Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 54 2 2025 02 08 297 308 Lingfei Ma Department of Nursing, Jiaozhou Central Hospital of Qingdao, Qingdao, 266300, China Fuping Ye Department of Gastrointestinal Surgery, Jiaozhou Central Hospital of Qingdao, Qingdao, China 2024 07 27 2024 10 21 Background: Nosocomial infections represent a critical challenge in nursing practice, particularly during the COVID-19 pandemic. Isolation measures have been implemented widely to curb the spread of infections within healthcare settings. We aimed to evaluate the effectiveness of isolation measures in reducing nosocomial infection rates in nursing practice during the COVID-19 pandemic. Methods: A systematic search was conducted using keywords such as "isolation measures," "nosocomial infection," and "COVID-19" and their combinations in international databases, focusing on articles published between 2020 and 2024. Data were analyzed using meta-analysis and a random effects model. Heterogeneity between studies was assessed with the I² test, and analyses were performed using STATA software. Results: The analysis of 8 selected articles with a total sample size of 10,532 individuals showed that the implementation of isolation measures significantly reduced nosocomial infection rates, with an average effect size of 0.58 (95% CI: 42.1-74.5). Conclusion: Isolation measures are effective in reducing nosocomial infection rates in nursing practice during the COVID-19 pandemic. The findings support the continued use of these measures to enhance patient safety and control infection spread within healthcare facilities. https://ijph.tums.ac.ir/index.php/ijph/article/view/36188 https://ijph.tums.ac.ir/index.php/ijph/article/download/36188/8465