Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 1 6 M Moin N Rezaei M Yeganeh 2015 10 03 The UNESCO Chair in Health Education in TUMS (TUMS), Iran, was established in Immunology, Asthma and Allergy Research Institute in April 2004. The purpose of this chair is to promote an integrated system of research, training, information and documentation activities in the field of health education. The target group includes the public, health care workers, students, trainees and researchers. During its one year existing, the Chair has supported financially eleven admitted proposals and supported technically eight submitted proposals in divers fields regarding health issues with the cooperation of other research institutes in TUMS. It has made connections with most of the national research institutes and universities to establish multi-centric collaborations. The activities of the Chair are being directed towards the health priorities of the country and region. The Chair has contributed to an important international congress on immunodeficiency disorders as well as another meeting on Asthma in near future. The Chair is to publish several educational books, booklets and CDs related to health education; it is also going to prepare a thorough proposal on HIV/AIDS prevention with collaboration of other institutions, which could be applicable regionally, and in neighboring countries and states. https://ijph.tums.ac.ir/index.php/ijph/article/view/2171 https://ijph.tums.ac.ir/index.php/ijph/article/download/2171/2152

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 7 15 F Sahin Mutlu U Ayranci K Ozdamar 2015 10 03 Smoking has become more prevalent in Turkey than it has in those of western countries during the past decade. This study was conducted to make parameter estimations on gender related smoking habits with the minimum of variance. Of the ninety-two researches related to smoking habits conducted from 1981 to 2003 in Turkey, 60 were deemed appropriate for the application of Meta analysis and Meta regression analysis. The proportions of men and women smoking cigarettes were 0.51 and 0.35, respectively. The proportion of men smoking cigarette in 1996 and the years before it was 0.52, and for women as 0.35. However, the figures for the years following 1996 were 0.41 for men, and 0.32 for women. In the results of the Dersimonian and Laird random effect model, the Odds Ratio, which shows the tendency of men to smoke compared to women, was found 1.894 for the period of 1981-2003. A heterogeneous distribution between the researches was apparent (Q=1560.91, P https://ijph.tums.ac.ir/index.php/ijph/article/view/2172 https://ijph.tums.ac.ir/index.php/ijph/article/download/2172/2153

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 16 24 A Karami P Hindersson N Høiby S Morovvati 2015 10 03 Lyme borreliosis is a tick-born disease caused by the spirochete Borrelia burgdorferi. The aim of this research was molecular typing of different strains of B. burgdorferi and protective efficacy of outer surface protein in animal model. Seven strains of B. burgdorferi isolated from skin and CSF of Lyme disease patients were compared using PCR- RFLP as well as DNA and amino acid sequences. The gene coding for Outer Surface Protein A (OspA) was cloned and recombinant protein was expressed. Immunization experiment was conducted on gerbils with purified recombinant OspA protein. The OspA gene sequence of B. afzelii and B. garinii showed 83.76% similarity and calculated amino acid identity of 84 % with each other and 82.5% similarity to B31 strain. DK6 was 100% similar to Pbi, 99.6 % to Ptrob isolate and 88 % to B31 strain. Cloning and expression of OspA from DK6 strain in E. coli has shown lipoprotein with molecular weight of 32 kDa. Immuno blot of recombinant protein from DK6 with rabbit anti DK1 revealed strong reaction with OspA from DK6 strain. Immunization experiment in gerbils with three doses of rOspA protein revealed that all immunized animals either with OspA fusion protein alone or combined with adjuvant were protected against infection with DK1 or DK6 pathogenic strains. https://ijph.tums.ac.ir/index.php/ijph/article/view/2173 https://ijph.tums.ac.ir/index.php/ijph/article/download/2173/2154

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 25 28 R Yazdani M Oshaghi A Havayi E Pishva R Salehi M Sadeghizadeh H Foroohesh 2015 10 03 Wound infections are a common cause of staphylococcal infections. An ability of S.aureus is to adhere and form biofilm on host surfaces. Biofilm is an exopolysaccharide, a slime matrix around multiple layers of cells and is mediated by expression of the icaADBC operon. The present study evaluated the biofilm forming capacity and the presence of icaAD gene among S.aureus isolated from wound infections. Slime production assay was performed by cultivation on Congo Red Agar plate. In addition, Quantitative biofilm formation determined by microtiter plate assay PCR method used for detection of icaAD gene. Fifty strains were identified, 54% of the isolates produced black colonies on CRA plate, 52% were positive biofilm forming, and all strains carried the icaAD gene. Regarding the ability of S.aureus to form biofilms helps the bacterium to survive hostile environments within the host, suggests that biofilm production is a risk factor for infection. It is important in rapid diagnosis and treatment biofilm forming strains, because biofilm formation may lead to increased antimicrobial resistance and create a significant impediment to wound healing. https://ijph.tums.ac.ir/index.php/ijph/article/view/2174 https://ijph.tums.ac.ir/index.php/ijph/article/download/2174/2155

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 29 32 MB Rokni S Lesan Massoud J EB Kia Molawi Gh 2015 10 03 Fast enzyme linked immunosorbent assay (Fast-ELISA) was compared with the standard ELISA for the diagnosis of human hydatidosis. Seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), trichostrongylosis (n=5), and strongyloidosis (n=5) were analysed for anti-hydatid IgG antibodies using sheep hydatid cyst fluid antigen. The sensitivity, specificity, positive and negative predictive values, as well as validity of the test were found as 96.7%, 95.2%, 93.7%, 97.5% and 96% for conventional ELISA, while these paramters for fast-ELISA were respectively as follows: 100%, 97.5%, 96.7%, 100% and 98.8%. Regarding standard-ELISA 3µg/ml of antigen, serum dilution of 1:500, conjugate dilution of 1:3000 and 30 min incubation were found optimal, while for fast-ELISA 3µg/ml of antigen, serum dilution of 1:125, conjugate dilution of 1:1000 and 5 min incubation were utilized. The present study indicates that fast ELISA can easily be performed in place of the standard ELISA for the serodiagnosis of human hydatidosis with the advantage of minimising consumed time and manpower hours. Moreover, this test can be utilized in screening tests to diagnos human hydatidosis. https://ijph.tums.ac.ir/index.php/ijph/article/view/2175 https://ijph.tums.ac.ir/index.php/ijph/article/download/2175/2156

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 33 37 S Rezaie H Khodadadi F Noorbakhsh MR Safari 2015 10 03 Trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. Several properties of this fungus have been investigated so far. However, a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the subunit G of its vacuolar ATPase (V-ATPase). Pairs of 21 nt primers were designed from highly conserved regions of the V-ATPase subunit G genes in other fungi. Mentioned primers were utilized in PCR using isolated genomic DNA template as well as cytoplasmic RNA of T.rubrum and the PCR and RT-PCR fragments were then sequenced. About 469 nucleotides were sequenced which encoded a polypeptide with 119 amino acids. Nucleotide sequence comparison in gene data banks (NCBI, NIH) for both the DNA and its deduced amino acid sequence revealed significant homology with V-ATPase subunit G genes and proteins of other eukaryotic cells. The amino acid sequence of the encoded protein was about 84% identical to the sequence of V-ATPase subunit G from other fungi. In summary, we have cloned the first V-ATPase subunit G of dermatophytes and characterized it as a member of this gene family in other eukaryotic cells. https://ijph.tums.ac.ir/index.php/ijph/article/view/2176 https://ijph.tums.ac.ir/index.php/ijph/article/download/2176/2157

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 38 41 F Agha-Hosseini F Tirgari S Shaigan 2015 10 03 Some lesions in the oral cavity and mostly on gingiva have predominant predilection towards females, and mostly occur in the first four decades of life when changes in sex hormone levels in blood are obvious. The present study aimed to investigate the presence and distribution of estrogen and progesterone receptors in peripheral giant cell granuloma (PGCG), pyogenic granuloma (PG) and peripheral ossifying fibroma (POF) on gingiva as an organ target. In a descriptive case series study from March 2002 to April 2003, paraffin blocks from patients with exophitic lesion on gingiva, diagnosed by histopathology as PGCG, PG or POF at Dentistry Faculty of Tehran University of Medical Sciences (TUMS), Iran, were analyzed with Immunohistochemical (IHC) technique. The data analysis was performed by frequency and descriptive statistics. Of 35 patients, 12 estrogen receptors (ERS) and progesterone receptors (PRS) were detected. Nine of them were PRS and three were ERS. Two third of ERS/ PRS were seen in females and one third in males, respectively. In order of decreasing frequency the ERS and PRS were found in PG (n=6), POF (n=4) and PGCG (n=2). In this study, ER/ PR were revealed in three lesions. PR was detected in all of three lesions but we could not see ER in PGCG. Thus, gingiva may be considered as a target organ for sex hormones. https://ijph.tums.ac.ir/index.php/ijph/article/view/2177 https://ijph.tums.ac.ir/index.php/ijph/article/download/2177/2158

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 42 48 AM Alavi Naini M Amini M Karajibani AL Khalilian S Nourisaeedloo M Salimi KH Shafaghi J Yhoung-aree 2015 10 03 In order to assess the relationship between obesity with food habit and body image, this case control study was conducted among 85 urban primary school children male and female aged 10-12 years old, attending Anuban School in Nakhon Pathom Province, in Thailand. Two different questionnaires were used for data collection for caregivers and students. The standard used for definition of overweight and obesity was body mass index [BMI]. Obesity was defined as percentile ≥ 95th of the sex-specific BMI-for-age growth chart, CDC. Obese children commonly (> 4 times per week) ate blanched /steamed foods as well as spicy foods. While sugar consumption was the same between the two groups, oil consumption was higher among the obese group. Compared with control children, obese children had more snacks before breakfast as well as during lunch and dinner on weekdays and weekends. Normal students had more foods- except for leafy vegetable and fried BBQ snacks. Two-thirds of the children knew about the food groups, but only one-third of them could classify foods into the food groups. Obese children knew about the food groups and could classify foods better than the way normal children could. Children gained information about foods from their parents, teachers, sisters, books, and television. Most of obese children knew they were obese, and they were not happy with their status. In conclusion, Programs must be designed to improve nutrition and in this way, parents and /or caregivers must be involved. https://ijph.tums.ac.ir/index.php/ijph/article/view/2178 https://ijph.tums.ac.ir/index.php/ijph/article/download/2178/2159

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 2 2006 06 15 49 56 Health Management and Economics Research Center, Iran University of Medical Sciences, Tehran, Iran Majeed NAKHAEE Dept. of Health Services Management, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran Farideh MORADI Life Style Modification Research Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran 2018 03 04 2018 03 04 No Abstract### https://ijph.tums.ac.ir/index.php/ijph/article/view/12698 https://ijph.tums.ac.ir/index.php/ijph/article/download/12698/5935

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 47 3 2018 03 04 350 356 Phuong Kim TRUONG Dept. of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Vietnam AND National University, Ho Chi Minh, VietnamDept. of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh, Vietnam Thuan Duc LAO Dept. of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh, Vietnam Thuy Ai Huyen LE Dept. of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh, Vietnam 2018 03 04 2018 03 04  Background: The infection of human papillomavirus (HPV) has been considered as the common cause of cervical cancer, which is the leading cause of cancer death in women, in Vietnam. Recently, hypermethylation at tumor suppressor genes (TSGs) has been also demonstrated to be an early epigenetic event and cofactor in human cancer, including cancer of cervix. This study evaluated the frequency of DcR1 gene promoter hypermethylation status as well as whether did or not an association between patterns of DNA hypermethylation and high-risk HPV infection, led to risk of cervical cancer. Methods: Methylation-Specific-PCR (MSP) was performed to analyze hypermethylation status from 109 liquid-based Papanicolaou test samples, archived and admitted from the Medic Medical Center and Au Lac Clinic Laboratory, Vietnam, from 2011–2014, a kind of non-invasive samples identified whether HPV/or non-HPV, high-risk/low-risk HPV infection. Results: DcR1 promoter was differentially methylated in 50% cases of high-risk HPV genotype 16 and 18 infected samples. In contrast, a low frequency of hypermethylated DcR1 promoter was found in low risk HPV genotype infected sample (16.0%), and non-HPV infected sample (14.6%). A trend toward positive association was found between hypermethylation of DcR1 gene and HPV exposure was observed (P=0.0005). Moreover, the odds ratio (OR) and relative risk (RR) were found in statistical significant value (OR=5.63 (95%CI = 2.25 - 14.07, P<0.01), RR=3.31 (95%CI = 1.75 - 6.26, P<0.01)). Conclusion: The hypermethylation of DcR1 gene promoter is a significant characteristic of high-risk HPV infected samples in Vietnamese cervical patients. The OR and RR values showed that the strong correlation between DcR1 hypermethylation and high-risk HPV infection, in which increased the risk of cervical cancer. The combination of DcR1 hypermethylation and HPV detection based biomarker could be used in non-invasive samples obtained from high-risk cancer patients, offer significant practical advantages.     https://ijph.tums.ac.ir/index.php/ijph/article/view/12681 https://ijph.tums.ac.ir/index.php/ijph/article/download/12681/5920

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 47 3 2018 03 04 455 457 Raziyeh KARAMI ESHKAFTAKI Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Effat FARROKHI Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Fatemeh HEYBATI GOJANI Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Najmeh SALEHI VANANI Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Maryam KARAMI ESHKAFTAKI Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Ezzatollah MEMARZADEH Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Morteza HASHEMZADEH CHALESHTORI Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran 2018 03 04 2018 03 04 No Abstract### https://ijph.tums.ac.ir/index.php/ijph/article/view/12699 https://ijph.tums.ac.ir/index.php/ijph/article/download/12699/5936

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 47 3 2018 03 04 357 366 Yat YEN School of Public Affairs, University of Science and Technology of China, Hefei, China AND Dept. of Social Work, National Institute of Social Affairs, Phnom Penh, Cambodia Yumin SHI School of Public Affairs, University of Science and Technology of China, Hefei, China Bunly SOEUNG Faculty of Social Sciences, Svay Rieng University, Svay Rieng, Cambodia Rathny SUY School of Public Affairs, University of Science and Technology of China, Hefei, China Muhammad Tayyab SOHAIL School of Public Administration, China University of Geosciences (Wuhan), Wuhan, Hubei, China 2018 03 04 2018 03 04 Background: We examined the current prevalence of substance abuse and sexual behaviors among junior high school students and the relationships between substance abuse, sexual behaviors, and victimization using multiple mediations. Methods: Overall, 1703 junior high school students from the 2013 Cambodia Global School-based Health Survey were selected for the study. The descriptive statistics were performed in IBM SPSS to determine the prevalence of substance abuse, sexual behaviors, and victimization. The Process Macro was installed in Regression of the SPSS to test the hypotheses and mediations. Results: The majority of students who used alcohol (15.4%), drugs (3.05%), and had sexual intercourse (12.45%), were male aged 14-15, and in grade 7. These students were very vulnerable to many risky behaviors, including bullying (22.20%), physical attacks (20.96%) and fights (14.50%), unintentional accidents (21.32%), and suicidal attempts (5.05%). All three hypotheses were significantly supported. Of the potential mediators examined, drug use is the most important mediator. Conclusion: The substance abuse and reproductive health are national problems, but abusive behaviors among students are of particular concern. Explicit policies and awareness programs of such problems at the high school level need to be made and called for public participation, particularly the school authorities and parents.   https://ijph.tums.ac.ir/index.php/ijph/article/view/12682 https://ijph.tums.ac.ir/index.php/ijph/article/download/12682/5921

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 47 3 2018 03 04 458 459 Mahtab KARAMI Health Information Management Research Center, Dept. of Health Information Technology and Management, School of Allied-Medical Sciences, Kashan University of Medical Sciences, Kashan, Iran Ali HOSSEINI SHAHMIRZADI Primary Healthcare System, SART Distract PHC Center, Mazandaran University of Medical Sciences, Sari, ‎Iran 2018 03 04 2018 03 04 No Abstract### https://ijph.tums.ac.ir/index.php/ijph/article/view/12700 https://ijph.tums.ac.ir/index.php/ijph/article/download/12700/5942

Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 47 3 2018 03 04 367 372 Jian ZHANG Dept. of Obstetrical, Binzhou Central Hospital, Binzhou, Shandong, Chi