Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 44 5 2015 05 15 654 8 Alireza Mohammadi Dept. of Medical Parasitology and Mycology, School of Public Heath, Tehran University of Medical Science, Tehran, Iran. Saeedeh Shojaee Dept. of Medical Parasitology and Mycology, School of Public Heath, Tehran University of Medical Science, Tehran, Iran. Mahboobeh Salimi Dept. of Medical Parasitology and Mycology, School of Public Heath, Tehran University of Medical Science, Tehran, Iran. Mehdi Zareei Dept. of Medical Parasitology and Mycology, School of Public Heath, Tehran University of Medical Science, Tehran, Iran. Mehdi Mohebali Dept. of Medical Parasitology and Mycology, School of Public Heath, Tehran University of Medical Science, Tehran, Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran. Hossein Keshavarz Dept. of Medical Parasitology and Mycology, School of Public Heath, Tehran University of Medical Science, Tehran, Iran. 2015 10 14 Background: Toxoplasmosis is a zoonotic and usually asymptomatic infection. This study was carried out to investigate the seroepidemiology of Toxoplasma infection in women referred to Arak Marriage Consulting Center during 2012-2013. Methods: In this cross-sectional study, serum samples were collected from 400 women referred to Marriage Consulting Center in Arak City, Markazi Province, central Iran during 2012-2013. Anti-Toxoplasma IgG and IgM antibodies were measured by ELISA using homemade antigen. Results were analyzed by SPSS 18 and the correlation between toxoplasmosis and some affecting factors were estimated. Results: Overall, 97 cases (24.3%) had IgG antibodies against T. gondii and 19 cases (4.8%) were positive for IgM antibodies. A significant correlation was seen between T. gondii infection with clinical symptoms, keeping cat as pet animal, education and handling or eating raw or undercooked meat (P < 0.05). Conclusion: The prevalence of (24.3%) for Toxoplasma infection was seen in Arak City. It seems that keeping cat as pet and consumption of undercooked liver and uncooked hamburger are the most important transmission routes for the infection in this city. Since the majority of women are Toxoplasma sero-negative (75.7%) in Arak City, using serological tests and health education prior to marriage or during pregnancy is recommended. https://ijph.tums.ac.ir/index.php/ijph/article/view/3552 https://ijph.tums.ac.ir/index.php/ijph/article/download/3552/3227