Hepatic Proteins and Inflammatory Markers in Rheumatoid Arthritis Patients
-
Manel BEN-HADJ-MOHAMED
,
Souhir KHELIL
,
Mokhles BEN DBIBIS
,
Latifa KHLIFI
,
Hend CHAHED
,
Salima FERCHICHI
,
Elyes BOUAJINA
,
Abdelhédi MILED
Abstract
Background: Rheumatoid arthritis is an autoimmune inflammatory rheumatic disease that causes chronic synovial inflammation eventually leading to joint destruction and disability. The aim of this study was to determine the variations of hepatic proteins, myeloperoxidase, and iron in rheumatoid arthritis Tunisian patients and their implications in inflammation and in iron metabolism.
Methods: Overall, 172 patients from the Rheumatology Department of the University Hospital “Farhat Hached”, Sousse-Tunisia between 2011 and 2012, with rheumatoid arthritis (97.1% women, average age: 48±13 yr) and 147 healthy volunteers (70.1% women, average age: 46± 7 yr) were included in this study. Serum hepatic proteins (high-sensitive C-reactive protein, ceruloplasmin, albumin, transferrin, α-1-acid glycoprotein and haptoglobin) were assessed by immunoturbidimetry (COBAS INTEGRA 400, Roche) and ferritin was measured by a microparticulate immunoenzymatic technic (AxSYM, ABBOTT, Germany), Plasma myeloperoxidase was determined by Enzyme-Linked Immunosorbent Assay. Serum iron was measured according to a colorimetric method at 595 nm (CX9-BECKMANN Coulter-Fuller-Ton, CA).
Results: Significantly higher levels of high-sensitive C-reactive protein, α-1-acid glycoprotein, Haptoglobin and myeloperoxidase in patients compared to controls (P<10-3). Albumin and iron rates were significantly decreased in patients compared to healthy group (P=0.026 and P<10-3, respectively). There were no differences between cases and controls for levels of ceruloplasmin, transferrin and ferritin (P=0.782, P=0.808, and P=0.175, respectively).
Conclusion: The high-sensitive C-reactive protein, α-1-acid glycoprotein, and haptoglobin increased in acute phase proteins in rheumatoid arthritis disease. The pro-inflammatory cytokines affect iron metabolism leading to the iron deficiency and rheumatoid anemia, which influenced Tf and ferritin levels.
Kurkó J, Besenyei T, Laki J, et al (2013). Genetics of rheumatoid arthritis - a comprehensive review. Clin Rev Allergy Immunol, 45 (2): 170-9.
Ruiz-Esquide V, Sanmartí R (2012). Tobacco and other environmental risk factors in rheumatoid arthritis. Reumatol Clin, 8 (6): 342-50.
Firestein GS (2003). Evolving concepts of rheumatoid arthritis. Nature, 423 (6937): 356-61.
Pattison D, Winyard PG (2008). Dietary antioxidants in inflammatory arthritis: do they have any role in etiology or therapy? Nat Clin Pract Rheumatol, 4 (11): 590–6.
Barber CE, Smith A, Esdaile JM, et al (2015). Best practices for cardiovascular disease prevention in rheumatoid arthritis: a systematic review of guideline recommendations and quality indicators. Arthritis Care Res (Hoboken), 67 (2): 169–79.
Edwards SW, Hallett MB (1997). Seeing the wood for the trees: the forgotten role of neutrophils in rheumatoid arthritis. Immunol Today, 18 (7): 320-4.
Mirshafiey A, MohsenzadeganM (2008). The role of reactive oxygen species in immunopathogenesis of rheumatoid arthritis. Iran J Allergy Asthma Immunol, 7 (4): 195–202.
Wright HL, Moots RJ, Bucknall RC, Edwards SW (2010). Neutrophil function in inflammation and inflammatory diseases. Rheumatology (Oxford), 49 (9): 1618-31.
Kettle AJ, Winterbourn CC (1997). Myeloperoxidase: a key regulator of neutrophil oxidant production. Redox Rep, 3(1): 3-15.
Saeki T, Kuroda T, Morita T, et al (1995). Significance of myeloperoxidase in rapidly progressive glomerulonephritis. Am J Kidney Dis, 26 (1): 13-21.
Stamp LK, Khalilova I, Tarr JM, et al (2012). Myeloperoxidase and oxidative stress in rheumatoid arthritis. Rheumatology (Oxford), 51 (10): 1796-803.
Segelmark M, Persson B, Hellmark T, Wieslander J (1997). Binding and inhibition of myeloperoxidase: a major function of ceruloplasmin? Clin Exp Immunol, 108 (1): 167-74.
Tapiero H, Townsend DM, Tew KD (2003). Trace elements in human physiology and pathology. Copper. Biomed Pharmacother, 57 (9): 386–98.
Kang JH, Kim SK, Choi SY, et al (2001). Oxidative modifi cation of human ceruloplasmin by peroxyl radicals. Biochem Biophys Acta, 1568 (1): 30–6.
Fox PL, Mukhopadyay C, Ehrenwald E (1995). Structure, oxidant activity, and cardiovascular mechanisms of human ceruloplasmin. Life Sci, 56 (21): 1749-58.
Masson C (2011). Rheumatoid anemia. Joint Bone Spine, 78 (2): 131–7.
Arnett FC, Edworthy SM, Bloch DA, et al (1988). The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum, 31 (3): 315-24.
Sarı RA, Taysi S, Erdem F, et al (2002). Correlation of serum levels of soluble intercellular adhesion molecule-1 with disease activity in systemic lupus erythematosus. Rheumatol Int, 21 (4): 149-52.
Black S, Kushner I, Samols D (2004). C-reactive protein. J Biol Chem, 279 (47): 48487-90.
Roberts WL; CDC/AHA (2004). CDC/AHA Workshop on Markers of Inflammation and Cardiovascular Disease: Application to Clinical and Public Health Practice: laboratory tests available to assess inflammation--performance and standardization: a back-ground paper. Circulation,110(25): e572-6.
Raynes JG, Eagling S, McAdam KP (1991). Acute-phase protein synthesis in human hepatoma cells: Differential regulation of serum amyloid A (SAA) and haptoglobin by interleukin-1 and interleukin-6. Clin Exp Immunol, 83 (3): 488-91.
Strecker D, Mierzecki A, Radomska K (2013). Copper levels in patients with rheumatoid Arthritis. Ann Agric and Environ Med, 20 (2): 312–6.
Cogalgil S, Taysi S (2002). Levels of antioxidant proteins and soluble intercellular adhesion molecule-1 in serum of patients with rheumatoid arthritis. Ann Clin Lab Sci, 32 (3): 264-70.
Louro MO, Cocho JA, Mera A, Tutor JC (2000). Immunochemical and enzymatic study of ceruloplasmin in rheumatoid arthritis. J Trace Elem Med Biol, 14 (3): 174-8.
Ryden L, Eaker D (1983). Identification of the thiol groups in human ceruloplasmin. Eur J Biochem, 132 (2): 241–7.
Klebanoff SJ (1992). Bactericidal effect of Fe2+, ceruloplasmin, and phosphate. Arch Biochem Biophys, 295 (2): 302–8.
Frieden E (1986). Perspectives on copper biochemistry. Clin Physiol Biochem, 4 (1): 11–9.
Keles MS, Taysi S, Sen N, Aksoy H, Akcay F (2001). Effect of corticosteroid therapy on serum and CSF malondialdehyde and antioxidant proteins in multiple sclerosis. Can J Neurol Sci, 28 (2):141-3.
Gomollon F, Gisbert JP (2009). Anemia and inflammatory bowel diseases. World J Gastroenterol, 15 (37): 4659–4665.
Glossop JR, Dawes PT, Hassell AB, Mattey DL (2005). Anemia in rheumatoid arthritis: association with polymorphism in the tumor necrosis factor receptor I and II genes. J Rheumatol, 32 (9): 1673–8.
Chan FK, Cryer B, Goldstein JL, Lanas A, Peura DA, et al (2010). A novel composite endpoint to evaluate the gastrointestinal (GI) effects of nonsteroidal antiinflammatory drugs through the entire GI tract. J Rheumatol, 37 (1): 167–74.
Beaumont C, Karim Z (2013). [Iron metabolism: State of the art]. Rev Med Interne, 34(1):17-25
Torti FM, Torti SV (2002). Regulation of ferritin genes and protein. Blood, 99 (10): 3505–16.
De Mutsert R, Grootendorst DC, Indemans F, et al (2009). Association between serum albumin and mortality in dialysis patients is partly explained by inflammation, and not by malnutrition. J Ren Nutr, 19 (2): 127-35.
Agarwal R, Davis JL, Smith L (2008). Serum albumin is strongly associated with erythropoietin sensitivity in hemodialysis patients. Clin J Am Soc Nephrol, 3 (1): 98-104.
Chan ES, Cronstein BN (2010). Methotrexate--how does it really work? Nat Rev Rheumatol, 6 (3):175–8.
Boechat AL, Oliveira CP, Tarragô AM, et al (2015). Methotrexate-loaded lipid-core nanocapsules are highly effective in the control of inflammation in synovial cells and a chronic arthritis model. Int J Nanomedicine, 10: 6603–14.
| Files | ||
| Issue | Vol 46 No 8 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| Hepatic proteins Inflammation Iron metabolism Rheumatoid arthritis | ||
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