, Rahimi E et al (2017). Frequency of Antimicrobial-Resistant Genes in Salmonella enteritidis Isolated from Traditional and Industrial Iranian White Cheeses. Proc Natl Acad Sci India Sect B Biol Sci, 87(1):73-80.
16. Afshari A, Baratpour A, Khanzade S et al (2018). Salmonella Enteritidis and Salmo-nella Typhimorium identification in poul-try carcasses. Iran J Microbiol, 10(1):45-50.
17. Bonardi S, Bruini I, Magnani R et al (2017). Low Prevalence of Salmonella Enterica in Cull Dairy Cattle at Slaughter in Northern Italy. Ital J Food Saf, 6(1):6172.
18. Ghaderi R, Moradi Bidhendi S, Khaki P (2016). Occurrence of multidrug-resistant Salmonella enterica serovar Enteritidis iso-lates from poultry in Iran. ARI, 71(1):43-49.
19. Ahmed AM, Shimamoto T, Shimamoto T (2014). Characterization of integrons and resistance genes in multidrug-resistant Salmonella enterica isolated from meat and dairy products in Egypt. Int J Food Micro-biol, 189:39-44.
20. Randall LP, Cooles SW, Osborn MK et al (2004). Antibiotic resistance genes, in-tegrons and multiple antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK. J Antimicrob Chemother, 53(2):208-216.
21. Jahandeh N, Ranjbar R, Behzadi P et al (2015). Uropathogenic Escherichia coli viru-lence genes: invaluable approaches for designing DNA microarray probes. Cent European J Urol, 68(4):452-458
22. Ranjbar R, Haghi-Ashtiani M, Jonaidi Jafari N et al (2009). The Prevalence and Anti-microbial Susceptibility of Bacterial Uro-pathogens Isolated from Pediatric Pa-tients. Iran J Public Health, 38(2):134-138.
23. Kemal J, Sibhat B, Menkir S et al (2015). An-timicrobial resistance patterns of Salmo-nella in Ethiopia: A review. Afr J Microbiol Res, 9(46):2249-2256.
24. Firoozeh F, Zahraei-Salehi T, Shahcheraghi F et al (2012). Characterization of class I integrons among Salmonella enterica serovar Enteritidis isolated from humans and poultry. FEMS Immunol Med Microbiol, 64(2):237-243.
|Issue||Vol 49 No 5 (2020)|
|Salmonella enteritidis Resistance gene; Multi-drug resistance; Cattle|
|Rights and permissions|
|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|