Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 30 2251 2259 Natalija Djurovic Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Snezana Radovanovic 1. Department of Social Medicine, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia 2. Institute for Public Health Kragujevac, Kragujevac, Serbia 3. Center for Research on Harmfull Effects of Biological and Chemical Hazards, Faculty of Medical Sciences, University of Kragu-jevac, Kragujevac, Serbia Olgica Mihaljevic Department of Pathophysiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Jovana Radovanovic Department of Epidemiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Milos Stepovic Department of Anatomy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Marija Kovacevic Department of Anatomy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Katarina Janicijevic Department of Social Medicine, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Gordana Djordjevic Department of Epidemiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Ognjen Djordjevic Department of Epidemiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Gordana Gajovic Health Center Arandjelovac, Arandjelovac, Serbia Nela Djonovic 1. Institute for Public Health Kragujevac, Kragujevac, Serbia 2. Department of Hygiene and Ecology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Tamara Dimovic Institute for Public Health Kragujevac, Kragujevac, Serbia Nikoleta Janicijevic Department of Hygiene and Ecology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Milena Maricic The College of Health Sciences, Academy of Applied Studies Belgrade, Belgrade, Serbia Dragan Vasiljevic Department of Hygiene and Ecology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia Viktor Selakovic Department of Communication Skills, Ethics and Psychology, Faculty of Medical Sciences, University Kragujevac, Kragujevac, Saerbi 2024 07 10 2024 09 30 Background: Social networks and support have an independent and strong influence on health and quality of life at all stages of the life cycle, especially in the elderly. We aimed to determine the level of social support among the elderly population of the Republic of Serbia. Methods: The survey is part of the fourth Health survey of the population of Serbia, which was conducted in 2019  by the Republic Institute of Statistics with the Institute for Public Health of Serbia "Dr. Milan Jovanović Batut" and the Ministry of Health of the Republic of Serbia. The research instrument was standardized questionnaires constructed in accordance with the European Health Interview Survey (EHIS, wave3) and the social support score (Oslo-3 Social Support Scale). For the purposes of this research, data on the population aged 65 and over were used. Results: In terms of social support, 85.8% of respondents had bad , 13.9% moderate and 0.3% strong. Univariate regression analysis determined that respondents from the territory of Vojvodina had a 1.6 times higher chance of poor social support compared to respondents from southern and eastern Serbia.  The probability of bad social support was 1.5 times higher in persons who were never married/cohabiting, persons with secondary education were at 1.6 times higher risk of poor social support compared to persons with higher education. Inactive persons had poor social support 1.4 times more often than employed persons, as well as persons with comorbidities (OR=1.218). Conclusion: These researches are of great importance for public health because it provides valuable data for the creation of interventions that will intensify activities related to providing adequate social support to the elderly. https://ijph.tums.ac.ir/index.php/ijph/article/view/36040 https://ijph.tums.ac.ir/index.php/ijph/article/download/36040/8365

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 29 2167 2179 Junhua Wang Department of Cardiovascular Medicine, Taixing People's Hospital, Taizhou, Jiangsu, China Hui Li Department of Medicine, Taixing People's Hospital, Taizhou, Jiangsu, China Wenjuan Yan Department of Cardiovascular Medicine, Taixing People's Hospital, Taizhou, Jiangsu, China Na Xue Department of Cardiovascular Medicine, Taixing People's Hospital, Taizhou, Jiangsu, China Jin Yin Department of Nursing, Taixing People's Hospital, Taizhou, Jiangsu, China Nawsherwan . Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China Sha Yin Department of Cardiovascular Medicine, Taixing People's Hospital, Taizhou, Jiangsu, China 2024 07 02 2024 09 29 . https://ijph.tums.ac.ir/index.php/ijph/article/view/35948 https://ijph.tums.ac.ir/index.php/ijph/article/download/35948/8358

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 30 2380 2381 Sang-Hyuk Park Division of Sport Science, College of Arts & Physical Education, Incheon National University, Incheon, Republic of Korea Seung-Taek Lim 1. General Education, Kookmin University, Seoul, Republic of Korea 2. Waseda Institute for Sport Sciences, Waseda University, Saitama, Japan 2024 03 29 2024 09 30 No Abstract No Abstract No Abstract. https://ijph.tums.ac.ir/index.php/ijph/article/view/35011 https://ijph.tums.ac.ir/index.php/ijph/article/download/35011/8378

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 30 2260 2270 Yan Gu Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Rongjin Chen Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Jianxiang Song Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Zhan Shi Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Jixiang Wu Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Huiwen Chang Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Conghu Yuan Department of Anesthesiology, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hospital, Yan-cheng, Jiangsu 224000, China Woda Shi Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China Yajun Zhang Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Nantong University, Yancheng Third People's Hos-pital, Yancheng, Jiangsu 224000, China 2023 11 23 2024 09 30 Background: Calcific aortic valve disease (CAVD) is the most common heart relating disease with high morbidity and mortality, especially in elderly population. While extensive investigations have been devoted to the study of mechanistic pathways related to CAVD, the key factors and mechanisms mediating valve mineralization remain unclear. The aim of this study is to investigate the role of mirnas and their downstream targets in CAVD disease progression. A previous recent multi-omics study suggested a novel CAVD molecular interaction network contained miR-21-5p. Methods: CAV and their pair-matched adjacent normal tissues were obtained from 15 patients pathologically diagnosed as CAVD and admitted in Yancheng Third People's Hospital (The Sixth Affiliated Hospital of Nantong University) from 2019-2021. RT-qPCR was utilized for detection of miR-21-5p and related protein expression levels to confirm the related factors in CAVD progression. Western blotting was applied to strengthen the results of RT-qPCR and confirm osteogenic differentiation of VICs via biomarker detection. The staining of alkaline phosphatase (ALP) and alizarin red was performed to assess the degree of VIC mineralization. Results: We found that miR-21-5p was remarkably increased (P<0.0001) in calcified aortic valves (AVs) whereas TGFBI was diminished (P<0.01) in CAVD samples compared to the paired normal tissues from CAVD patients. Additionally, TGFBI was targeted by miR-21-5p. Furthermore, overexpressing TGFBI could block VIC osteogenic differentiation mediated by miR-21-5p. To sum up, miR-21-5p promotes VIC osteogenic differentiation and calcification via TGFBI in CAVD progression. Conclusion:  Our work might bring a sight on underlying mechanisms of CAVD progression and provide a possible therapeutic target for diagnosis and treatment. https://ijph.tums.ac.ir/index.php/ijph/article/view/33840 https://ijph.tums.ac.ir/index.php/ijph/article/download/33840/8366

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 30 2180 2190 EN Nurgul Sikhayeva National Center for Biotechnology, Astana, 010000, Kazakhstan Akmaral Nursafina Faculty of Natural Science, Eurasian National University Named After L.N. Gumilyov, Astana, 010000, Kazakhstan Aidana Satayeva Faculty of Natural Science, Eurasian National University Named After L.N. Gumilyov, Astana, 010000, Kazakhstan Talgat Utupov National Center for Biotechnology, Astana, 010000, Kazakhstan 2023 08 16 2024 01 15 Background: Thirty-two single nucleotide polymorphisms (SNPs) are commonly found in ADIPOQ. APN levels are decreased in obesity, and SNPs of the ADIPOQ gene affecting APN have varying associations with the development of obesity in different populations. This systematic review and meta-analysis aimed to investigate the association of SNPs in ADIPOQ with the risk of obesity development in various populations. Methods: A systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist date up to Feb 2023. We used the Newcastle–Ottawa scale to find out if a study fit the main criteria for submission and to assess the data quality of the articles included in the systematic review and meta-analysis. Odds ratios (OR) and 95% confidence intervals (CI) were calculated via Review Manager (RM) V.5.4 to estimate the connection between ADIPOQ polymorphic qualities of a gene and the risk of developing obesity. Results: The present study analysed the association between ADIPOQ polymorphisms (rs1501299, rs2241766, rs266729, rs822393, and rs822396) and obesity risk and suggested that APN is partially responsible for the emergence of obesity and increases its risk. Conclusion: It is important to take into account several limitations of this meta-analysis when evaluating the findings. First off, even though we looked through numerous databases for all relevant papers, there is a chance we overlooked some. Our capacity to arrive at more firm conclusions was further hampered by the small number of papers that made up our meta-analysis. The most current data, however, are presented in this study since it used newly published data to perform a meta-analysis and evaluate the relationship between ADIPOQ polymorphisms and obesity. https://ijph.tums.ac.ir/index.php/ijph/article/view/32922 https://ijph.tums.ac.ir/index.php/ijph/article/download/32922/8359

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 30 2382 2384 Masoumeh Roohaninasab Department of Dermatology, Rasool Akram Medical Complex, Iran University of Medical Sciences, Tehran, Iran Safoura Shakoei Department of Dermatology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Azadeh Goodarzi Department of Dermatology, Rasool Akram Medical Complex, Iran University of Medical Sciences, Tehran, Iran Parvin Mansouri Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran Farahnaz Nikkhah Department of Rasool Akram Medical Complex Clinical Research Development Center, Iran University of Medical Sciences, Tehran, Iran 2021 05 27 2022 02 23 No Abstract No Abstract No Abstract https://ijph.tums.ac.ir/index.php/ijph/article/view/25092 https://ijph.tums.ac.ir/index.php/ijph/article/download/25092/8379

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 10 2024 09 30 dical University', Astana, Kazakhstan Alexandr FURSOV JSC 'Astana Medical University', Astana, Kazakhstan Bagdat IMASHEVA JSC 'Astana Medical University', Astana, Kazakhstan Roman FURSOV JSC 'Astana Medical University', Astana, Kazakhstan Yerzhan KUSPAEV JSC 'Astana Medical University', Astana, Kazakhstan Timofei KOVALENKO JSC 'Astana Medical University', Astana, Kazakhstan Alishan ISMAILOV JSC 'Astana Medical University', Astana, Kazakhstan Aigul ABDULDAYEVA JSC 'Astana Medical University', Astana, Kazakhstan Irina VOLCHKOVA JSC 'Astana Medical University', Astana, Kazakhstan 2019 10 04 Background: The prevalence of bleeding and perforation against the diabetes mellitus, obesity and metabolic syndrome (MetS) is studied not sufficiently. Methods: The period of collecting the material was 15 years (2003-2017). The observation analysis was conducted for the patients at polyclinics observations, by the doctors of first-aid, in the surgical departments of the Astana City, Kazakhstan. The number of first-aid visits to patients, the medical cards of the patients with gastroduodenal perforation (GDP) and gastroduodenal bleeding (GDB) were analyzed. Results: The rate of annual growth of indices of overall morbidity Rgm=1.0%, obesity in combination with diabetes was Ro=1.7%; and morbidity with metabolic syndrome was Rm=3.1%. The diagnosis of GDP was revealed in 0.63 men with MetS for every 100000 urban people. The diagnosis of GDB was registered in 2.12 men for 100000 urban people. The index of the annual growth in patients with MetS had the tendency to the growth (Rms=3.1%). Conclusion: The high medical and social significance of diseases of the digestive system among the adult population in Kazakhstan is determined by the annual increase in the incidence rate and a clear decrease in the quality of life of such patients, which necessitates the search for scientifically based ways to improve medical care for this population. https://ijph.tums.ac.ir/index.php/ijph/article/view/18533 https://ijph.tums.ac.ir/index.php/ijph/article/download/18533/6530

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1924 1926 Jeong-Hyun LEE Department of Beauty Art, Youngsan University, Busan, Republic of Korea Yeona KIM Department of Beauty Art, Youngsan University, Busan, Republic of Korea Wi-Young SO Sports and Health Care Major, Korea National University of Transportation, Chungju-si, Republic of Korea 2019 10 05 Abstracts Abstracts  Abstracts  Abstracts https://ijph.tums.ac.ir/index.php/ijph/article/view/18560 https://ijph.tums.ac.ir/index.php/ijph/article/download/18560/6550

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1794 1801 Dariusz BOGUSZEWSKI Department of Rehabilitation, Physiotherapy Division, 2nd Faculty of Medicine, Medical University of Warsaw, Warsaw, Poland Jakub Grzegorz ADAMCZYK Department of Theory of Sport, Józef Pilsudski University of Physical Education in Warsaw, Warsaw, Poland Dariusz BIALOSZEWSKI Department of Rehabilitation, Physiotherapy Division, 2nd Faculty of Medicine, Medical University of Warsaw, Warsaw, Poland 2019 10 05 Background: Physical activeness is one of the main elements of lifestyle in terms of preventing civilization diseases. The main purpose of this study was to assess the selected health behaviors of men practicing combat sports and martial arts and to find out what features or variables may have an influence one’s lifestyle. Methods: Overall, 561 men aged 17-35 yr were enrolled and divided into three groups. Studies were conducted from March 2011 to June 2015 in Warsaw, Poland. The main research tool was Juczyński’s Health Behaviour Inventory. The questionnaire includes 24 statements – descriptions of various health-related attitudes and behaviours – divided into four categories: proper eating habits, preventive behaviours, positive psychical attitude, and health practices. Results: The persons practicing combat sports and martial arts presented the highest level of health behaviours. The result of Group 1 was significantly higher than the result of Group 2 (P=0.05) and Group 3 (P<0.001). The sportsmen from Group 1 obtained the highest result in the category of psychical attitude (3.5), which was similar to the result of the non-active group (3.29). The men belonging to Group 2 rated highest their eating habits (3.37). All groups obtained the lowest results in the category of preventive behaviours. Conclusion: The men practicing combat sports and martial arts showed high general level of health behaviours. It may give evidence to the intellectualization of sport and health training process, as well as to the fact of adopting the desired behaviours in everyday life.     https://ijph.tums.ac.ir/index.php/ijph/article/view/18536 https://ijph.tums.ac.ir/index.php/ijph/article/download/18536/6531

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1927 1929 Jinkui LU School of Sport, Shangrao Normal University, Jiangxi Shangrao, 334000, P.R. China Yongsheng XU School of Sport, Shangrao Normal University, Jiangxi Shangrao, 334000, P.R. China Jianming XIANG School of Sport, Shangrao Normal University, Jiangxi Shangrao, 334000, P.R. China 2019 10 05 No Abstract No Abstract No Abstract https://ijph.tums.ac.ir/index.php/ijph/article/view/18561 https://ijph.tums.ac.ir/index.php/ijph/article/download/18561/6551

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1802 1809 Lili ZHUANG Reproductive Department, Yantai Yuhuangding Hospital, Yantai 264000, P.R. China Wei CUI Obstetrics Department, Yantai Yuhuangding Hospital, Yantai 264000, P.R. China Jianxiang CONG Reproductive Department, Yantai Yuhuangding Hospital, Yantai 264000, P.R. China Yinghong ZHANG Obstetrics Department, Yantai Yuhuangding Hospital, Yantai 264000, P.R. China 2019 10 05 Background: We aimed to explore the clinical efficacy of vitamin D combined with metformin and clomiphene in the treatment of patients with polycystic ovary syndrome combined with infertility. Methods: Overall, 396 cases of polycystic ovarian syndrome combined with infertility in Yantai Yuhuangding Hospital, Yantai, China were prospectively analyzed. Among them, 204 cases treated with vitamin D combined with metformin and clomiphene were set as the study group; 192 cases treated with only metformin and clomiphene were set as the control group. The ovarian volume and thickness of uterine wall before and after treatment were recorded. Levels of fasting insulin (FINS), luteinizing hormone (LH), testosterone (T), follicle-stimulating hormone (FSH), and estradiol (E2) before and after treatment were recorded. Results: There was no difference in body weight, BMI, ovarian volume, thickness of ovarian wall, FINS, LH, T, FSH and E2 between the study group and the control group; there was no significant difference in FSH, E2 and the thickness of uterine wall between the two groups. After treatment, the BMI, FINS, LH, and T in the study group were significantly lower than those in the control group (P<0.05); the incidence rates of oligomenorrhea, facial acne and hairy symptoms in the study group were significantly lower than those in the control group (P<0.05); after treatment, the ovulation rate and pregnancy rate in 36 cycles in the observation group were significantly higher than those of the control group (P<0.05). Conclusion: Our challenge could significantly improve clinical symptoms and endocrine conditions, and greatly enhance the ovulation rate and pregnancy rate.  https://ijph.tums.ac.ir/index.php/ijph/article/view/18537 https://ijph.tums.ac.ir/index.php/ijph/article/download/18537/6532

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1930 1932 Min ZHANG Department of Neurosurgery, The First Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161041, P.R. China Yang YU Department of Neurology, The First Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161041, P.R. China Yujun CHEN Department of Neurosurgery, The First Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161041, P.R. China Guihong FAN Department of Nursing, The First Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161041, P.R. China 2019 10 05 Abstract Abstract Abstract Abstract Abstract https://ijph.tums.ac.ir/index.php/ijph/article/view/18563 https://ijph.tums.ac.ir/index.php/ijph/article/download/18563/6552

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1810 1816 Gozde SERİNDERE Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Hatay Mustafa Kemal University, Hatay, Turkey Behiye BOLGUL Department of Pedodontics, Faculty of Dentistry, Hatay Mustafa Kemal University, Hatay, Turkey Didar GURSOY Department of Pathology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey Sibel HAKVERDİ Department of Pathology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey Nazan SAVAS Department of Public Health, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey 2019 10 05 Background: Although oral health improves in several countries, global problems are still present. Predictably, the disadvantaged and poor population groups in both developing and developed countries have high rate of malign disease. The aim of this study was to evaluate the prevalence of head and neck cancers (HNCs) and to compare them between Syrian and Turkish population. Methods: A total of 4570 patients confirmed to have HNC histopathologically from Hatay Mustafa Kemal University Hospital Pathology report archive were retrospectively evaluated. Among them, 452 were Syrian patients while 4118 were Turkish patients. Data were collected from 2010 to 2017. Gender and age information were taken from medical records. According to the pathological results, HNCs were classified. Results: In 474 patients, HNCs were inscriptived, of which 317 were in males and 157 in females aged 23–80 years with histologically approved cancer of head and neck area. Overall, 100 were Syrian patients while 374 were Turkish patients. In both Syrian and Turkish patients, the most observed HNC was squamous cell carcinoma (SCC). Conclusion: Nowadays, the prevalence of cancer is higher because of the excessive consumption of alcohol, tobacco, chewing, and smoking. For the higher cancer incidence in Syrian refugees, we thought that the impact of war such as stress may have been effective as well as the known several etiologic factors of cancer. For the increased risk of cancer, the early diagnosis of this become more important. https://ijph.tums.ac.ir/index.php/ijph/article/view/18538 https://ijph.tums.ac.ir/index.php/ijph/article/download/18538/6533

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1933 1934 Rui ZHAO Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 611756, China 2019 10 05 Abstract Abstract Abstract Abstract Abstract https://ijph.tums.ac.ir/index.php/ijph/article/view/18564 https://ijph.tums.ac.ir/index.php/ijph/article/download/18564/6553

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1817 1826 Ye LI Department 3 of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science, Wuhan 430034, P.R. China Jie TANG Department 3 of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science, Wuhan 430034, P.R. China Yong HU Department 3 of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science, Wuhan 430034, P.R. China Yonghai PENG Department 3 of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science, Wuhan 430034, P.R. China Junwen WANG Department 2 of Orthopedics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science, Wuhan 430034, P.R. China 2019 10 05 Background: To explore the expression level of miR-664-3p in osteosarcoma and its effects on the proliferation and apoptosis of osteosarcoma cells. Methods: Specimens of osteosarcoma tissues were collected from 41 cases undergoing surgical treatment in the Orthopedics Department of Wuhan Puai Hospital, Wuhan, China from January 2015 to February 2018. Another 40 cases of normal bone tissue were collected. The expression of miR-664-3p were detected using quantificational real-time polymerase chain reaction. miR-664-3p mimics, miR-664-3p inhibitor and miR-664-3p negative control (NC) were used to transfect U2-OS, which were named as mimics group, inhibitor group and NC group, respectively. MTT assay was adopted to detect the effects of microRNA-664-3p on the proliferation of U2-OS after 24, 48, 72, 96 and 120 hours of transfection. Flow cytometry was applied to measure the apoptosis rate of U2-OS after miR-664-3p transfection. Finally, Western Blot was employed to detect the expression of proteolipid protein 2 (PLP2). Results: The total apoptosis rate of cells in the inhibitor group was obviously higher than those in the mimics group and the NC group (P<0.001). The relative expression level of PLP2 in the inhibitor group was significantly lower than those in the mimics group and the NC group (P<0.001). Conclusion: MiR-664-3p may be involved in the occurrence and development of osteosarcoma, and can regulate the proliferation and apoptosis of U2-OS cells, and the expression of PLP2. Besides, miR-664-3p may become a novel molecular biological indicator for the diagnosis, targeted treatment and prognosis assessment of osteosarcoma. https://ijph.tums.ac.ir/index.php/ijph/article/view/18539 https://ijph.tums.ac.ir/index.php/ijph/article/download/18539/6534

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1935 1936 Ahmad KALATEH SADATI Department of Sociology, Yazd University, Yazd, Iran Kamran BAGHERI LANKARANI Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran 2019 10 05 Abstract Abstract Abstract Abstract Abstract https://ijph.tums.ac.ir/index.php/ijph/article/view/18565 https://ijph.tums.ac.ir/index.php/ijph/article/download/18565/6554

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1827 1837 Elham TAGHAVI Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Ramin DANESHVAR Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran AND Genetic Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Zahra NOORMOHAMMADI Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Seyed Mohammad-Hossein MODARRESI Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza SEDAGHAT Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran 2019 10 05 Background: Pseudoexfoliation syndrome (XFS) is pathogenetically related to exfoliative glaucoma (XFG), which is the most common type of secondary glaucoma. We aimed to investigate the relationship between LOXL1 SNPs (rs1048661, rs3825942) and XFS and/or XFG in a cohort of Iranian subjects. Methods: This cross-sectional study investigated possible association between LOXL1 gene polymorphisms and exfoliative glaucoma in Northeastern part of Iran between May 2014 and May 2015. Sixty unrelated XFS/XFG patients, as well as 40 control subjects, were studied by direct sequencing. In fifteen senile cataract patients without glaucoma and fifteen patients with coexisting XFG and cataract, capsulorhexis specimen of the anterior lens capsule was used to evaluate LOXL1 gene transcripts by Real-Time PCR technique. We analyzed the results for allele frequencies and haplotype association and investigated the relative gene expression. Results: Significant associations between the rs382594 SNP and XFG and between rs1048661 SNP and XFG were observed (P<0.05 for both). The frequency of the G allele in the exonic SNP (rs1048661) appeared to be higher in XFS or XFG patients compared to control subjects (P= 0.0497). Moreover, in the rs3825942 SNP, the G allele was more frequent in XFS/XFG patients compared to control subjects (P=0.0016). The highest cumulative frequency was for the GG haplotype. GG haplotype was associated with increased risk of XFG compared to the rs1048661 G/T and rs3825942 G/A haplotypes. LOXL1 mRNA expression was not statistically significantly different between XFS/XFG and control subjects. Conclusion: We reported the LOXL1 gene polymorphism in an Iranian XFS/XFG cohort. Similar to many other ethnic groups and geographic regions, our results confirmed an association between LOXL1 gene variants and XFG in Iran. https://ijph.tums.ac.ir/index.php/ijph/article/view/18540 https://ijph.tums.ac.ir/index.php/ijph/article/download/18540/6535

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1937 1939 Seyyed Ali MOEZI Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Nahid AZDAKI Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Toba KAZEMI Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Hamid Reza MASHREGHI MOGHADDAM Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Neda PARTOVI Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Fatemeh HAMIDI Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Nazanin HANAFI-BOJD Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Majid JAFARNEJAD Cardiovascular Diseases Research Center, Department of Cardiology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran Samaneh NAKHAEE Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences, Birjand, Iran AND Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran 2019 10 05 Abstract Abstract Abstract Abstract Abstract https://ijph.tums.ac.ir/index.php/ijph/article/view/18566 https://ijph.tums.ac.ir/index.php/ijph/article/download/18566/6555

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1838 1846 Ehsan GHAEDI Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Fatemeh RAHROVANI Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Mohammad Hassan JAVANBAKHT Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Amir-Hooshang EHSANI Department of Dermatology, Autoimmune Bullous Diseases Research Center, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran Ali ESRAFILI Department of Environmental Health Engineering, School of Public Health, Iran University of Medical Sciences, Tehran, Iran Hamed MOHAMMADI Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran AND Students’ Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran Mahnaz ZAREI Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran Mahmoud DJALALI Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran 2019 10 05 Background: Nonmelanoma skin cancers are the most frequently occurring skin cancers. Vitamin A is involved in epithelial cell differentiation and may control skin tumor development. Vitamin E is a powerful lipophilic antioxidant that can quench and scavenge reactive oxygen species. However, there is little consistent evidence considering micronutrients and the development of basal cell carcinoma (BCC). Therefore, we aimed to investigate the possible difference between retinol and α-tocopherol in BCC patients and controls in Iranian population. Methods: This case-control study was conducted on adults with newly diagnosed BCC referred to Razi Hospital, Tehran, Iran in 2015. Serum and subcutaneous fat tissue retinol and α-tochopherol were measured by HPLC method. Results: Overall, serum retinol level was lower significantly in BCC patients (0.237±0.01 µg/ml) in comparison with control group (0.27±0.02 µg/ml, P-value: 0.038). However serum ɑ-tocopherol level was not significantly different between BCC patients (4.41±0.33 µg/ml) and control subjects (4.06±0.35 µg/ml, P-value=0.18). Subcutaneous adipose tissue retinol was lower significantly in BCC patients (38.60±3.30 ng/mg) compared with control group (54.78±3.49 ng/mg, P-value=0.002). Furthermore, results revealed lower subcutaneous adipose tissue ɑ-tocopherol in BCC patients (4.41±0.33 µg/ml) in comparison with control group (4.06±0.35 µg/ml, P-value=0.18). Conclusion: Skin tissue concentration of retinol and ɑ-tocopherol and serum retinol level was lower in BCC patients in comparison with control group but serum ɑ-tocopherol was not different between groups. https://ijph.tums.ac.ir/index.php/ijph/article/view/18542 https://ijph.tums.ac.ir/index.php/ijph/article/download/18542/6536

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1940 1941 Nahid NAJAFI Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AND Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran AND Department of Toxicology and Pharmacology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Mahmoud MONTAZERI Department of Autopsy, Fars Province General Office, Legal Medicine Organization, Shiraz, Iran 2019 10 05 Abstract  Abstract  Abstract  Abstract   https://ijph.tums.ac.ir/index.php/ijph/article/view/18567 https://ijph.tums.ac.ir/index.php/ijph/article/download/18567/6556

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1847 1854 Nasim ZAMANI Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Department of Clinical Toxicology, Loghman-Hakim Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hossein HASSANIAN-MOGHADDAM Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Department of Clinical Toxicology, Loghman-Hakim Hospital, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Alireza NOROOZI School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran Mohammad Bagher SABERI ZAFARGHANDI Department of Addiction, School of Behavioral Sciences and Mental Health, Tehran Institute of Psychiatry, Iran University of Medical Sciences, Tehran, Iran Ali-Asghar KOLAHI Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2019 10 05 Background: Substance use is a growing problem in many countries especially among teenagers. We aimed to estimate the prevalence and complications of substance use in teenagers referring to a tertiary hospital following intoxication. Methods: In a cross-sectional study between 2012 and 2013 in Loghman Hakim Hospital, Tehran, Iran, sixteen substances were checked in teenagers referring due to poisoning. They divided into two groups of young (13 to <16 yr; group 1) and old-teenagers (16 to 19 yr; group 2). History of lifetime substance use and drug use within the week prior to admission were recorded. Results: Mean (range) age of young teenagers was 14.5±0.6 [13, 15] and 17.7±1.1 [16, 19] in old-teenagers with female predominance. Of 264 teenagers, four in group 1 and 27 in group 2 were admitted due to drug overdose. Six and 69 patients in groups 1 and 2 claimed that they had used some kind of substance in the week prior to admission. Twenty (37%) and 106 (50.5%) patients in the young and old-teenager groups were defined as drug users and rates of unreported substance use were 27.8% (15 cases) and 23.8% (50 cases) respectively. Ninety-six substance users (36.4%) had referred due to poisonings other than recreational intoxication (P<.001). Screening toxicological lab data showed significant opioid and sedative exposure in old-teenagers. Conclusion: It seems young adolescents hide their drug abuse more than old teenagers. Using illicit drugs screening tests may help us to provide hidden rate of abuse in teenagers. https://ijph.tums.ac.ir/index.php/ijph/article/view/18543 https://ijph.tums.ac.ir/index.php/ijph/article/download/18543/6537

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1942 1944 Kabir Ozigi ABDULLAHI Department of Medico-Surgical Nursing, School of Nursing & Midwifery, Tehran University of Medical Sciences, Tehran, Iran Fatima Mahmud MUHAMMAD Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Kourosh HOLAKOUIE-NAIENI Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ahmad KHOSRAVI Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Fahad Saqib LODHI Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran hang Abdullah KAREEM Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hasnain SABAHAT Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Sami Najmaddin SAEED Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Ali HOSSEINI Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2019 10 05 Absracts Absracts Absracts Absracts https://ijph.tums.ac.ir/index.php/ijph/article/view/18568 https://ijph.tums.ac.ir/index.php/ijph/article/download/18568/6557

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1855 1860 Zeinab TAVAKKOL AFSHARI Department of Immunogenetic and Cell Culture, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Zahra GHOLIZADEH Department of Immunogenetic and Cell Culture, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AND Department of Allergy and Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Amin Reza NIKPOOR Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Jalil TAVAKKOL AFSHARI Department of Immunogenetic and Cell Culture, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AND Department of Allergy and Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Rashin GANJALI Department of Immunogenetic and Cell Culture, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Fatemeh HOMAEI SHANDIZ Cancer Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Khadijeh JAMIALAHMADI Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran AND Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2019 10 05 Background: The tumor protein p73 (TP73) is a homolog of TP53 family. Ectopic p73 overexpression largely mimics p53 activities as a tumor suppressor and activates the transcription of p53-responsive genes and as a result induce apoptosis. This study aimed to investigate the association between p73 G4A polymorphism and the risk of breast cancer in a northeastern Iranian population. Methods: This case-control study was performed on 105 patients who admitted in educational hospitals of Mashhad University of Medical Sciences, Iran during 2013-2015, with breast cancer as case group and 120 healthy women as the control group. PCR-CTPP method was used to investigate the relationship between the p73 G4A polymorphism and the risk of breast cancer. Results: There was no significant association between the AA genotype of the p73 G4A polymorphism and breast cancer in case and control groups. Although G allele frequency was higher in the case group, the abundance of this allele between case and control groups was not statistically meaningful and, as a result, not associated with the risk of breast cancer in this study group. Conclusion: There was no association between G4A p73 polymorphism and the risk of breast cancer in a northeastern Iranian population. https://ijph.tums.ac.ir/index.php/ijph/article/view/18545 https://ijph.tums.ac.ir/index.php/ijph/article/download/18545/6538

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1861 1869 Morteza JOSHANI KHEIBARI Social Determinants of Health Research Center, Gonabad University of Medical Sciences, Gonabad, Iran Reza ESMAEILI Department of Public Health, School of Health, Social Development and Health Promotion Research Center, Gonabad University of Medical Sciences, Gonabad, Iran Mahmood KAZEMIAN Department of Health Economics, School of Medicine, Shahed University, Tehran, Iran 2019 10 05 Background: Health reform in Iran began in 2014, aimed at improving financing pattern of health services. We assessed the reform by changes in variables representing distribution of health payments and catastrophic expenditures. Methods: Using data from households’ income-expenditure survey, this study computed the financial variables, representing poverty line and households at poor state, household’s catastrophic health expenditure, fairness in financial contribution (FFC) index, and household’s impoverishment state, in the years 2010-2016, in urban and rural areas. The variables were computed by special software designed for this study, based on C-Sharp(C#) programming language, with yearly data on more than 38000 households, each with 1072 information sources. Results: The food share-based poverty line after sharp rise in 2010-2013, in 2014-2016 raised slowly, and the average percent of households facing catastrophic health expenditure, after sharp rise in 2011-2013, left at 3.25 in 2014-2015 and raised to 3.45 in 2016. The average FFC index remained at 0.839 to 0.837 in 2013-2016. However, interestingly, the average percent of households impoverished after out-of-pocket payments improved from 1.36 to 0.912 in 2013-2016. Conclusion: In three years of health reform, the major impact of reform was considerable improvements in the rate of the impoverished after out-of-pocket payments. The reform had limited impacts on the rates of households facing catastrophic health expenditure, and on FFC indexes, for the rural and urban residents. https://ijph.tums.ac.ir/index.php/ijph/article/view/18546 https://ijph.tums.ac.ir/index.php/ijph/article/download/18546/6539

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1870 1878 Fatemeh ESFARJANI Department of Food and Nutrition Policy and Planning Research, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Hedayat HOSSEINI Department of Food Science and Technology, National Nutrition & Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ramin KHAKSAR Department of Food Science and Technology, National Nutrition & Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Roshanak ROUSTAEE Department of Food and Nutrition Policy and Planning Research, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Haleh ALIKHANIAN Department of Food and Nutrition Policy and Planning Research, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Marjan KHALAFI Department of Food and Nutrition Policy and Planning Research, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran Amin MOUSAVI KHANEGHAH Department of Food Sciences, Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, Sao Paulo, Brazil Fatemeh MOHAMMADI-NASRABADI Department of Food and Nutrition Policy and Planning Research, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2019 10 05 Background: Food safety and food security are interrelated concepts with a profound impact on the quality of human life. The current study, for the first time, was set to identify associations between home food safety practice and household food insecurity a structural equation modeling approach. Methods: In this cross-sectional study, urban households were selected from among 10 health centers of five districts of Tehran, Iran (2015). The following questionnaires were completed: socioeconomic status (SES), food security and food safety. Structural Equation Modeling (SEM) was applied for predicting the relationships between SES, food insecurity, and food safety in households. Results: Food security was observed in 56% of households. Mild, moderate and severe food-insecure households were determined to be 29%, 12%, and 3%, respectively. In addition, the scores of home food safety practice in 37.5%, 33% and 29.5% of the households were classified as desirable, acceptable and weak, respectively. Low-educated mothers having husbands with low educational and occupational level had a weaker food safety practice compared to high-educated ones. Based on the SEM results, an inverse association between food safety practice and food insecurity score was observed (t= -2.89, ɣ= 0.16). Conclusion: Food insecurity and undesirable food safety practice were relatively prevalent among households. In addition, the economic and social factors could inversely affect both food insecurity and food safety practices. https://ijph.tums.ac.ir/index.php/ijph/article/view/18549 https://ijph.tums.ac.ir/index.php/ijph/article/download/18549/6540

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 10 2019 10 05 1879 1884 Reza RANJBAR Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Davoud AFSHAR Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 2019 10 05 Background: Klebsiella pneumonia is one of the common causes of pneumonia and bacteremia in intensive care patients. The present study was aimed to determine the capability of (GTG) 5-PCR assay for molecular typing of K. pneumonia strains isolated from patients with urinary tract infections. Methods: In this descriptive-sectional study, K. pneumoniae strains were collected from hospitalized patients with urinary tract infection in Baqiyatallah Hospital, Tehran, Iran during 2017-2018. Isolates were identified by conventional microbiological tests. Bacterial DNA was extracted using boiling method and (GTG) 5-PCR assay w