Efficacy and Safety of G2013 as a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells

  • Nazanin ARJOMAND FARD Dept. of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Tehran, Iran
  • Nakisa TABRIZIAN Dept. of Cellular and Molecular Biology, Kish International Campus, University of Tehran, Tehran, Iran
  • Reza MIRZAEI Dept. of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Jamshid HADJATI Dept. of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Farzaneh TOFIGHI ZAVAREH Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Ali Reza SALEHI NODEH Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Abbas MIRSHAFIEY Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Keywords: NSAIDs, Anti-inflammatory agents, Dendritic cell, Immunosuppressive

Abstract

Background: The expanse of dendritic cells (DC) differentiation plays an important role in determining immune response. DC-based immunosuppressive drugs have notable side effects in increasing the risk of infectious diseases and cancers. G2013, as a novel anti-inflammatory and immunosuppressive agent, has been tested in experimental model of multiple sclerosis. The aim of this study was to conduct the safety property of G2013 on dendritic cells biology.Methods: The effect of G2013 on differentiation, maturation, and function of dendritic cells was examined at Tehran University in 2014. To investigate how G2013 affects human dendritic cells (DC) in a defined inflammatory environment, human peripheral blood mononuclear cells (PBMC) were isolated from healthy blood. Monocytes were then purified using anti-CD14 microbeads. Monocytes were treated with G2013 in two different doses (6 and 12 μg/well) along with adding granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 for inducing monocytes to immature DC and adding lipopolysaccharide for running DC maturation. Differentiation, maturation, and function of dendritic cells were examined with flow cytometry and ELISA.Results: G2013 therapy had no significant effect on CD83, CD86 and DR expression, as well as IL-10 and IL-12 cytokine levels and it, has no remarkable side on differentiation, maturation and function of dendritic cells in immature DC and mature DC process in vitro.Conclusion: G2013 is a safe agent with no adverse effect on differentiation, maturation, and function of dendritic cells. It may be recommended as a novel immunosuppressive agent with no or little side effect in increasing the risk of infectious diseases and cancers.  

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Published
2017-02-12
How to Cite
1.
ARJOMAND FARD N, TABRIZIAN N, MIRZAEI R, HADJATI J, TOFIGHI ZAVAREH F, SALEHI NODEH AR, MIRSHAFIEY A. Efficacy and Safety of G2013 as a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells. Iran J Public Health. 46(2):216-221.
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Original Article(s)