Original Article

Application Values of T-SPOT.TB in Clinical Rapid Diagnosis of Tuberculosis



Background: This paper aims to explore the application value of tuberculosis-specific enzyme-linked immunospot assay (T-SPOT.TB) in the diagnosis of tuberculosis.

Methods: Fifty one patients with tuberculosis (TB) admitted to Wuxi No.5 People’s Hospital, Wuxi, China from June 2015 to June 2017 were selected as the TB group, and 40 patients without tuberculosis admitted in the same period were randomly selected as the non-TB group. Patients in the two groups received T-SPOT.TB, TB antibody (TB-Ab) test and mycobacterium TB deoxyribonucleic acid (TB-DNA) test, and the results were compared.

Results: Comparisons of the sensitivity of the three methods showed that the sensitivity of T-SPOT.TB was the highest, followed by TB-DNA from sputum samples, and that of TB-Ab was the lowest. The specificity of TB-Ab was the highest, followed by T-SPOT.TB, and that of TB-DNA from sputum samples was the lowest. In the receiver operating characteristic (ROC) curve analysis, the area under curve (AUC) of T-SPOT.TB (0.896) was the highest, followed by TB-DNA from sputum samples (0.772), and that of sputum smears (0.698) was the lowest.

Conclusion: T-SPOT.TB can quickly and accurately determine the presence of tuberculosis infection, and it is a non-invasive examination, which can further assist in the diagnosis and guide the treatment.




Khaliq A, Batool SA, Chaudhry MN (2015). Seasonality and trend analysis of tubercu-losis in Lahore, Pakistan from 2006 to 2013. J Epidemiol Glob Health, 5: 397-403.

Organization WH (2016). Global Tuberculo-sis report 2016. Geneva: WHO, 15-42.

Lee YM, Park KH, Kim SM, Park SJ, Lee SO, Choi SH, Kim YS, Woo JH, Kim SH (2013). Risk factors for false-negative re-sults of T-SPOT.TB and tuberculin skin test in extrapulmonary tuberculosis. Infec-tion, 41: 1089-1095.

Ling DI, Nicol MP, Pai M, Pienaar S, Den-dukuri N, Zar HJ (2013). Incremental val-ue of T-SPOT.TB for diagnosis of active pulmonary tuberculosis in children in a high-burden setting: A multivariable anal-ysis. Thorax, 68: 860-866.

Chiappini E, Della BC, Bonsignori F, Sollai S, Amedei A, Galli L, Niccolai E, Del PG, Singh M, D'Elios MM, de Martino M (2012). Potential role of M. Tuberculosis specific IFN-gamma and IL-2 ELISPOT assays in discriminating children with ac-tive or latent tuberculosis. Plos One, 7: e46041.

Singh A, Dey AB, Mohan A, Sharma PK, Mitra DK (2012). Foxp3+ regulatory T cells among tuberculosis patients: Impact on prognosis and restoration of antigen specific IFN-gamma pro-ducing T cells. Plos One, 7: e44728.

Cho OH, Park KH, Park SJ et al (2011). Rapid diagnosis of tuberculous peritonitis by T cell-based assays on peripheral blood and peritoneal fluid mononuclear cells. J Infect, 62: 462-471.

Wang X, Tang J, Wang R, Chen C, Tan S, Yu F, Tao Y, Li Y (2016). Sputum endothelin-1 level is associated with active pulmonary tuberculosis and effectiveness of anti-tuberculosis chemotherapy. Exp Ther Med, 11: 1104-1108.

Fei B, Wu Z, Min K, Zhang J, Ding C, Wu H (2014). Interferon-gamma release assay in the di-agnosis of laryngeal tuberculosis. Acta Otolaryngol, 134: 314-317.

Chu HQ, Li B, Zhao L, Huang DD, Zhang ZM, Xu JF, Zhang JB, Gui T, Xu LY, Sun XW (2015). Chest imaging comparison between non-tuberculous and tuberculo-sis mycobacteria in sputum acid fast bacilli smear-positive patients. Eur Rev Med Pharmacol Sci, 19: 2429-2439.

Turtle L, Kemp T, Davies GR, Squire SB, Beeching NJ, Beadsworth MB (2012). In routine UK hospital practice T-SPOT.TB is useful in some patients with a modest pre-test probability of active tuberculosis. Eur J Intern Med, 23: 363-367.

Aiken AM, Hill PC, Fox A, McAdam KP, Jackson-Sillah D, Lugos MD, Donkor SA, Adegbola RA, Brookes RH (2006). Reversion of the ELISPOT test after treatment in Gambian tuberculo-sis cases. Bmc Infect Dis, 6: 66.

Bobadilla K, Sada E, Jaime ME et al (2013). Human phagosome processing of Myco-bacterium tuberculosis antigens is modu-lated by interferon-gamma and interleu-kin-10. Immunology, 138: 34-46.

Millington KA, Innes JA, Hackforth S et al (2007). Dynamic relationship between IFN-gamma and IL-2 profile of Myco-bacterium tuberculosis-specific T cells and antigen load. J Immunol, 178: 5217-5226.

Chung WK, Zheng ZL, Sung JY, Kim S, Lee HH, Choi SJ, Yang J (2010). Validity of interferon-gamma-release assays for the diagnosis of latent tuberculosis in hae-modialysis patients. Clin Microbiol Infect, 16: 960-965.

Richeldi L, Losi M, D'Amico R et al (2009). Performance of tests for latent tuber-culosis in different groups of immuno-compromised patients. Chest, 136: 198-204.

Marques CD, Duarte AL, de Lorena VM, Souza JR, Souza WV, Gomes YM, de Carvalho EM (2009). Evaluation of an interferon gamma assay in the diagnosis of latent tuberculosis infection in pa-tients with rheumatoid arthritis. Rheumatol Int, 30: 57-62.

Wang JL, Zhao GW, Zhang ZQ, Wang XF, Wang MS (2015). Clinicopathologic char-acteristics of pe-diatric tuberculous pleural effusion: A retrospective analysis of 112 consecutive cases. Eur Rev Med Pharmacol Sci, 19: 2978-2982.

Bathoorn E, Limburg A, Bouwman JJ, Bossink AW, Thijsen SF (2011). Diagnos-tic potential of an en-zyme-linked im-munospot assay in tuberculous pericardi-tis. Clin Vaccine Immunol, 18: 874-877.

Ling DI, Pai M, Davids V et al (2011). Are interferon-gamma release assays useful for diagnosing active tuberculosis in a high-burden setting? Eur Respir J, 38: 649-656.

IssueVol 47 No 1 (2018) QRcode
SectionOriginal Article(s)
T-SPOT.TB Anti-TB-Ab Mycobacterium TB-DNA TB

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How to Cite
ZHU F, OU Q, ZHENG J. Application Values of T-SPOT.TB in Clinical Rapid Diagnosis of Tuberculosis. Iran J Public Health. 2017;47(1):18-23.