Molecular Characterization of Fasciola spp. from Some Parts of Iran
-
Hamid HASANPOUR
,
Reza FALAK
,
Saied Reza NADDAF
,
Santiago MAS-COMA
,
Mohammad Bagher ROKNI
,
Alireza BADIRZADEH
,
Kobra MOKHTARIAN
,
Mehdi MOHEBALI
,
Sanaz JAFARPOUR AZAMI
,
Arezoo FADAVI
,
Mohammad Javad GHARAGOZLOU
,
Kazem MOHAMMAD
,
Gholamreza MOWLAVI
Abstract
Background: Identification of liver flukes, Fasciola hepatica, and Fasciola gigantica by morphometric parameters is not always reliable due to the overlapping measurements. This study aimed to characterize the liver flukes of animals from different parts of Iran by the genetic markers, ITS1, and COXI.
Methods: We collected flukes from infected livestock in six provinces of Iran from Sep to Nov 2016. The flukes were identified by amplification of a 680 bp sequence of ITS1 locus followed by a restriction fragment polymorphism (RFLP) assay. The genetic diversity among isolates was evaluated by amplification and sequencing of a 493 bp fragment of the COXI gene.
Results: We obtained 38 specimens from Khuzestan, 22 from Tehran, 10 from Isfahan, 10 from Mazandaran, 4 from Kurdistan, and 3 from Ardabil provinces. PCR-RFLP analysis revealed two patterns, representing F. hepatica, and F. gigantica. Fifty specimens from cattle and sheep exhibited F. hepatica pattern and 37 from the cattle, sheep, buffalo, and goat that of F. gigantica. The phylogeny based on COXI revealed two distinct clades separating F. hepatica from F. gigantica. In our phylogeny, the Iranian F. gigantica isolates showed a distinct separation from the African flukes, while grouped with the East Asia specimens demonstrating a common ancestor. The F. hepatica isolates clustered with the flukes from different parts of the world, including East Asia, Europe, and South America.
Conclusion: The present study revealed a substantial genetic difference between F. gigantica populations of Asia and Africa, while F. hepatica isolates from different parts of the world shared high similarities.
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7. Eslami A, Hosseini S, Meshgi B (2009). An-imal fasciolosis in north of Iran. Iran. Iranian J Publ Health, 132-135.
8. Hosseini S, Meshgi B, Abbassi A, Eslami A (2012). Animal fascioliasis in coastal re-gions of the Caspian Sea, Iran (2006-2007). Iran J Vet Med, 13(1):64-7.
9. Shafiei R, Sarkari B, Sadjjadi SM et al (2014). Molecular and morphological characteri-zation of Fasciola spp. isolated from dif-ferent host species in a newly emerging focus of human fascioliasis in Iran. Vet Med Int, 2014:405740.
10. Sayadi M, Mohammad-Pourfard I, Yahyaei M, Esmaeili R (2015). The prevalence of fascioliasis in slaughtered animals of the industrial slaughterhouse of arak, Iran (2007-2010). Iran J Health Sci, 3(4):59-64.
11. Ashrafi K, Valero M, Panova M et al (2006). Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int, 55(4):249-60.
12. Ashrafi K (2015). The status of human and animal fascioliasis in Iran:A narrative re-view article. Iran J Parasitol, 10(3):306-328.
13. Hatami H, Asmar M, Masoud J et al (2012). The first epidemic and new-emerging human fascioliasis in Kermanshah (west-ern Iran) and a ten-year follow up, 1998-2008. Int J Prev Med, 3(4):266-72.
14. Heydarian P, Ashrafi K, Mohebali M et al (2017). Seroprevalence of human fasci-olosis in Lorestan Province, western Iran, in 2015–16. Iran J Parasitol, 12(3):3890-397.
15. Bozorgomid A, Nazari N, Eshrat Beigom K et al (2018). Epidemiology of fascioliasis in Kermanshah Province, western Iran. J Public Health, 47(7):967-972.
16. Sarkari B, Ghobakhloo N, Moshfea A, Eilami O (2012). Seroprevalence of hu-man fasciolosis in a new-emerging focus of fasciolosis in Yasuj district, southwest of Iran. Iran J Parasitol, 7(2):15-20.
17. Kheirandish F, Kayedi MH, Ezatpour B et al (2016). Seroprevalence of human fasci-olosis in Pirabad, Lorestan province, Western Iran. Iran J Parasitol, 11(1):24-29.
18. Peng M, Ichinomiya M, Ohtori M et al (2009). Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA. Parasitol Res,105(3):809-15.
19. Itagaki T, Kikawa M, Terasaki K, shibahara T, Fukuda K (2005). Molecular characteri-zation of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. J Vet Med Sci, 67(11):1115-8.
20. Itagaki T, Kikawa M, Sakaguchi K et al (2005). Genetic characterization of par-thenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology, 131(5):679-85.
21. Le TH, Van De N, Agatsuma T et al (2008). Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola he-patica and Fasciola gigantica in Vietnam. Int J Parasitol, 38(6):725-30.
22. Amor N, Halajian A, Farjallah S et al (2011). Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA se-quences. Exp Parasitol, 128(3):196-204.
23. Gupta A, Bhardwaj A, Sharma P, Pal Y (2015). Mitochondrial DNA-a tool for phylogenetic and biodiversity search in equines. J Biodivers Endanger Species, 2015. S1:S1006.
24. Aryaeipour M, Rouhani S, Bandehpour M et al (2014). Genotyping and phylogenetic analysis of Fasciola spp. isolated from sheep and cattle using PCR-RFLP in Ar-dabil province, northwestern Iran. Iran J Public Health, 43(10):1364-1371.
25. Ichikawa M, Itagaki T (2010). Discrimination of the ITS1 types of Fasciola spp. based on a PCR–RFLP method. Parasitol Res, 106(3):757-61.
26. Tamura K, Dudley J, Nei M, Kumar S (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software ver-sion 4.0. Mol Biol Evol, 24(8):1596-9.
27. Ghavami M, Rahimi P, Haniloo A, Mo-savinasab S (2009). Genotypic and phe-notypic analysis of Fasciola isolates. Iran J Parasitol, 61-70.
28. Reaghi S, Haghighi A, Harandi MF, Spotin A, Arzamani K, Rouhani S (2016). Mo-lecular characterization of Fasciola hepatica and phylogenetic analysis based on mito-chondrial (nicotiamide adenine dinucleo-tide dehydrogenase subunit I and cyto-chrome oxidase subunit I) genes from the North-East of Iran. Vet World, 9(9):1034-1038.
29. Saki J, Khademvatan S, Yousefi E (2011). Molecular identification of animal Fasciola isolates in southwest of Iran. Aust J Basic Appl Sci, 5(11):1878-83.
30. Bozorgomid A, Nazari N, Rahimi H et al (2016). Molecular characterization of ani-mal Fasciola spp. isolates from Kerman-shah, Western Iran. Iran. J Public Health, 45(10):1315-1321.
31. Nguyen S, Amer S, Ichikawa M et al (2012). Molecular identification of Fasciola spp.(Digenea: Platyhelminthes) in cattle from Vietnam. Parasite, 19(1):85-9.
2. Ai L, Chen M-X, Alasaad S et al (2011). Ge-netic characterization, species differentia-tion and detection of Fasciola spp. by mo-lecular approaches. Parasite Vectors, 4(1):101.
3. Mas-Coma S, Bargues MD, Valero M (2005). Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol, 35(11-12):1255-78.
4. Amer S, Dar Y, Ichikawa M et al (2011). Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mito-chondrial (NDI and COI) gene markers. Parasitol Int, 60(1):5-12.
5. Liu G-H, Gasser RB, Young ND et al (2014). Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F. hepatica. Parasite Vectors, 7(1):150.
6. Valero MA, Bargues MD, Khoubbane M et al (2016). Higher physiopathogenicity by Fasciola gigantica than by the genetically close F. hepatica: experimental long-term follow-up of biochemical markers. Trans R Soc Trop Med Hyg, 110(1):55-66.
7. Eslami A, Hosseini S, Meshgi B (2009). An-imal fasciolosis in north of Iran. Iran. Iranian J Publ Health, 132-135.
8. Hosseini S, Meshgi B, Abbassi A, Eslami A (2012). Animal fascioliasis in coastal re-gions of the Caspian Sea, Iran (2006-2007). Iran J Vet Med, 13(1):64-7.
9. Shafiei R, Sarkari B, Sadjjadi SM et al (2014). Molecular and morphological characteri-zation of Fasciola spp. isolated from dif-ferent host species in a newly emerging focus of human fascioliasis in Iran. Vet Med Int, 2014:405740.
10. Sayadi M, Mohammad-Pourfard I, Yahyaei M, Esmaeili R (2015). The prevalence of fascioliasis in slaughtered animals of the industrial slaughterhouse of arak, Iran (2007-2010). Iran J Health Sci, 3(4):59-64.
11. Ashrafi K, Valero M, Panova M et al (2006). Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran. Parasitol Int, 55(4):249-60.
12. Ashrafi K (2015). The status of human and animal fascioliasis in Iran:A narrative re-view article. Iran J Parasitol, 10(3):306-328.
13. Hatami H, Asmar M, Masoud J et al (2012). The first epidemic and new-emerging human fascioliasis in Kermanshah (west-ern Iran) and a ten-year follow up, 1998-2008. Int J Prev Med, 3(4):266-72.
14. Heydarian P, Ashrafi K, Mohebali M et al (2017). Seroprevalence of human fasci-olosis in Lorestan Province, western Iran, in 2015–16. Iran J Parasitol, 12(3):3890-397.
15. Bozorgomid A, Nazari N, Eshrat Beigom K et al (2018). Epidemiology of fascioliasis in Kermanshah Province, western Iran. J Public Health, 47(7):967-972.
16. Sarkari B, Ghobakhloo N, Moshfea A, Eilami O (2012). Seroprevalence of hu-man fasciolosis in a new-emerging focus of fasciolosis in Yasuj district, southwest of Iran. Iran J Parasitol, 7(2):15-20.
17. Kheirandish F, Kayedi MH, Ezatpour B et al (2016). Seroprevalence of human fasci-olosis in Pirabad, Lorestan province, Western Iran. Iran J Parasitol, 11(1):24-29.
18. Peng M, Ichinomiya M, Ohtori M et al (2009). Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA. Parasitol Res,105(3):809-15.
19. Itagaki T, Kikawa M, Terasaki K, shibahara T, Fukuda K (2005). Molecular characteri-zation of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. J Vet Med Sci, 67(11):1115-8.
20. Itagaki T, Kikawa M, Sakaguchi K et al (2005). Genetic characterization of par-thenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology, 131(5):679-85.
21. Le TH, Van De N, Agatsuma T et al (2008). Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola he-patica and Fasciola gigantica in Vietnam. Int J Parasitol, 38(6):725-30.
22. Amor N, Halajian A, Farjallah S et al (2011). Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA se-quences. Exp Parasitol, 128(3):196-204.
23. Gupta A, Bhardwaj A, Sharma P, Pal Y (2015). Mitochondrial DNA-a tool for phylogenetic and biodiversity search in equines. J Biodivers Endanger Species, 2015. S1:S1006.
24. Aryaeipour M, Rouhani S, Bandehpour M et al (2014). Genotyping and phylogenetic analysis of Fasciola spp. isolated from sheep and cattle using PCR-RFLP in Ar-dabil province, northwestern Iran. Iran J Public Health, 43(10):1364-1371.
25. Ichikawa M, Itagaki T (2010). Discrimination of the ITS1 types of Fasciola spp. based on a PCR–RFLP method. Parasitol Res, 106(3):757-61.
26. Tamura K, Dudley J, Nei M, Kumar S (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software ver-sion 4.0. Mol Biol Evol, 24(8):1596-9.
27. Ghavami M, Rahimi P, Haniloo A, Mo-savinasab S (2009). Genotypic and phe-notypic analysis of Fasciola isolates. Iran J Parasitol, 61-70.
28. Reaghi S, Haghighi A, Harandi MF, Spotin A, Arzamani K, Rouhani S (2016). Mo-lecular characterization of Fasciola hepatica and phylogenetic analysis based on mito-chondrial (nicotiamide adenine dinucleo-tide dehydrogenase subunit I and cyto-chrome oxidase subunit I) genes from the North-East of Iran. Vet World, 9(9):1034-1038.
29. Saki J, Khademvatan S, Yousefi E (2011). Molecular identification of animal Fasciola isolates in southwest of Iran. Aust J Basic Appl Sci, 5(11):1878-83.
30. Bozorgomid A, Nazari N, Rahimi H et al (2016). Molecular characterization of ani-mal Fasciola spp. isolates from Kerman-shah, Western Iran. Iran. J Public Health, 45(10):1315-1321.
31. Nguyen S, Amer S, Ichikawa M et al (2012). Molecular identification of Fasciola spp.(Digenea: Platyhelminthes) in cattle from Vietnam. Parasite, 19(1):85-9.
| Files | ||
| Issue | Vol 49 No 1 (2020) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijph.v49i1.3062 | |
| Keywords | ||
| Fasciola gigantica Iran | ||
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How to Cite
1.
HASANPOUR H, FALAK R, NADDAF SR, MAS-COMA S, ROKNI MB, BADIRZADEH A, MOKHTARIAN K, MOHEBALI M, JAFARPOUR AZAMI S, FADAVI A, GHARAGOZLOU MJ, MOHAMMAD K, MOWLAVI G. Molecular Characterization of Fasciola spp. from Some Parts of Iran. Iran J Public Health. 2020;49(1):157-166.
