Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 46 1 2017 01 25 23 34 Zhen DING Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China Jintao LI Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China AND State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China Fan LI Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China AND State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China Mohammadreza Mohammadzad MEPHRYAR Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China AND State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China Shuicai WU Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China Chen ZHANG China Academy of Telecommunication Research of Ministry of Industry and Information Technology, Beijing, 100191, China Yi ZENG Beijing Key Laboratory of Environmental and Viral Oncology, School of Life Science and Bio-Engineering, Beijing University of Technology, Beijing, 100124, China AND State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China 2017 01 25 2017 01 25 Background: The extremely low-frequency electromagnetic field (ELF-EMF), mainly emitted by electric transmission lines and household electronic appliances, is becoming a worldwide health risk. It is imperative to investigate the biological impacts of ELF-EMF and to identify products that are resistant to the radiation from 50 Hz ELF-EMF. In this study, we investigated the biological impacts of apoptosis caused by 50 Hz Power line ELF-EMF and the protective effects of Vit C and Vit E. Methods: We conducted this study in Beijing, China in 2013. B95-8  and Balb/c-3T3 cells were divided into a sham group, an expo group and 3 expo groups in which the cells were preincubated with various concentrations of Vit C and Vit E. Then, all of the cells were exposed to 50 Hz Power line ELF-EMF and examined for apoptosis. The cells were collected for apoptosis detection after exposure. Results: The percent of cells that undergoing apoptosis and preincubated with various concentrations of Vit C and Vit E were significantly lower than in the Expo group. Conclusion: Vit C and Vit E exert significant protective effects from 50 Hz ELF-EMF radiation. The optimal protective concentrations of Vit C and Vit E are 10 μmol/L and 25 μmol/L, respectively. The protective effect of vitamins was more apparent for Balb/c-3T3 cells than B95-8 cells.   https://ijph.tums.ac.ir/index.php/ijph/article/view/8914 https://ijph.tums.ac.ir/index.php/ijph/article/download/8914/5507