Bioremediation of Crude Oil Using Bacterium from the Coastal Sediments of Kish Island, Iran

  • Maryam SADEGHI HADDAD ZAVAREH Dept. of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
  • Gholamhossein EBRAHIMIPOUR Dept. of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
  • Mohsen SHAHRIARI MOGHADAM Dept. of Environment, Faculty of Natural Resources, University of Zabol, Zabol, Iran
  • Javad FAKHARI Dept. of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
  • Tahereh ABDOLI Dept. of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, G.C, Tehran, Iran
Keywords: Bioremediation, Halomonas sp, Taguchi, Growth kinetics, Iran

Abstract

Background: Much of the environment is affected by petroleum contamination. It imposes serious health problems for humans as well as serious environmental impact. Bioremediation is an important consideration for removing environmental pollutants because, compared with other technologies, it incurrs lower costs and is environmentally compatible.

Methods: Crude oil degrading bacteria were isolated using serial dilutions of a bacterial consortium. The Taguchi experimental design L16 (45) was used to optimize the biodegradation process of crude oil by the isolated strain. This investigation applied the parameters of temperature, salinity, pH, NH4Cl and FeSO4.7H2O. Modeling the kinetics of crude oil biodegradation included five batch cultivation experiments (2.5 ml/L to 40 ml/L) using crude oil as a single limiting substrate.

Results: Halomonas sp. MS1 was identified using identification tests. Maximum biodegradation efficiency was predicted to occur at pH=9, temperature=30 ˚C, salinity=2%, NH4Cl concentration=0.4 g/L and FeSO4.7H2O=0.04 g/L. After optimization, biodagradation was significantly (P<0.05) higher (i.e. 90.65%) than it results under the original conditions. Furthermore, growth kinetics modelling of bacteria in various concentrations of crude oil showed a positive correlation between increased concentration, up to 10 ml/L and bacterial growth, but this was not evident at higher concentrations (20-40 mL/L)

Conclusion: Overall, bacteria in surface sediment samples from Kish Island have been determined as having good potential for application in oil biodegradation. Optimum amounts of the studied factors were determined successfully by applying the Taguchi experimental design and the models of Teissier and Haldane are suggested as kinetic models to describe the batch crude oil degradation behavior of MS1.

 

References

Head IM, Jones DM, Roling WF (2006). Ma-rine microorganisms make a meal of oil. Nat Rev Microbiol, 4 (3): 173-182.

Hassanshahian M, Emtiazi G, Cappello S (2012). Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar Pollut Bull, 64 (1): 7-12.

Khan FI, Husain T, Hejazi R (2004). An overview and analysis of site remediation technologies. J Environ Manage, 71 (2): 95-122.

Madueno L, Coppotelli B, Alvarez H, Morelli I (2011). Isolation and characterization of indigenous soil bacteria for bioaugmentation of PAH contaminated soil of semiarid Patagonia, Argentina. Int Biodeterior Biodegrad, 65 (2): 345-351.

Zhou J, Yu X, Ding C, Wang Z, Zhou Q, et al. (2011). Optimization of phenol degradation by Candida tropicalis Z-04 using Plackett-Burman design and response surface methodology. J Environ Sci (China), 23 (1): 22-30.

Singh P, Parmar D, Pandya A (2015). Parametric optimization of media for crude oil degradation bacteria isolated from crude oil contaminated site. Int J Curr Microbiol App Sci, 4 (2): 322-328.

Atlas RM (1981). Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev, 45 (1): 180-209.

Salleh AB, Ghazali FM, Rahman R, Basri M (2003). Bioremediation of petroleum hydrocarbon pollution. Indian J Biotechnol, 2 (3): 411-425.

Margesin R, Schinner F (2001). Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl Microbiol Biotechnol, 56 (5-6): 650-663.

Aghamiri SF, Kabiri K, Emtiazi G (2011). A novel approach for optimization of crude oil bioremediation in soil by the taguchi method. J Pet Environ Biotechnol, 2 (2): 1-6.

Vays TK, Dave BP (2007). Effect of crude oil concentrations, temperature and pH on growth and degradation of crude oil by marine bacteria. Indian J Mar Sci, 36 (1): 76-85.

Mohajeri L, Aziz HA, Isa MH, Zahed MA (2010). A statistical experiment design approach for optimizing biodegradation of weathered crude oil in coastal sediments. Bioresour Technol, 101 (3): 893-900.

Roozbehani B, Sakaki A, Shishesaz M, Abdollahkhani N, Hamedifar S (2015). Taghuchi method approach on catalytic dgradation of polyethylene and polypropylene into gasolin. Clean Techn Environ Policy, 17 (7): 1873-1882.

Venkata Mohan S, Venkateswar Reddy M (2013). Optimization of critical factors to enhance polyhydroxyalkanoates (PHA) synthesis by mixed culture using Taguchi design of experimental methodology. Bioresour Technol, 128: 409-416.

Venkata MS, Sirisha K, Sreenivasa RR, Sarma P (2007). Bioslurry phase remediation of chlorpyrifos contaminated soil: process evaluation and optimization by Taguchi design of experimental (DOE) methodology. Ecotoxicol Environ Saf, 68 (2): 252-262.

Singh RK, Kumar S, Kumar S, Kumar A (2008). Biodegradation kinetic studies for the removal of p-cresol from wastewater using Gliomastix indicus MTCC 3869. Biochem Eng, 40 (2): 293-303.

Shahriari Moghadam M, Ebrahimipour G, Abtahi B, Khazaei N, Karbasi N (2014). Statistical Optimization of Crude Oil Biodegradation by Marinobacter sp. Isolated from Qeshm Island, Iran. Iran J Biotechnol, 12 (1): 35-41.

Mishra S, Jyot J, Kuhad RC, Lal B (2001). In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Curr Microbiol, 43 (5): 328-335.

Agarry S, Solomon B (2008). Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence. Int J Environ Sci Technol, 5 (2): 223-232.

Mnif S, Chamkha M, Sayadi S (2009). Isolation and characterization of Halomonas sp. strain C2SS100, a hydrocarbon‐degrading bacterium under hypersaline conditions. J Appl Microbiol, 107 (3): 785-794.

Vreeland R, Litchfield C, Martin E, Elliot E (1980). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Evol Bacteriol, 30 (2): 485-495.

Haddadi A, Shavandi M (2013). Biodegradation of phenol in hypersaline conditions by Halomonas sp. strain PH2-2 isolated from saline soil. Int Biodeterior Biodegrad, 85: 29-34.

Zhuang X, Han Z, Bai Z, Zhuang G, Shim H (2010). Progress in decontamination by halophilic microorganisms in saline wastewater and soil. Environ Pollut, 158 (5): 1119-1126.

Rao RS, Kumar CG, Prakasham RS, Hobbs PJ (2008). The Taguchi methodology as a statistical tool for biotechnological applications: a critical appraisal. Biotechnol J, 3 )4): 510-523.

Lin C, Gan L, Chen ZL (2010). Biodegradation of naphthalene by strain Bacillus fusiformis (BFN). J Hazard Mater, 182 (1-3): 771-7.

Das N, Chandran P (2011). Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnol Res Int, 2011: 941810.

Shahriari Moghadam M, Ebrahimipour G, Abtahi B, Ghassempour A (2013). Isolation, Identification and Optimization of Phenanthrene Degrading Bacteria From the Coastal Sediments of Nayband Bay. Jundishapur J Microbiol, 6 (9): e13816.

Shahriari Moghadam M, Ebrahimipour G, Abtahi B, Ghassempour A, Hashtroudi M (2014). Biodegradation of polycyclic aromatic hydrocarbon by a bacterial consortium enriched from mangrove sediments. J Environ Health Sci Eng, 12: 114.

Zaki MS, Mohammad MN Authman, Hossam HH Abbas (2015). Bioremediation of petroleum contaminants in aquatic environment. Life Sci J, 12(5): 127-139

Kumar M, Leon V, Materano ADS (2007). A halotolerant and thermotolerant Bacillus sp. degrades hydrocarbons and produces tensio-active emulsifying agent. World J Microbiol Biotechnol, 23 (2): 211-220.

Shiaris MP (1989). Seasonal biotransformation of naphthalene, phenanthrene, and benzo [a] pyrene in surficial estuarine sediments. Appl Environ Microbiol, 55 (6): 1391-1399.

Okpokwasili G, Nweke C (2005). Microbial growth and substrate utilization kinetics. Afr J Biotechnol, 5 (4): 305-317.

Wei YH, Chen WC, Chang SM, Chen BY (2010). Exploring kinetics of phenol biodegradation by Cupriavidus taiwanesis 187. Int J Mol Sci, 11 (12): 5065-5076

Ukpaka C (2011). Biodegradation model on effect of some physicochemical parameters on aromatic compounds in fresh water medium. J Bacteriol Res, 3 (3): 42-55.

Mathur A, Majumder C (2010). Kinetics modelling of the biodegradation of benzene, toluene and phenol as single substrate and mixed substrate by using Pseudomonas putida. Chem Biochem Eng Q, 24 (1): 101-10

Published
2016-05-25
How to Cite
1.
SADEGHI HADDAD ZAVAREH M, EBRAHIMIPOUR G, SHAHRIARI MOGHADAM M, FAKHARI J, ABDOLI T. Bioremediation of Crude Oil Using Bacterium from the Coastal Sediments of Kish Island, Iran. Iran J Public Health. 45(5):670-679.
Section
Original Article(s)