Association of IL-1B+3954 and IL-1RN Polymorphisms in Chronic Gastritis and Peptic Ulcer
AbstractAbstractBackground: Helicobacter pylori are the main cause of chronic inflammation and peptic ulcer. We aimed to determine if IL-1B+3954 and IL-1RN polymorphisms are associated with the risk of chronic gastritis and peptic ulcer in Iranian population. Methods: In this case-control study, from 198 individuals enrolled by Mohammadi Hospital, Bandar Abbas, southern Iran from 2012 to 2014 and who showed the symptoms of chronic gastritis and 84 with peptic ulcer participated in the case group, two biopsies were taken from the body, antrum, or ulcer edge of each patient. Individuals without chronic gastritis or peptic ulcer were selected as the control group and we also confirmed the presence of anti-H. pylori serum IgG in 321 control subjects. IL-1B+3954C/T polymorphism was analyzed through PCR-RFLP, while the IL-1RN polymorphism was analyzed via PCR-based VNTR.Results: IL-1B+3954 TT was associated with a high risk of gastritis and peptic ulcer [Odds Ratio (OR)]=2.63, 95% Confidence Interval (CI)= (1.47-4.70) (OR=3.40, CI=1.72-6.71) respectively and the IL-1B+3954 T allele was associated with chronic gastritis (OR=1.64, 95% CI=1.13-2.36). Moreover, patient carrying IL-1RN L/2 and allele 2 showed an increased risk of peptic ulcer (OR=2.97, CI=1.72-5.11, OR=1.64, CI=1.13-2.36), respectively.Conclusion: IL-1B and IL-1RNare associated with an increased risk for chronic gastritis and peptic ulcer disease.
Barbosa HPM, Martins LC, dos Santos SEB, et al (2009). Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population. World J Gastroenterol, 15 (12): 1465-71.
Qadri Q, Rasool R, Afroze D, et al (2014). Study of TLR4 and IL-8 Gene Polymorphisms in H. pylori-Induced Inflammation in Gastric Cancer in an Ethnic Kashmiri Population. Immunol Invest, 43 (4): 324-36.
Chen A, Li CN, Hsu PI, et al (2004). Risks of interleukin‐1 genetic polymorphisms and Helicobacter pylori infection in the development of gastric cancer. Aliment Pharmacol Ther, 20 (2): 203-11.
Rad R, Prinz C, Neu B, et al (2003). Synergistic effect of Helicobacter pylori virulence factors and interleukin-1 polymorphisms for the development of severe histological changes in the gastric mucosa. J Infect Dis, 188 (2): 272-81.
Wang YM, Li ZX, Tang FB, et al (2016). Association of genetic polymorphisms of interleukins with gastric cancer and precancerous gastric lesions in a high-risk Chinese population. Tumour Biol, 37 (2): 2233-42.
Peek RM, Blaser MJ (2002). Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer, 2 (1): 28-37.
Yin YW, Hu AM, Sun QQ, et al (2013). Association between interleukin-8 gene-251 T/A polymorphism and the risk of peptic ulcer disease: A meta-analysis. Hum Immunol, 74 (1): 125-30.
Kumar S, Kumari N, Mittal RD, et al (2015). Association between pro-(IL-8) and anti-inflammatory (IL-10) cytokine variants and their serum levels and H. pylori-related gastric carcinogenesis in northern India. Meta Gene, 6: 9-16.
Dinarello CA (1996). Biologic basis for interleukin-1 in disease. Blood, 87 (6): 2095-147.
Ramis IB, Vianna JS, Halicki PCB, et al (2015). Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis. J Infect Dev Ctries, 9 (10): 1108-16.
Lee SG, Kim B, Choi W, et al (2003). Lack of association between pro-inflammatory genotypes of the interleukin-1 (IL-1B-31 C/+ and IL-1RN* 2/* 2) and gastric cancer/duodenal ulcer in Korean population. Cytokine, 21 (4): 167-71.
GarciaGonzalez M, Lanas A, Santolaria S, et al (2001). The polymorphic IL‐1B and IL‐1RN genes in the aetiopathogenesis of peptic ulcer. Clin Exp Immunol, 125 (3): 368-75.
Manfred Stolte M (2001). The updated Sydney system: classification and grading of gastritis as the basis of diagnosis and treatment. Can J Gastroenterol, 15 (9): 591-8.
Pociot F, Mølvig J, Wogensen L, et al (1992). A Taql polymorphism in the human interleukin‐1β (IL‐1β) gene correlates with IL‐1β secretion in vitro. Eur J Clin Invest, 22 (6): 396-402.
He B, Pan Y, Xu Y, et al (2012). Increased Risk for Gastric Cancer in Carriers of the Lymphotoxin-α+ 252G Variant Infected by Helicobacter pylori. Genet Test Mol Biomarkers, 16 (1): 9-14.
Zhang BB, Wang J, Bian DL, Chen X-Y (2012). No association between IL-1β-31 C/T polymorphism and the risk of duodenal ulcer: a meta-analysis of 3,793 subjects. Hum Immunol, 73 (11): 1200-6.
He BS, Pan YQ, Xu YF, et al (2011). Polymorphisms in interleukin-1B (IL-1B) and interleukin 1 receptor antagonist (IL-1RN) genes associate with gastric cancer risk in the Chinese population. Dig Dis Sci, 56 (7): 2017-23.
Resende RG, Guimarães Abreu MHN, de Souza LN, et al (2013). Association between IL1B (+ 3954) polymorphisms and IL-1β levels in blood and saliva, together with acute graft-versus-host disease. J Interferon Cytokine Res, 33 (7): 392-7.
ElOmar EM, Carrington M, Chow WH, et al (2000). Interleukin-1 polymorphisms associated with increased risk of gastric cancer. Nature, 404 (6776): 398-402.
Hnatyszyn A, Wielgus K, Kaczmarek Rys M, et al (2013). Interleukin-1 gene polymorphisms in chronic gastritis patients infected with Helicobacter pylori as risk factors of gastric cancer development. Arch Immunol Ther Exp (Warsz), 61 (6): 503-12.
Zabaleta J, Camargo MC, Piazuelo MB, et al (2006). Association of interleukin-1β gene polymorphisms with precancerous gastric lesions in African Americans and Caucasians. Am J Gastroenterol, 101 (1): 163-71.
Zeng ZR, Hu PJ, Hu S et al (2003). Association of interleukin 1B gene polymorphism and gastric cancers in high and low prevalence regions in China. Gut, 52 (12): 1684-9.
Peleteiro B, Lunet N, Carrilho C, et al (2010). Association between cytokine gene polymorphisms and gastric precancerous lesions: systematic review and meta-analysis. Cancer Epidemiol Biomarkers Prev, 19 (3): 762-76.
Xue H, Lin B, Ni P, et al (2010). Interleukin‐1B and interleukin‐1 RN polymorphisms and gastric carcinoma risk: A meta‐analysis. J Gastroenterol Hepatol, 25 (10): 1604-17.
Rocha GA, Guerra JB, Rocha AMC, et al (2005). IL1RN polymorphic gene and cagA‐positive status independently increase the risk of noncardia gastric carcinoma. Int J Cancer, 115 (5): 678-83.